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Microbiology Lecture Exam #1
Presentations 3, 4, & 5
122
Microbiology
Not Applicable
02/02/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Type of bacteria that lives in harsh conditions.
Definition
Archaea
Term
Cells of animals, plants, fungi, and protist
Definition
Eukaryotic Cells
Term
Contain membrane-bound organelles that compartmentalize the cytoplasm and perform specific functions.
Definition
Eukaryotic cells
Term
No nucleus or other membrane-bound organelles.
Definition
Prokaryotic Cells
Term
Contain double-membrane bound nucleus with DNA chromosomes.
Definition
Eukaryotic cells
Term
Basic shape of cells
Definition
spherical, cubical, and cylindrical
Term
Internal content
Definition
cytoplasm, surrounded by a membrane...DNA chromosome(s), ribosomes(synthesized protiens), metaboic capabilities.
Term
1st Characteristic of Life
Definition
Reproduction and heredity-genome composed of DNA packed in chromosomes; produce offspring sexually or asexually.
Term
2nd characteristic of life
Definition
Growth and development
Term
3rd characteristic of life
Definition
Metabolism-chemical and physical life processes
Term
4th chacteristic of life
Definition
Movement and/or irritability-respond to internal/external stimuli; self-propulsion of many organisms.
Term
5th characteristic of life
Definition
cell support, protection, and storage mechanisms - cell walls, vacuoles, granules and inclusions
Term
6th characteristic of life
Definition
Transport of nutrients and waste
Term
Cellular structure and function is amazingly versatile and adaptable
Definition
Prokaryotic cell
Term
Appendages, flagella, Pili, Fimbriae, Glycocalyx-capsule, slime layer
Definition
Prokaryotic cell (external)
Term
Cell wall, cell membrane
Definition
Prokaryotic cell (cell envelope)
Term
cytoplasmic matrix, ribosomes, inclusions(stored material), nucleoid/chromosome(DNA), actin cytoskeleton, endospore (has crust coating and is entirely different metabolic state)
Definition
Prokaryotic cell (Internal)
Term
Because of their small size prokaryotic cells...
Definition
appear featureless and two dimensional when viewed with an ordinary microscope.
Term
category of prokaryotes with pepidoglycan in their cell walls
Definition
Bacteria
Term
organizing, classifing, and naming living things is called
Definition
Taxonomy
Term
orderly arrangement of organisms into groups is called
Definition
classification
Term
assigning names is called
Definition
nomenclature
Term
determining and recording traits of organisms for placement into taxonomic schemes...
Definition
identification
Term
Levels of Classification...1,2,3
Definition

1. Domain (Archaea, Bacteria, & Eukarya)

2. Kingdom

3. Phylum or Division

Term
Levels of Classification 4,5,6
Definition

4. class

5. order

6. family

Term
Levels of classification 7,8
Definition

7. Genus

8. Species

Term
Plants, Animals, Fungi, Protists
Definition
Domian Eukarya
Term
Cyanobactera, Chlamydias Spirochetes, Gram-positive bacteria, Endospore producers, Gram-negative bacteria
Definition
Domain Bacteria
Term
Binomial (scientific) nomenclature
Definition

Geives each microbe 2 names:

Genus - which is captialized

species - which is lower case

Both of which are italicized or underlined

Term
natural relatedness between groups of organisms is called
Definition
Phylogeny
Term

-All new species originate from preexisting species.

-Closely related organism have similar features because they evolved from common ancestral forms.

Definition
Evolution
Term
Methane producers, Prokaryotes that live in extreme salt, Prokaryotes that live in extreme heat
Definition
Domain Archaea
Term
true bacteria
Definition
Bacteria
Term
odd baceria that live extreme environments, high salt, heat, etc.
Definition
Archaea
Term
Have a nucleus and organelles.
Definition
Eukarya
Term
Process by which chemical substances (nutrients) are acquired from the environment and used in cellular activites.
Definition
Nutrition
Term
Must be provided to an organism
Definition
Essential Nurtients
Term

These nutrients are required in large quantities; play principal roles in cell structure and metabolism.

* Proteins, carbbohydrates

Definition
Macronutrients
Term

The nutrients are required in small amounts; involved in enzyme function and maintenance of pretein structure.

*Manganese, zinc, nickel

Definition
Micronutrients or trace elements
Term

Contain carbon and hydrogen atoms and are usually the products of living things.

 *Methane (CH4), carbohydrates, lipid, proteins, and nucleic acids

Definition
Organic nutrients
Term

Atom or molecule that contains a combination of atoms other than cargon and hydrogen (no carbon)

*Metals and their salts, gases, and water

Definition
Inorganic nutrients
Term

70% water

Proteins

96% of cell is composed of these 6 elements:

 

 

Definition

Carbon

Hydrogen

Oxygen

Phosphorous

Sulfur

Nitrogen

 

 

Term
Must obtain carbon in an organic form made by other living organisms such as proteins, carbohydrates, dipids, and nucleic acids.
Definition
Heterotroph
Term
Producers of an Eco-system
Definition
Autotroph
Term

an organism that uses CO2 an inorganic gas as its carbon source.

*Not nutritionally dependent on other living things.

Definition
Autotroph
Term
Organic compounds that cannot be synthesized by an organism because they lack the genetic and metabolic mechanisms to synthesize them
Definition
Essential Organic Nutrients
Term
Growth factors must be provided as a nutrient such as...
Definition
Essential amino acids and vitamins
Term
Sources of Essential Nutrients
Definition

Carbon sources

(Heterotroph and Autotroph)

Term
Main determinants of nutritional type are:
Definition

Carbon source-heteroph and autotroph

Energy source-chemotroph and phototrophs

Term
gain energy from chemical compounds
Definition
Chemotroph
Term
gain energy through photosynthesis
Definition
Phototrophs
Term
Photoautotroph is an autotroph that gets its CO2 from what energy source
Definition

Sunlight

ex: organisms, such as algae, plants, cyanobacteria

Term
Chemoautotroph is an autotroph that gets it CO2 from what energy source
Definition

Simple inorganic chemicals

ex: Only certain bacteria, such as methanogens, deep-sea vent bacteria

Term
Chemoheterotroph is an Hetetotroph/Organic that get its energy source from
Definition

Metabolic conversion of the nutrients from other organisms

ex: Protozoa, fungi, many bacteria, animals

Term
What is a Saprobe (Chemoheterotroph) energy source
Definition

Metabolizing the organic matter of dead organisms

ex: Fungi, bacteria (decomposers)

Term
What is a Parasite (Chemoheterotroph) energy source
Definition

Utilizing he tissues, uids of a live host

ex: Various parasites and pathogens; can be bacteria, fungi, protozoa, animal.

Term
A Photoheterotroph (Hetertroph/organic) that gets its energy from
Definition

Sunlight or organic matter

ex:  Purple and green photosynthetic bacteria

Term
Photoautotrophs survive by
Definition

Oxygenic photosynthesis and

Anoxygenic photosynthesis

Term
Chemoautotrophs (lithoautotrophs) survive
Definition
totally on inorganic substances
Term
Methanogen is a kind of chemoautotroph that...
Definition
produce methane gas under anaerobic conditions
Term
Majority of Heterotrophs are chemohetertrophs which survive on...
Definition
Aerobic respiration
Term

Free-living microoorganisms that feed on organic detritus from dead organisms

*Opportunistic pathogen

*Facultative parasite

are called:

Definition
Saprobes
Term

They derive nutrients from host

*Pathogens

*Some are obligate parasites

Definition
Parasites
Term
Type of transport that does not require energy; substances exist in a gradient and move from areas of higher concentration toward areas of lower concentration:
Definition
Passive transport
Term
List types of passive transports:
Definition
Diffusion, Osmosis, and Facilitated diffusion
Term
moves down concentration gradient
Definition
Diffusion
Term
diffusion of water
Definition
Osmosis
Term
requires a carrier
Definition
Facilitated diffusion
Term
Type of transport that requires energy and carrier proteins: gradient independent:
Definition
Active transport
Term
transported molecule chemically altered
Definition
Group translocation
Term
endocytosis (moves into the cell), exocyosis (goes outside fo the cell), pinocytosis
Definition
Bulk transport
Term
6 I's  of culturing microbes
Definition
Inoculation, Isolation, Incubation, Inspection, Information gathering, and Identification
Term
Introduction of a sample into a containter of media to produce a culture of observable growth is called
Definition
Inoculation
Term
Seperating one species from another is called
Definition
Isolation
Term
Under conditions that allow growth...
Definition
Incubation
Term
Isolation techniques include:
Definition

Streak plate technique

Pour plate technique

Spread plate technique

Term
Media can be classified by what three state:
Definition

Physical state

Chemical composition

Functional type

Term
liquid (TSB), simisolid, and solid
Definition
Physical state
Term
synthetic (chemically defined) and nonsynthetic (complex)
Definition
Chemical composition
Term
general purpose, enriched, selective, differential, anaerobic growth, specimen transport, assay, enumeration
Definition

Functional type

(Purpose of Medium)

Term
Type of media that can be chemically defined is called
Definition
Synthetic
Term
Type of media that is complex and is not chemically defined
Definition
Nonsynthetic
Term
liquid medium containing beef extract and peptone
Definition
Nutrient broth
Term
solid media containing beef extract, peptone (which is a partial digested protien), and agar
Definition
Nutrient agar
Term
The most commonly used solidifying agent is called
Definition
Agar
Term
A complex polysaccharide (sugars) isolated from red algae
Definition
Agar
Term
Agar is solid at room temperature, liquiefies at boiling (100 C), and does not re-solidify until it cools at____
Definition
42 C
Term
What does Agar do?
Definition
Provides framework to hold moisture and nutrients and is not digestivle for most microbes.
Term
Media that contains pure organic and inorganic compounds in an exact chemical formula
Definition
Synthetic
Term
Media that contains at least on ingredient that is not chemically definable.
Definition
Complex or nonsynthectic
Term
Media that grows a broad range of microbes, usually nonsynthetic.
Definition
General purpose media
Term
Media that contains complex organic substances such as blood, serum, hemoglobin, or special growht factors required by fastidious (hard to grow) microbes.
Definition
Enriched Media
Term
Contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes.
Definition
Selective Media
Term
Allows growth of several types of microbes and displays visible differences amound thos microbes.
Definition
Differential Media
Term
Contains a substance that absorbs oxygen or slows penetration of osygen into mediam; used for growing anaerobic bacteria.
Definition
Reducing medium
Term
Contains sugars that can be fermented converted to acids, and a pH indicator to show this reaction.
Definition
Carbohydrate fermentation medium
Term
Temperature-controlled chamber
Definition
Incubation
Term
observation; macroscopic and microscopic
Definition
Inspection
Term
grows only single known species of microorganisms
Definition
Pure culture
Term
hold two or more identified species or microoorganisms
Definition
Mixed cultures
Term
once pure or mixed uclture that has unwanted microbes growing
Definition
Contaminated culture
Term
macroscopic and microscopic appearance, biochemical test, genetic characteristics, immunological testing
Definition
Identification
Term
What two way are potentially hazardous cultures and specimens disposed?
Definition

-Steam Sterilization (Autoclave)

-Inceneration (Burning)

Term
Division of bacterial cells occurs mainly through
Definition
Binary fission
Term
Parent cell enlarges, duplicates its chromosome, and forms a central transverse septum dividing the cell into two daugher cells.  This is called
Definition
Binary Fission
Term
Time requied for a complete fission cycle is called the
Definition
generation or doubling time
Term
Each new fission cycle increases the population by a factor of
Definition
2-exponential growth
Term
Formula for calculating population size over time:
Definition

Nf = (N1)2n

 

Nf total # of cells in the population

N1 is starting number of cells

Exponentn denotes generation time

2n number of cells in that generation

Term
In laboratory studies, population typically display a predictable patter over time called
Definition
growth curve
Term
Stages in the normal growth curve include:
Definition
Lag Phase, Exponential growth phase, Stationary phase, and Death phase
Term
"flat" period of adjustment, enlargement; little growth is called
Definition
Lag Phase
Term
A period of maximum growth withll continue as long as cells have adequate nutrients and favorable environment.
Definition
Exponential growth phase
Term
Rate of cell growth equals rate of cell death caused by depleted nutrients and O2, excretion of organic acids and pollutants.
Definition
Stationary phase
Term
As limiting factor intensify, cells die exponentially.
Definition
Death phase
Term
Degree of cloudiness, turbidity, reflects the relative population size.  This is called
Definition
Turbidometry
Term
Getting a viable colony count or direct cell count of all cells present; automated or manual is called
Definition
Enumeration of bacteria
Term
What can pick up fine degrees of difference in turbidity?
Definition
Spectrophotometer
Term
Evenly cloudy thoughout
Definition
uniform fine turbidity
Term
produces a type of surface membrane
Definition
pellicle
Term
appears to clump (suspended chunks or pieces)
Definition
Flocculent
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