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Exam 1
MMBB 154
68
Microbiology
Undergraduate 4
02/09/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Who is the father of Microbiology, who first viewed "animalcules" (protozoa)?
Definition
Anton van Leeuwenhoek (1632 - 1723)
Term
1 Micron =
Definition
1/1000th of a mm
Term
The cell diameter of a Protozoa is?
Definition
100 microns
Term
The Cell diameter for Algae is?
Definition
10 - 100 microns
Term
The Cell diameter for Fungi is?
Definition
10 microns
Term
The Cell diameter for Bacteria is?
Definition
1 micron
Term
The Scientific Method
Definition

Observation

Hypothesis

Experiment (test hypothesis)

Intepretation 

Conclusion (approve/disprove)

Term
What is the doctrine that "lifeless substances give rise to living organisms"?
Definition
Spontaneous Generation
Term
What experiment was Francesco Redi famous for, and what period?
Definition

1670

Fransesco Redi demonstrated that maggots appearing "spontaneously" on old meat required the presence of flies

Term
What Experiment was Needham famous for and what period?
Definition

1748

Mutton Gravy in two flasks (Animalcules arise from spontaneous generation)

Term
What was Needham's experiment design mistake?
Definition

1) Air is full of Microorganisms

2) Air is needed for life

3) Heating the air to kill the microorganisms may be removing some vital "force"

Term
Who criticized Needham's work by using boiled infusions and heat-sealed glass vials using 4 Flasks with Mutton gravy?
Definition
Lazarro Spallanzani (1767)
Term
What did Needham counter to Spallanzani's criticism?
Definition
Spallanzani destroyed the vital force of life by excessive heating
Term
What is the vital force of life?
Definition
Oxygen
Term
Who invented a cure for Puerperal fever (childbed fever)?
Definition
Ignaz Semmelweis (1847)
Term
Who used variolation to find a vaccine for cowpox?
Definition
Edward Jenner (1796)
Term
John Snow was famous for what?
Definition

1854

Removed the street pump handle to start personal hygeine

(Epidemiology)

Term
Who disproved Spontaneous generation?
Definition

Louis Pasteur (1870)

microorganisms DO NOT arise from lifeless objects

Term
Who thought of the GERM THEORY OF DISEASE?
Definition
Louis Pasteur (1870)
Term
Who proved that yeast makes wine from grapes?
Definition
Louis Pasteur (1870)
Term
Who developed vaccine for rabies?
Definition
Louis Pasteur (1870)
Term
Who developed immunization techniques for chicken cholera and cholera?
Definition
Louis Pasteur (1870)
Term
Who PROVED  the Germ theory of disease?
Definition
Robert Koch (1875)
Term
What are the four Koch's postulates?
Definition

-1. Always observe organisms in diseased animals

-2. Isolate organism in pure culture

-3. Inoculate healthy animal and recreate the disea

-4. ReIsolate the organism 

Term
What is the purpose of Koch's postulates?
Definition
A sequence of experimental steps to relate a specific microbe to a specific disease
Term
Who developed "pure culture technique"?
Definition
Robert Koch (1875)
Term
What are some ways to reduce transmission of infectious diseases?
Definition
  • sterile practices in hospitals
  • pasteurization of dairy products
  • insect control
  • care in preparation of food
  • sanitation improvements
  • personal hygeine
Term
When is the Classical Golden Age of Microbiology?
Definition
1854 - 1914
Term

When is the Second Golden Age of Microbiology?

Definition
1943 - 1970
Term

When is the Third Golden Age of Microbiology?

Definition
NOW
Term
What is the most successful organism on the planet?
Definition
Pelagibacter ubique
Term
Microbes produce what amount of the oxygen we breathe?
Definition
50%
Term
What is the average human cell diameter?
Definition
10 microns
Term
What Cyanobacterium is responsible for 50% for the photosynthesis in open oceans?
Definition
Procholorococcus
Term
How many bacteria are ON and "IN" your body?
Definition
1014
Term
Who developed the five-kingdom system, giving bacteria their own kingdom?
Definition
Robert H. Whittaker and Lynn Margulis
Term
The Three-Domain system includes...
Definition
Bacteria, Eukarya, and Archaea
Term
Fungi Eukaryotes are composed of two groups...
Definition

Yeast (single cells)

Molds (multicellular)

Term
Most bacterial and archaeal cells are how long?
Definition
1 - 5 micrometers in length
Term
What domain contains some of the best studied prokaryotes?
Definition
Bacteria
Term
What tend to grow in hot, acidic, environments such as hot springs and volcanic vents?
Definition
Crenarchaeota
Term
How do Eukaryotes chemotax?
Definition
Spatial Sensing (take measurements at different ends of the cell simutaneously and move towards)
Term
How do Prokaryotes chemotax?
Definition
Temperal Sensing (take measurements of the concentration of a substance at two different time points)
Term
What is the purpose of the protective coat around cells in the Glycocalix?
Definition

A .Prevents dehydration

B .Prevents nutrients from flowing away

C .Prevents phagocytosis

Term
What is phagocytosis?
Definition
the cellular process of engulfing solid particles by the cell membrane to form an internal phagosome by phagocytes andprotists.
Term
Who determined the structure of the DNA molecule?
Definition
Watson and Crick (1953)
Term
What are plasmids?
Definition
not essential for replication or normal survival (extra chromosomal bacteria DNA)
Term
What is the process of polypeptide chain (protein)
Definition
Translation
Term
What copies genetic information into RNA?
Definition
transcription
Term
What enzyme sythesizes RNA from DNA?
Definition
RNA polymerase
Term
In 1976 the penecillin resistant strain of Neisseria gonorrhoeae is an example of what type of mutation?
Definition
Spontaneous Generation
Term
What is the inward pinching of hte cell membrane and cell wall that seperates the cell into two genetically identical cells?
Definition
cytokinesis
Term
Most bacteria grow at what pH?
Definition
Normal/pH 6.5 to 7.5
Term
What temperature does mesophiles grow best at?
Definition
20 - 45 degrees C
Term
What prokaryotic cell grows best at 40 - 70 degrees  C?
Definition
Thermophiles
Term
What is the optimal growth temperature for hyper - thermophiles?
Definition
80 degrees C
Term
The loss of fitness in Prokaryotes is defined as what?
Definition
senescence
Term
What are the five Catabolic Processes?
Definition

Glycolysis

Kreb's Cycle

Oxidative Phosphorylation

Anaerobic respiration

Fermentation

Term
When is the ATP bond holding the last phosphate group on the molecule broken, producing: adenosine diphosphate (ADP) + free phosphate group
Definition
When energy is released
Term
What is the first stage of energy extraction?
Definition
Glycolysis
Term
Glucose ---- Pyruvic Acid?
Definition
Glycolysis
Term
Pyruvic Acid ------- CO2?
Definition
Kreb's Cycle
Term
NADH/O2 --------- ATP/NAD/H20?
Definition
Oxidative Phosphorylation
Term
What is terminal Electron Acceptor?
Definition
When oxygen is the last molecule that accepts electrons
Term
What is the purpose of ATP synthase?
Definition
harnesses the energy as protons are passed by phosphorylating ADP into ATP
Term
What is it called when you have a set of Glycolysis, kreb's cycle, and oxidative phosphorylation?
Definition
Oxidative Respiration
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