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Exam 1 Anatomy and Physiology of Animals
Exam 1 Dr. Jones anat and phys
216
Biology
Undergraduate 3
09/12/2011

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Term
Dorsal Plane
Definition
Divides body on long access to front and back halves. Can also have cross section of a limb.
Term
Transverse plane (cross section)
Definition
Divides to top and bottom half
Term
Cranial
Definition
Towards head
Term
Rostral
Definition
Towards nose when referring to head
Term
Caudal
Definition
Towards tail
Term
Dorsal
Definition
Towards back
Term
Ventral
Definition
Towards belly
Term
Medial
Definition
Towards median plane
Term
Lateral
Definition
Away from median plane
Term
Deep
Definition
Towards center of body
Term
Superficial
Definition
Towards outside of body
Term
Proximal
Definition
Towards body on extremity
Term
Distal
Definition
Away from body on extremity
Term
Palmar
Definition
Back of forelimb distal to carpus
Term
Plantar
Definition
Back of hindlimb distal to tarsus
Term
Dorsal (on limb)
Definition
Front of fore/hind limb distal to carpus/tarsus
Term
Median plane
Definition
Saggital plane creating bilateral symmetry
Term
Dorsal Cavity
Definition
Central nervous system
Cranial cavity - brain
Vertebral cavity - spinal chord
Term
Ventral Cavity
Definition
Viscera of body
Thoracic - cranial to diaphragm- stomach, intestine, kidney, etc
Abdominal cavity - Caudal to diaphragm - stomach, intestines, kidney, etc
Pelvic cavity - ureters, reproductive organs, bladder, etc
Term
Visceral layer
Definition
Protective sack immediately surrounding an organ
Term
Parietal layer
Definition
Layer immediately within body wall
Term
Visceral layer of heart
Definition
Pericardial sack
Term
Space between visceral layer of heart and pleura
Definition
Pericardial space
Term
Fluid within pericardial space
Definition
Pericardial fluid
Term
Parietal layer of thoracic cavity
Definition
Pleura
Term
Space between visceral and pareital layers of thorasic cavity
Definition
Pleural space - filled with fluid
Term
Mediastinum
Definition
Area between left and right lung containing connective tissue, esophagus, trachea, thymus, heart, nerves, blood vessels etc.
Term
What is the benefit of having a complete mediastinum?
Definition
Allows each side of the thorasic cavity to operate separately. Ex: if one lung collapses, other can function independently.
Term
Do all animals have a complete mediastinum?
Definition
No
Term
Abdominopelvic cavity
Definition
Ventral cavity caudal to the diaphragm
Term
Parietal lining around whole abdominal cavity
Definition
Peritoneum
Term
Reflection of the paritoneum
Definition
Surrounds each organ to allow it to maintain location in abdominal cavity
Term
Omentum
Definition
Reflection of the paritoneum surrounding stomach and attaching to another visceral organ.
Term
Mesentery
Definition
Reflection of paritoneum that surrounds intestine and attaches to dorsal abdominal wall.
Term
Is water an organic or inorganic molecule?
Definition
Inorganic
Term
Is water polar?
Definition
Yes
Term
What percent of total body weight is made up of water?
Definition
60-70% depending on body condition of animal
Term
What fraction of body water is in intracellular fluid? Extracellular?
Definition
2/3 intracellular
1/3 extracellular
Term
What type of bond do water molecules form with eachother?
Definition
Hydrogen
Term
Cohesion
Definition
The tendency of water molecules to stick together
Term
What effect does the cohesion of water have in the body?
Definition
Allows blood to be transported through blood vessels because one water molecule is attracted to the next.
Term
How does water moderate the effect of temperature changes?
Definition
It takes more energy to break the hydrogen bonds between water molecules. It takes more energy to boil and freeze.
Term
What are the 4 important characteristics of water?
Definition
Cohesion, moderates temperature changes, extremely good solvent, forms H+ and OH- ions.
Term
Why is it significant that water has a very high heat of vaporization?
Definition
It takes a lot of energy to convert liquid water to vapor. In evaporative cooling - a lot of heat (energy) can be lost through perspiration as blood vessels at the body surface dilate and heat dissipates to sweat and is evaporated away.
Term
Why is it significant that water is an extremely good solvent?
Definition
Capable of dissolving a wide range of substances - most reactions in body take place between solutions. Can dissolve other polar molecules - sugars
Term
Solvent
Definition
More abundant substance in dissolved solution - does the dissolving ex: water
Term
Solute
Definition
In lesser amount - substance that is dissolved in solvent ex: salt
Term
Electrolytes
Definition
Ions dissolved in water that result in a solution that will conduct electricity.
Term
Hydrophilic
Definition
Molecules that can dissolve in water
Term
Hydrophobic
Definition
Non-polar molecules that don't dissolve in water ex: fats, oils, etc
Term
What effects can the ions H+ and OH- have?
Definition
Determines pH. If conc of H+ exceeds OH- = acidic. If conc of OH- exceeds H+ = basic. Pure water has equal concentration of H+ and OH- ions.
Term
What is the body pH of animals?
Definition
7.4
Term
What is the maximum change in body pH that an animal can tolerate?
Definition
.5
Term
What is the average and range for urine pH?
Definition
Average: 6, Range: 3-12
Term
Prokaryote
Definition
Organism containing cells without nuclei
Term
Eukaryote
Definition
Organism containing cells with nuclei in which DNA has combined with protein to form chromosomes - is surrounded by nuclear envelope. All animals are eukaryotes.
Term
Cell theory (4 rules)
Definition
1. Every living organism is made up of one or more cells
2. The smallest living organisms are unicellular.
3. Cells are the basic functional units of larger, multicellular organisms.
4. All cells arise from preexisting cells.
Term
What limits cell size?
Definition
Ability to perform basic cell functions.
Term
Which has more surface area in proportion to size: a small or large cell?
Definition
Small cell
Term
What is the average cell size in an animal?
Definition
10-30 micrometers
Term
Is it possible for a cell to have more than one nucleus?
Definition
Yes - some larger cells need larger nuclei to control cell functions.
Term
What is the largest cell type in the body?
Definition
Muscle fiber
Term
What does the nucleus do?
Definition
1. Facilitates cell division.
2. Contains DNA, RNA and protein
3. Control center for all metabolic functions.
Term
Is the nucleus membrane bound?
Definition
Yes - allows for movement of molecules in and out.
Term
What is the nucleolis? Where is it located? What function does it serve?
Definition
The nucleolis is a non-membrane bound organelle found within the nucleus where ribosomes are produced.
Term
Smooth endoplasmic reticulum
Definition
Is not studded with ribosomes. Site of lipid synthesis
Term
Rough endoplasmic reticulum
Definition
Studded with ribosomes - important in protein synthesis
Term
Is endoplasmic reticulum a single, double, or non membrane bound organelle?
Definition
Double
Term
Cysterna
Definition
Center of endoplasmic reticulum where proteins are temporarily stored.
Term
What is the function of a ribosome?
Definition
Protein synthesis
Term
Describe the path that synthesized proteins take.
Definition
1. Synthesized in ribosome.
2. Transported away from nucleus in cysterna.
3. Transport vesicles remove from cysterna and transport to Golgi apparatus.
4. Modified in Golgi apparatus then transported to final location by secretory vesicles.
Term
Where are the two locations where ribosomes can exist in the cell?
Definition
Rough endoplasmic reticulum and as free ribosomes in the cytoplasm
Term
Golgi apparatus
Definition
Site of carbohydrate manufacturing
Term
Exocytosis
Definition
Movement of material out of cell
Performed by secretory vesicles
Term
What is the difference between excretions and secretions?
Definition
Excretions are waste products, secretions are usable products produced by the body.
Term
Endocytosis
Definition
Movement of material into the cell.
Term
Phagocytosis
Definition
Endocytosis of solid material
Term
Pinocytosis
Definition
Liquid material into cell
Term
Receptor mediated endocytosis
Definition
Movement of material into cell that can only be facilitated by receptors.
Term
Lysosomes
Definition
Specialized vesicle formed by Golgi apparatus
Contain hydrolytic enzymes to break down nutrient molecules into useable units and digest intracellular debris.
Term
Peroxisomes
Definition
Like lysosmes but in organs that detoxify the body like the kidneys and liver.
Degrade harmful substances.
Term
Centrioles
Definition
Function in cell division
Term
Inclusions
Definition
Not true organelles - can be membrane bound, large or small.
Ex: Fat/adepose
Collection of metabolic (secretory)products that remain in cell.
Term
Mitochondria
Definition
"Power house" of cell - where most energy production within a cell occurs.
Term
Describe the anatomy of a mitochondria organelle.
Definition
Double membrane - inner membrane folded to increase surface area with enzymes for energy production.
Area within membrane filled with fluid called matrix.
Term
What types of materials are broken down in the mitochondria?
Definition
Carbohydrates, lipids and proteins
Term
Describe the process of cellular respiration
Definition
Oxygen gained from external respiration is used to extract energy from nutrients.
Carbon dioxide is given off as a waste product and exhaled through external respiration.
Term
What is ATP?
Definition
Adenosine triphospate - energy molecule that runs all functions of body needing energy.
Term
How is ATP produced?
Definition
Preferentially produced from glucose.
Gyoclysis (breakdown of glucose occurs), products enter citric acid/kreb's cycle and then electron transfer chain
Term
What is ATP used for?
Definition
Muscle contraction, membrane transport, protein synthesis.
Term
Vaults
Definition
Newly identified organelle present in all cells.
Play role in nuclear pores
Extremely small.
Term
What are the two major components of the cytoplasm?
Definition
Cytosol - semi-liquid gel material between nucleus and cell membrane
Cytoskeleton - skeletal structure holding organelles in place
Term
What are the major components of the cytoskeleton?
Definition
Microtubules, microfilaments and intermediate filaments
Term
Microtrabecular lattice
Definition
Combination of all skeletal structures in the cell
Term
Microtubules
Definition
Part of the cytoskeleton.
Facilitate the movement of organelles
Form centrioles
Form cilia and flagella
Made up of protein
Term
Cilia
Definition
From the latin "eyelash"
Short and numerous
Move in unison in undulating fashion
in lining of respiratory path and opening of ovaducts
Term
Flagella
Definition
From latin "whip"
Only one cell in mammals with single flagellum - sperm
Term
Microfilaments
Definition
Found in skeletal muscle - made of actin and miacin
Term
Intermediate filaments
Definition
Make up keratin in hair, nails and hooves
Term
3 major functions of cell membrane
Definition
Separates intra/extramolecular fluid
Functions and acts in communication with other cells
Selectively permeable barrier
Term
What is the cell membrane made of?
Definition
Phospholipids and proteins in a phospholipid bi-layer.
Term
What is a phospholipid?
Definition
Triglyceride where one fatty acid has a phosphate group replacement making it hydrophilic
Term
What aspect of a phospholipid is hydrophilic and what is hydrophobic?
Definition
Fatty acid tails are hydrophobic and point towards center, trigylceride head is hydrophilic and points towards intra/extracellular fluid.
Term
What is embedded in the cell membrane?
Definition
Proteins - some are one one side or the other, some go all the way through.
Term
What percent of the membrane is made up of proteins?
Definition
About 50%
Term
What do some proteins in the cell membrane have attached to them and what does this allow them to do?
Definition
Carbohydrates
Cell adhesion molecules - allows cell to cell contact
Facilitate movement of molecules in/out of cell
Term
Concentration in fluid
Definition
Number of molecules/atoms dissolved in fluid
Term
Gradient
Definition
Physical difference between two regions in the concentration of molecules
Term
In what direction do molecules typically move between cells/ through membranes?
Definition
From areas of high concentration to low concentration.
Term
Passive transport
Definition
The movement of molecules across a cell membrane without the use of energy.
Term
Diffusion
Definition
Passive transport of molecules across a cell membrane
Term
What three factors determine how readily molecules move across a cell membrane?
Definition
Molecular size - large diffuse slower than small
Lipid solubility - lipid soluble move quickly
Molecular charge - charged atoms do not move quickly
Term
What will happen if a gradient occurs but the molecule in question cannot permeate the membrane?
Definition
Water will move across instead from low concentration to high.
Term
Osmosis
Definition
Passive movement of water through a semi-permeable membrane down a concentration gradient
Term
What is the force that moves water across a membrane?
Definition
Osmotic pressure
Term
What molecules other than water are free to passively move through a membrane?
Definition
Small, uncharged and non-polar molecules.
Term
Isotonic
Definition
Equal concentration of molecules in and out of a cell
Term
Hypertonic
Definition
Increased concentration of molecules outside a cell.
Water leaves cell and causes it to shrivel up.
Term
Cremation
Definition
When a cell shrivels up and is no longer able to move through blood vessels well
Term
Hypotonic
Definition
Lower concentration of molecules outside cell
Water moves into cell to even out concentration
Term
Hemolysis
Definition
Bursting of red blood cells.
Term
How do ions move across a cell membrane?
Definition
Passive transport
Move along electrical gradient to even out charge
Term
Facilitated diffusion
Definition
Passive transport
Movement of molecules that cannot diffuse without assistance
Certain carrier proteins will only accept certain molecules
For molecules too large to diffuse on their own.
Term
Active transport
Definition
Not passive - requires energy
Move uphill against concentration gradient
Uses transmembrane proteins
Term
Is endo/exocytosis passive or active transport?
Definition
Active
Term
Membrane potential
Definition
Uneven distribution of ions on either side of a cell membrane
Causes one side to be positive and one to be negative
Creates potential to do work.
Term
Connective tissue functions
Definition
1. Form metabolic and structural connections between tissues
2. Protective sheath around organs
3. Stores fat
4. Bone- provides structural frame for body
5. Blood - transports substances throughout body
6. White blood cells - Healing process and control invading microorganisms
Term
Components of connective tissue (2)
Definition
Extracellular matrix and ground substance
Term
Where is the extracellular matrix of the connective tissue located
Definition
Outside the cell
Term
What is the extracellular matrix of connective tissues made of?
Definition
Extracellular fibers
Term
What are the three types of extracellular fibers in connective tissue?
Definition
Collagenous fibers
Reticular fibers
Elastic fibers
Term
Collagenous fibers
Definition
Tendons and ligaments
Made of collagen
Gives strength to connective tissue
Arranged in long, parallel rows
White
Term
Reticular fibers
Definition
Surrounds and supports highly cellular organs and blood vessels/nerves
Made of thin, delicate branched network of collagen proteins
Term
Elastic fibers
Definition
Vocal chords, lungs, skin
Made of elastic protein
Yellow
Not as strong as collagen
Acts as rubber band, stretches and returns to shape
Term
Ground substance
Definition
Liquid, gel, or solid
Acts as shock absorber, protects cells, medium of transport
Term
Fixed cells
Definition
Manufacture and excrete ground substance
Each type of connective tissue has own fixed cell type
Transient - come and go as needed
INvolved in repair and protection of tissues
Leukocytes, mast cells macrophages
Term
Fixed cell types
Definition
Fibroblasts (type of cell that all other fixed types are from), chondroblasts, osteoblasts, adipocytes, reticular cells
Term
2 connective tissue categories
Definition
Connective tissue proper and Specialized connective tissue
Term
Connective tissue proper
Definition
Binds other tissues together, provides framework for organs
Types: Loose and Dense
Term
Loose connective tissue types
Definition
Areolar, Adipose, reticular
Term
Areolar
Definition
Most common type of connective tissue
Scattered network of fibroblasts, loose network of collagen, elastic and reticular fibers
Holds tissues together, supports internal organs and envelopes blood vessels and nerves.
Term
Adipose
Definition
Fat - Stores excess nutrients, acts as shock absorber, insulates
White - adipocytes - very little intercellular material other than fat
Brown - in neonates and hibernating animals. Thermoregualation - lots of mitochondria - produce heat
Term
Reticular
Definition
Found in organs that are highly cellular - spleen, lymph nodes, liver
Forms stroma - framework of organs
Term
Dense connective tissue types
Definition
Dense regular, dense irregular, elastic
Term
Dense regular CT
Definition
parallel rows of collagenous fibers
white/silver
few blood vessels - slow to heal
tendons, ligaments, and fascia (connects muscles to bones)
Term
Dense irregular CT
Definition
Irregularly aligned cells
Dermis covering organs
Term
Elastic
Definition
Elastic fibers - most flexible
In intervertebral disks and certain ligaments
Yellow
Term
Ligamentum nuchae
Definition
type of elastic ligament found in browsing animals from back of head down neck to withers
allows to graze effectively without using muscles as much
Term
Specialized Connective Tissue
Definition
Can either be liquid or soid
Cartilage, bone and blood only
Term
Cartilage
Definition
Lines joints of body, pinnae, larynx, tracheal rings, embryonic skeleton
Made up of chondrocytes - found in lacuna
Matrix: elastic and collagen fibers
Hyaline, fibrocartilage and elastic cartilage
Term
Hyaline Cartilage
Definition
Most common
Ends of long bonds at articulation (joint)
Connects ribs to sternum
Tracheal rings and embryonic skeleton
Term
Elastic Cartilage
Definition
More stretchy because of elastic fibers
Pinnae, epiglottis, tip of nose
Term
Fibrocartilage
Definition
Lots of collagen fibers
Meniscal, intervertebral disks, pubic synthesis
Term
Bone
Definition
Most rigid tissue in body
Made up of osteocytes - in lacunae
Secrete own matrix with lots of calcium to form regidity
Term
Blood
Definition
Matrix of blood - plasma
Red blood cells - erythrocytes
White blood cells
Collagen and elastic fibers loosely arranged within vessels, come together to form clots
Term
Muscle tissue
Definition
Composed of actin and myosin
Called muscle cells, fibers or myocytes
Function: to contract
3 types: Skeletal, smooth, cardiac
Term
Skeletal muscle
Definition
Striated muscle
Long cylindrical cells running entire length of muscle
Mutlinucleate cells
Voluntary muscle
contracts rapidly
Term
Smooth muscle
Definition
Lines hollow, contracting organs - esophagus, stomach, intestines, urinary bladder, ureters, blood vessels
Cells taper at both ends
Not regularly arranged
No striations
Involuntary control, uninucleate
Slower contraction rate than skeletal muscle
Reponsible for peristalsis
Term
Cardiac muscle
Definition
Only in hart
Striated, uninucleate,cells branching shape
Cells connected by intercalated disks - specialized junctions allowing impulses to be transmitted
Involuntary control
Term
Nervous tissue
Definition
Found in brain, spinal cord, peripheral nerves
Neuron and neuroglial cells
Term
Neuron
Definition
Specialized to send electrical impulses
Unable to divide
can be longest cells in body
3 parts: Perikaryon - cell body
dendrites - multiple short extensions to receive impulses
Axons - long, singular extensoin to send impulses
Axons synapse with other cells to make functions happen.
Term
Neuroglial/Glial Cells
Definition
Support and protect neurons
Can divide
Term
Epithelial tissues
Definition
Line other tissues of body
Single or multiple layers thick
Avascular - lack blood supply - rely on underlying connective tissue for nutrients
Innervated - have nerve supply
Term
Function of epithelial tissue:
Definition
Absorb, secrete and excrete biochemical substances
Play role in sensory reception - sensory nerve endings in epithelial cells
Term
Epithelial cell parts
Definition
Apical surface - faces lumen or outside of organ
Basal surface - Faces underlying connective tissue, basal lamina, or blood vessels
Term
Basal lamina
Definition
Basement membrane - non-cellular structure made of protein fibers attaching basal surface of epithelial cells ot underlying connective tissues
Term
Lumen
Definition
In tubular/round organs - interior
Term
Epithelial cell junction types
Definition
Tight, gap and desmosomes
Term
Tight cell junction
Definition
Protein complexes join plasma membranes together
very secure seal
in tissues that should not leak - urinary bladder, digestive tract
Term
Gap cell junction
Definition
Tubular channel proteins between two cell membranes
Allow exchange and passage of ions, nutrients, nerve impulses, water
Found in tissues contracting and moving in unison - heart and smooth muscle tissue
Term
Desmosome cell junction
Definition
Square protein with filaments into nearby cells
Tissues that undergo repeated episodes of tension and stretching
Attach basal surface to basal lamina
Term
Epithelial surface specialization
Definition
Smooth
Microvilli (brush border)-smaller than cilia, projections of membrane surface. In small intestines, lining of kidney. for absorption of materials - increases surface area.
Cilia
Keratin
Term
Classification of epithelial tissue
Definition
Number of layers: simple or stratified
Shape of cells: Squamous (flattened), Cuboidal (square), Columnar (rectangular)
Term
Anatomy
Definition
Study of structure of body parts
Term
Physiology
Definition
Study of function of body parts
Term
Gross anatomy
Definition
Macroscopic
Term
Microscopic anatomy
Definition
Can only see with microscope
Term
Cytology
Definition
Looking at blood under microscope
Term
Histology
Definition
Looking at tissue under microscope
Term
Homeostasis
Definition
Keeps all body systems balanced
Concentration of: nutrients, O2, CO2, waste products, water, salts, other electrolytes, Temperature reg, blood pH, white blood cell count, volume, pressure
Term
Characteristics of living things
Definition
Acquire and use materials and energy
Maintain homeostasis
Grow (in size or cell replacement)
Respond to stimuli
Reproduce (for pop health)
Evolve (for species health)
Term
Levels of organization
Definition
Chemical/Molecular
Cellular
Tissue
Organs
Organ systems
Organism
Term
Cell functions
Definition
Obtain nutrients and O2 for Energy
Perform chem reactions - simple units to energy
Synth proteins and cell components
Respond to stimuli
Control exchange of materials
Move materials
Reproduce
Term
Tissue types
Definition
Muscle
Nervous
Connective
Epithelial
Term
Extra cellular fluid
Definition
Interstatial (tissue) fluid + Plasma
Term
Pathophysiology
Definition
Abnormal functioning of body during disease
Term
Transitional epithelium
Definition
Organs that stretch and hold form
When empty - round/cuboidal
When full - squamosal
Term
Glands
Definition
Derivatives of epithelial tissues
Term
Exocrine glands
Definition
Excrete products to ducts
Term
Endocrine
Definition
Excrete hormones into circulatory system
Term
Merocrine Glands
Definition
Excrete products through exocytosis
Term
Apocrine Glands
Definition
Store secretions in vesicles in top part of cell then release top section
Term
Holocrine
Definition
Build up secretion in cells then release entire cell
Term
Mucous membranes
Definition
Mucosae - line all cavities that open to outside
Term
Goblet cells
Definition
Unicellular exocrine gland
Excrete mucus
Term
Serous membranes
Definition
Epithelial linings in body cavities
Secrete watery secretions
Parietal and visceral layers
Term
Synovial membranes
Definition
Line articulations of long bonds
Acts as joint lubricant
Term
Cutaneous membrane
Definition
Skin
Term
Integumentary System
Definition
Skin and related structures
Functions: Thermoregulation, Sensory perception, synthesis of vit D
Term
Adnexae
Definition
Related structures to skin
Term
Epidermis
Definition
Upper most layer of skin
Lacks blood vessels and direct nerve supplies
Derives all nutrients from dermis
Term
Keratinocytes
Definition
Epidermal cell that produces keratin - waterproofs skin
Term
Melanocytes
Definition
Epidermal cells - Produce melanin pigment
Term
Merkel cells
Definition
Immune cell in epidermis to phagocytize bacteria
Term
Langerhans cells
Definition
Epidermal immune cell involved in allergic reactions
Term
Stratum Basale
Definition
Basal layer of epidermis
Deepest layer
Single layer of living keratocytes
Undergo mitosis - older cells move superficially
Find merkel cells, melanocytes, and keratocytes
Term
Stratum Spinosum
Definition
Superficial to stratum basale
Several layers of keratocytes
finder langerhans cells
Term
Stratum granulosum
Definition
Granular layer
superficial to stratum spinosum
Cells filled with keratin that exudes into surrounding tissue
First layer to have waterproofing function
Term
Stratum Lucidum
Definition
Clear layer
Only found in very thick skinned areas
Foot pads, nose pads, sometimes elbows, knees
Term
Stratum corneum
Definition
Filled with keratin
20-30 layers of dead epithelial cells
Term
In fully furred animals, what epidermis layers are present?
Definition
Stratum corneum, stratum spinosum stratum basale
Term
Epidermal Papilla
Definition
Knoblike elevations where whiskers and other tactile hairs arise
Term
Dermis
Definition
Corium
Deep to epidermis
Dense irregular connective tissue - collagen, elastic, reticular fibers
Find hair follicles, nerve endings, glands, smooth muscle, blood vessels, and lymphatics
Has Fibroblasts, adipocytes and macrphages
Term
Papillary layer
Definition
Layer of epidermis
Dermap papillae - surface like egg carton - cements dermis and epidermis together
Find blood vessels, pain and touch receptors
Term
Reticular layer
Definition
Indistinct boundary with papillary layer
Term
Hypodermis
Definition
Deep to dermis
Connects skin to underlying tissue
Composed of loose areolar tissues
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