Term
| What are the assumptions of Michaelis-Menten Enzyme Kinetics? |
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Definition
1. The reaction has only one substrate
2. The molar concentration of the substate is much higher then that of the enzyme
3. Only the initial reaction rate is considered
4. Short time period of observation |
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Term
| How do the kinetics of a first order reaction work? |
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Definition
| reaction rate is directly proportional to substrate concentration |
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Term
| How do the kinetics of a second order reaction work? |
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Definition
| reaction rate is proportional to the concentration of both substrates |
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Term
| How do the kinetics of a zero order reaction work? |
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Definition
| This is a reaction solely dependent on efficiency and concentration of the enzyme |
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Term
| How many k values exist of Michaelis-Menten Enzyme Kinetics? |
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Definition
3
2 first order
1 second order |
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Term
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Definition
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Term
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Definition
| maximum reaction velocity, is approached at a saturating [S] |
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Term
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Definition
turnover number or catalytic rate constant
-essentially how efficiently the enzyme converts substrate after binding |
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Term
| What is the v of a reaction given [S] much lower then Km? |
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Definition
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Term
| What is the v given [S] = Km? |
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Definition
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Term
| What is the v given [S] much higher then Km? |
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Definition
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Term
| What is the specificity constant? |
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Definition
Kcat/Km
describes the efficiency of an enzyme. |
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Term
| Why do most enzymes in a living cell work with substrate concentrations below Km? |
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Definition
| At that level the enzyme is much more modifiable, allowing for fine tuning |
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Term
| What are the axis for a lineweaver burk plot? |
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Definition
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Term
| What are the axis in a Eadie-Hofstee plot? |
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Definition
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Term
| What are the axis in a Hanes-Wolf plot? |
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Definition
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Term
| What is the y intercept in a Lineweaver-Burk plot? |
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Definition
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Term
| What is the X intercept in a lineweaver burk plot? |
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Definition
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Term
| What is the slope in a lineweaver burk plot? |
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Definition
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Term
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Definition
| In first order kinetics it is the time period it takes for half of substrate to be consumed |
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Term
| First order reactions have a changing or stable half-life? |
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Definition
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Term
| Zero-order reactions have a constant or changing half life? |
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Definition
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Term
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Definition
| The factor by which a reaction is accelerated given a 10 degree (Celsius) rise in temperature |
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Term
| Why are enzymatic Q10's lower then non-catalyzed reactions? |
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Definition
| At high temperatures enzymes denature, stalling reaction speeds |
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Term
| How does Ph affect enzymes? |
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Definition
| Depends, each enzyme has its own critical range |
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Term
| How do coenzymes interact with enzymes? |
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Definition
Via non-covalent linkages.
They operate as substrate in two-substrate reactions.
Reconverted after reaction |
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Term
| How do prosthetic factors work with enzymes? |
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Definition
| Covalently bond to form part of the active center |
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Term
| What are the coenzymes of dehydrogenase reactions? |
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Definition
NAD and NADP
acquire two electrons and a proton during catabolic reactions
Some use FAD and FMN |
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Term
| Which coenzyme activates organic acids? |
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Definition
CoEnzyme A CoA.
soluble carrier of acyl groups |
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Term
| What is the function of S-Adenosyl methionine (SAM)? |
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Definition
| Coenzyme that donates methyl groups |
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