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(Enterobacteriaceae) TSI Agar
N/a
8
Microbiology
Undergraduate 3
04/09/2015

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Term
TSI
Definition
Triple sugar iron which includes 3 sugars: Glucose, lactose and sucrose. The concentrations of lactose and sucrose are 10x higher than that of glucose.
Starts out red and turns red when acidic
Term
What do Enterobacteriaceae use first in TSI?
Definition
Enterobacteriaceae will use glucose first and then either die or use sucrose and lactose.
If it can't use the sugars it will break down peptose under aerobic conditions and it will revert back to neutral pH
Term
What does black precipitate in the butt of TSI mean?
Definition
The organism has used the iron
Term
How do you record the results of a TSI test?
Definition
Recored: Slant/Butt
Term
Yellow/Yellow (Acid/Acid)
Definition
Positive for lactose/and or sucrose. Positive for glucose in the butt.
H2S: Negative
Term
Red/Yellow (Alkaline/Acid)
Definition
Positive for glucose lactose.
Negative for lactose and sucrose
H2S: Negative
Term
Yellow/Black (Acid/H2S)
Definition
Lactose and or sucrose positive
Glucose: Positie
H2S: Positive
Term
Red/Black (Alkaline/H2S)
Definition
Lactose and sucrose: Negative
Glucose: Negative
H2S: Negative
(Indicates the the organism is not a member of the Enterobacteriaceae
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