Term
| What organisms are always considered pathogenic in stools? |
|
Definition
E. coli O 157:H7
Salmonella spp
Shigella spp
Yersinia spp |
|
|
Term
| General characteristics of Enterobacteriaceae |
|
Definition
Gram neg bacilli, bipolar (safety pin) staining
All ferment glucose
All reduce nitrate
All are oxidase negative
All are catalse positive |
|
|
Term
| Growth requirements for Enterobacteriaceae |
|
Definition
Facultative anaerobes
Not fastidious
Grow on MAC (Pink means they ferment lactose) |
|
|
Term
| Enterobacteriaceae colonies on BAP |
|
Definition
| Large, gray, dry or mucoid. Hemolysis is variable |
|
|
Term
| Tribe Proteeae general characteristics |
|
Definition
PAD positive (*Only Enterobacteriaceae that is pos)
Motile
ONPG neg
Methyl red pos/ VP neg |
|
|
Term
|
Definition
Soil/water
NF of human stoools |
|
|
Term
| Proteus can be pathogenic under what conditions? |
|
Definition
Patient has"
UTI
Wound infection
Respiratory tract infection
If they are found in blood |
|
|
Term
| Colonial morphology of Proteus |
|
Definition
BAP: Will swarm (appearing as waves)
MAC: Clear, lactose neg colonies
XLD: Yellow colonies with black centers
MTM: No growth or small, pinpoint colonies |
|
|
Term
| Key biochemical characteristics of Proteus |
|
Definition
PAD: Postive
H2S: Positive
Geltain (at 22 degrees Celsius): Positive |
|
|
Term
| What are the clinically significant species of Proteus? |
|
Definition
Proteus mirabilis
Proteus vulgaris
Proteus penneri |
|
|
Term
|
Definition
Most frequently encountered Proteus.
Seen mostly in UTI's (can be seen in nosocomial infections and pneumonia)
Very sensitive to most antiobiotics |
|
|
Term
| Proteus mirabilis key biochemicals |
|
Definition
Indole: Negative
Ornithine decarboxylase: Positive |
|
|
Term
|
Definition
Causes UTI's and wound infections
Resistant to penicillin and 1st generation cephalosporins |
|
|
Term
| Proteus vulgaris key biochemicals |
|
Definition
Indole: Positive
Ornithine decarboxylase: Negative |
|
|
Term
|
Definition
Infrequent isolate; seen in ICP's.
Resistant to chloramphenicol |
|
|
Term
| Proteus penneri key biochemicals |
|
Definition
Indole: Negative
Ornithine decarboxylase: Positive |
|
|
Term
| Minimum criteria for speciating Proteus spp. |
|
Definition
Swarming on BAP-> Proteus spp.
Spot indole positive-> Proteus vulgaris
Spot indole negative-> Perform ornithine decarboxylase.
Ornithine decarboxylase Positive-> Proteus mirabilis
Ornithine decarboxylase Negative-> Proteus penneri |
|
|
Term
| Morganella spp. of the Tribe Proteeae facts |
|
Definition
NF of stools
Causes UTI's, respiratory and wound infections |
|
|
Term
| Colonial morphology of Morganella spp. |
|
Definition
BAP: Non-swarming
MAC: Clear, white colonies (lactose neg)
XLD: Clear colonies |
|
|
Term
| Key biochemicals for Morganella spp. |
|
Definition
PAD: Positive
H2S: Negative
Gelatin (at 22 degrees Celsius): Negative
Ornithine decarboxylase: Positive
Citrate: Negative |
|
|
Term
| Quickly differentiate between Proteus and Morganells |
|
Definition
Morganella do not swarm while Proteus does.
Morganella do not liquefy gelatin while Proteus does
Morganella is not positive for H2S while Proteus is. |
|
|
Term
|
Definition
NF of stools
Can cause UTI's in pateints with underlying urologic disorders |
|
|
Term
| Key biochemicals of Provendencia spp. |
|
Definition
PAD: Positive
H2S: Negative
Gelatin liquefaction (at 22 degrees Celsius)
Ornithine decarboxylase: Negative
Citrate: Negative |
|
|
Term
| Quickly differentiate between Provendencia spp. and Morganella spp. |
|
Definition
Provendencia is positive for citrate while Morganella is not.
Provedencia is negative for ornithine decarboxylase while Morganella is positive |
|
|
Term
| Clinically significant species of Provendencia spp. |
|
Definition
P. stuartii
P. rettgeri
P. alcalifaciens |
|
|
Term
| Speciation of Providencia spp. is performed how? |
|
Definition
| The use of the adonitol, inositol and urea tests |
|
|
Term
|
Definition
Adonitol: Positive
Inositol: Positive
Urea: Positive |
|
|
Term
|
Definition
Adonitol: Negative
Inositol: Positive
Urea: Negative |
|
|
Term
| P. alcalifaciens reactions |
|
Definition
Adonitol: Positive
Inositol: Negative
Urea: Negative |
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