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Microbiology Gram- Mac+ Ox- Bacilli
Enterobacteriaceae, Acinetobacter and Stenophomonas
58
Microbiology
Graduate
11/25/2012

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Cards

Term
Three common characteristics of enterbacteriaceae
Definition
glucose fermentation, reduces nitrate to nitrite and oxidase negative
Term
Methyl Red test
Definition
detects the production of mixed acids as a result of subsequent metabolism of pyruvate
Term
Voges-Proskauer test
Definition
detects production of acetonin as a result of subsequent metabolism of KOH and naphthol reagents
Term
acid slant and acid butt on TSI agar
Definition
able to ferment which lactose and sucrose
Term
enterotoxigenic E.coli
Definition
travelers disease
Term
K. pneumoniae
Definition
often associated with lobar pneumoniae in elderly hospitalized patients
Term
Acquired by eating improperly cooked or preserved contaminated food, what produces dysentery in affected individuals
Definition
Shigella
Term
The identifying characteristics of Acinetobacter
Definition
gram negative bacilli, NLF, non glucose fermenters, oxidase negative
Term
Carriers of salmonella harbor the organism
Definition
in their small intestine
Term
A clear red colony with a black center on XLD agar
Definition
is salmonella
Term
The test that can differentiate P. mirabilis from P. vulgaris is
Definition
indole test, P. vulgaris is positive and P. mirabilis is negative
Term
The optimal temperature for growth of Yersinia is
Definition
25
Term
K. oxytoca can be differentiated from K. pneumonia
Definition
by an indole test, K. oxytoca is indole positive
Term
Because Acinetobacter tend to resist decolorization they can be mistaken for
Definition
Neisseria
Term
Fermentation
Definition
anaerobic metabolism
Term
oxidative metabolism
Definition
respiration with O2 as final electron acceptor
Term
saccharolytic
Definition
utilizes glucose
Term
asaccharolytic
Definition
does not utilize glucose
Term
The 4 virulence factors of enterbacteriaceae
Definition
Endotoxin, Specific toxins, Capsules and Adherence factors
Term
6 sites of enterbacteriaceae infections?
Definition
blood, CNS, GI tract, Lower respiratory, UTI, wounds
Term
GI tract infections can cause 5 things...
Definition
diarrhea, dysentery, hemorragic colitis, hemolytic uremic syndrome, and typhoid fever
Term
Lower respiratory infections can cause
Definition
plaque
Term
Serotype O is associated with
Definition
cell wall
Term
serotype H is associated with
Definition
flagella
Term
serotype K is associated with
Definition
capsules
Term
3 forms of treatment
Definition
Aminoglycodies, Trimethoprim-sulfamethoxazole and 3rd generation cephalosporins
Term
resistant to ampicillin and carbenicillin
Definition
Klebsiella and Citrobacter
Term
resistant to ampicillin and cephalothin
Definition
Enterobacter and Hafnia
Term
resistant to colistin
Definition
Proteus, Morganella and Serratia
Term
resistant to multiple drugs
Definition
Providencia and Serratia
Term
MacConkey agar
Definition
detects lactose fermentors
Term
Hektoen, XLD, SS agar
Definition
detects Salmonella and Shigella
Term
Gram-negative, selenite broth
Definition
enrichment for salmonella and Shigella
Term
CIN
Definition
selective for yersinia
Term
MacConkey Sorbitol
Definition
differential for E.coli O157
Term
TSI Slant
Definition
Lactose, sucrose or glucose fermentation, H2S production and gas production
Term
Indole test
Definition
detects production of indole as a result of subsequent metabolism of tryptophan
Red/pink = +
Term
Citrate Test
Definition
detects production of citrate as a result of subsequent metabolism of carbon and ammoninium as nitrogen source

Blue = +
Term
Decarboxylases test
Definition
detects hydrolysis of amino acids via pH change
Term
ONPG test
Definition
detects presence of beta galactosidase
yellow = +
Term
Phenylalanine deaminase
Definition
detects production of phenylpyruvic acid as a result of subsequent deamination of phenylalanine

green = +
Term
Urease test
Definition
detects production of ammonia and CO2 as a result of hydolysis of ura

pink = +
Term
Nitrate test
Definition
detects production of nitrite

red = +... add zinc then colorless = +
Term
Three commerical ID kits
Definition
API 20E, Vitek, and Microscan
Term
Dieases that E.coli can cause
Definition
UTI and bacteremia
Term
ETEC
Definition
EnteroToxigenic E. Coli- travelers and childhood diarrhea, profuse watery stools
Term
EIEC
Definition
EnteroInvasive E. Coli- dysentery, necrosis, ulcers and inflammation
Term
EPEC
Definition
EnteroPathogenic E. Coli- chronic diarrhea
Term
EHEC
Definition
EnteroHemorrhagic E. Coli- inflammation and bleeding of large bowel, hemolytic uremic syndrome
Term
EAEC
Definition
EnteroAggregative E. Coli- prolonged watery diarrhea
Term
Lactose Fermenters...
Definition
E.coli, Klebsiella
Term
Nonlactose fermenters
Definition
Salmonella, Shigella, Proteus and Morganella
Term
defining characteristics of E.coli
Definition
ONPG positive, indole positive, associated with petting zoos
Term
defining characteristics of Shigella
Definition
causes dysentary, non motile, methyl red positive
Term
Defining characteristics of Salmonella
Definition
associated with poultry, three categories of disease (one being tyhpoid fever), black centers on XLD, urease negative
Term
defining characteristics of Klebsiella
Definition
causes the plaque, indole negative (except for k. oxytoca), non motile
Term
defining characteristics of proteus
Definition
swarming on plates, urease positive
Term
defining characteristics of morganella
Definition
urease positive
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