Term
| The atmospheric condition required by N. gonorrhoeae is: |
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Definition
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Term
A gram-negative diplococcus isolated from a CSF specimen gives the following results:
MTM - growth
Nutrient agar - No growth
Oxidase - Positive
Carbohydrate utilization tests: glucose - yellow, maltose-yellow, lactose-red, sucose-red
This is organism: |
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Definition
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Term
| The organisms in a gram-stained smear prepared from an 18-hour-old culture of Neisseria spp. appear blue on microscope examination. These organisms are: |
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Definition
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Term
An oxidase-positive, gram negative diplococcus growing on CHOC agar gives the following reactions:
Beta-Galactosidase - negative
Gamma-Glutamylaminopeptidase - negative
Hydroxyprolylaminopeptidase - positive
The technologist should: |
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Definition
| Subculture the isolate onto a gonococcal selective medium |
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Term
| When a gram-stained smear for N. gonorrhoeae is requested on a throat specimen, the laboratory should: |
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Definition
| Notify the requester that the specimen is not appropriate for the test request and suggest a culture instead |
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Term
A technologist performs an oxidase test on an isolate of Neisseria spp. using an oxidase reagent ampule opened the previous day. A purple color appears 45 seconds after the organism is rubbed with an applicator stick onto filter paper saturated with reagent.
The technologist should: |
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Definition
| Repeat the test using an oxidase reagent ampule opened the day the test is performed |
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Term
| The reagent for the superoxol test is: |
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Definition
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Term
Which of the following holding or transportation methods is NOT appropriate for N. gonorrhoeae?
A. inoculated plates in candle jar @ 35
B. Nutritive transport system @ 35
C. Swabs in nonnutritive transport mediate @ RT
D. Swabs in nonnutritive transport media under refrigeration |
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Definition
| D. Swabs in nonnutritive transport media under refrigeration |
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Term
Which of the following media is NOT selective for N. meningitidis?
A. CHOC
B. ML
C. NYC
D. MTM
E. GCL |
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Definition
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Term
| The best specimen for diagnosing gonorrhea by gram-stained smear is: |
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Definition
| Urethral discharge from a man |
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Term
Which of the following combinations of media is the best for culturing join fluid when N. gonorrhoeae is suspected?
A. blood and MTM
B. blood and EMB
C. ML & MTM
D. blood & CHOC |
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Definition
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Term
A gram-negative diplococcus was isolated from the endocervical culture of a 10-year-old girl. The organism gave the following test results:
MTM - growth
Oxidase - Positive
Carbohydrate Utilization Tests: glucose-positive, maltose-negative, lactos-negative, sucrose-negative
The technologist should: |
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Definition
| Confirm the identity of the isolate with another method |
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Term
| A technologist is performing a nitrate reduction test. When dimethyl-alpha-naphthylamine and sulfanilic acid were added, the medium did not change color. The technologist should: |
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Definition
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Term
True or False:
Gonococci often have two types of colonies |
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Definition
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Term
True or False:
The penicillin disk test helps to distinguish M. catarrhalis from Neisseria species |
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Definition
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Term
| V factor is another name for: |
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Definition
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Term
Identification tests performed on a Haemophilus isolate gave the following results:
Beta hemolysis
Growth between the X & V factor strips
This organism is: |
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Definition
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Term
A Haemophilus isolate gave the following reactions:
Porphyrin test: fluorescence
Satellites around Staph. aureus
No hemolysis |
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Definition
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Term
| The purpose for the Hib vaccine is to protect children from infections caused by: |
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Definition
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Term
| A gram-negative rod is isolated from the blood culture of an 18-month-old child. The organism gorws on CHOC but not blood or eosin-methylene blue agar. The best test to perform on this isolate is: |
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Definition
| X & V factor requirements |
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Term
| A medium appropriate for the satellite test is: |
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Definition
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Term
An X & V factor requirement test was performed on a Haemophilus isolate in the following manner:
1. suspension of organism was prepared in TSB
2. Organism swabbed onto CHOC
3. X factor and V factor strips placed on agar plate
4. Plate was incubated for 24 hours at 35 degrees Celsius in CO2
5. Plate showed growth over entire plate
An evaluation of the procedure and test results indicates that the: |
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Definition
| test is invalid; CHOC agar is NOT appropriate for the X and V factor requirement test |
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Term
| A gram stain is prepared from an 18-hour-old culture of Haemophilus. The organisms appear blue on microscopic examination. These organisms are: |
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Definition
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Term
| A species of Haemophilus that requires X factor only is: |
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Definition
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Term
True or False:
Chancres are caused by H. dycreyi |
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Definition
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Term
True or False:
H. influenzae biogroup aegyptius causes "pink eye" |
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Definition
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Term
True or False:
The "school-of-fish" gram stain morphology is characteristic of H. parainfluenzae |
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Definition
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Term
True or False:
H. influenzae is part of the normal flora of the human upper respiratory tract |
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Definition
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Term
| Enteric "H" antigens are located in the: |
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Definition
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Term
| Enteric "O" antigens are located in the: |
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Definition
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Term
| Enteric "K" antigens are located in the: |
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Definition
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Term
| Gram-negative broth enriches for: |
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Definition
| Salmonella and Shigella species |
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Term
An organism that produces biochemical reactions consistent with Shigella sonnei does NOT agglutinate in the following Shigella antisera:
Serogroup A, B, C, or D
The technologist should: |
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Definition
| Boil the organism and repeat the serogrouping procedure |
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Term
| A TSI is inoculated on a Monday. On Wednesday, the TSI is completely red. The technologist should: |
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Definition
| Inoculate a fresh TSI tube and incubate for 18-24 hours |
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Term
| The best combination of organisms to use when performing QC on TSI is: |
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Definition
E. coli
Salmonella
Shigella
Pseudomonas species |
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Term
| An organism produces colorless colonies with black centers on SS agar. The expected appearance of this organism on MAC agar is: |
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Definition
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Term
| The best combination of organisms to use when performing QC on XLD agar is: |
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Definition
E. coli
Salmonella
Shigella
Enterococcus species |
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Term
| A K/A reaction on KIA indicates that the organism is a : |
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Definition
| Glucose fermenter: lactose nonfermenter |
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Term
| The organism that most often causes UTIs: |
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Definition
| Gives a TSI reaction of A/A |
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Term
The blood cultures from a patient who underwent an appendectomy grew a gram-negative bacillus. The organism gave the following results:
TSI: A/A
KIA: K/A
Urea: Positive
Motility: + @ 25, = @ 35
The results are consistent with: |
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Definition
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Term
An organism isolated from a stool culture gives the following reactions:
TSI: Red/Black
VP: Colorless
Lysine: Purple, Control tube: yellow
Urea: Yellow
Indole: Colorless
Phenylalanine: Colorless
Citrate: Blue
The technologist should now: |
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Definition
| Perform a serogrouping test with Salmonella antisera |
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Term
| A patient is diagnosed with HUS. A stool is submitted for culture. What medium should be included to detect the organism associated with HUS? |
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Definition
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Term
|
Definition
| Beta-galactosidase activity |
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Term
An organism isolated from a wound specimen gives the following reactions:
TSI: A/A
VP: +
Phenylalanine: =
Motility: +
Urea: =
What are the best set of tests to perform to identify this organism? |
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Definition
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Term
Which organism-colonial morphology pair is Incorrect:
E.coli - green metallic sheen on EMB
Y. enterocolitica - bull's eyes on CIN
Serratia marcescens - pink pigment
Providencia spp. - swarming
K. pneumoniae - mucoid colonies |
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Definition
| Providencia spp. - swarming |
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Term
| Which media does NOT contain a sulfur source AND an H2S indicator? |
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Definition
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Term
A stool isolate gives the following reactions:
TSI: K/A with H2S
LIA: R/A
Urease: Positive
These results are consistent with which organism? |
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Definition
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Term
| Organisms that typically produce clear colonies (green without black centers) on Hektoen agar are? |
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Definition
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Term
True or False:
The spot indole and tube indole tests use the same reagent |
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Definition
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Term
True or False:
Edwardsiella tarda is a common cause of human diarrhea |
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Definition
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