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Ecotoxicology exam biol3203
Cards with the six answers and some references
85
Environmental Studies
Undergraduate 4
04/27/2014

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Cards

Term
Physiological responses to pollutants
Definition

Respiration

Cardiovascular

Oxidative metabolism (swimming)

Osmotic and ionic regulation

Feeding & Nutrition

Reproduction

Neurological

Behavioural.

Term
Respiration
Definition

Can be Measured by O2 consumption or CO2 production.

Respiratory stress caused by Cu is worse in Cypridae than Onchorhyncus mykiss. It is not related to tissue damage but to disruption in ionoregulation. (DeBoeck 2006)

Term
Cardiovascular
Definition

Heart rate BPM.

Cu increases HR in crabs (Brown 2004)

But this does not measure the stroke volume so circulation may not increase, could be as a clearance measure (Spicer 2007)

Term

Oxidative metabolism and swimming

 

Definition

Ucrit is a measure of swimming speed.

Environmental ammonia reduced the swimming speed of Onchorhyncus mykiss (Wicks 2002)

Term
Osmotic & Ionic regulation
Definition

Ion uptake rate.

Low pH and Al reduce the sodium uptake in pike fry. (Keinanen 2000) Could be because of denaturing channel and ion exchange proteins.

Term
Feeding and Nutrition
Definition

Vitamin profiles, feeding rates, foraging success. Can be affected by a broad range of contaminants. Ammonia reduces feeding in Onchorhyncus mykiss(Wicks & Randal 2002)

Silver nanoparticles effect gut microbiota (Merrifield 2013)

PCB effect vit A&E profiles in Delphinapterus leucas (DeForges 2013)

Term
Reproduction
Definition

Survival of offspring, No. produced, condition of organs.

Correlation between PCB/DDT concentration and the occurrence of lesions on ovaries in Delphinapterus leucas (Beland 1993).

Oestrogenic compounds can cause intersex in fish.

TBT imposex in Nucella lapulis

Term
Neurological and Behaviour
Definition

Lipid soluble compounds can enter neurones via the myelin sheath and may be able to cross the blood brain barrier.

Mercury causes neurone degeneration which can affect behaviours and may be linked to mass strandings of cetacea. Prevents GTP from binding to tubulin. Blocks calcium channels preventing synaptic transfer of action potential. 

Term
Environmental protection
Definition

Toxicity equivalents. Limits of tolerance. Development of biomarkers. Risk assessment. Pronlem formulation, risk analysis and characterisation.

Where do end points fit in. Which approaches and why.

Term
Dose response relationship
Definition

Toxicity is relative: dose and properties determine whether a negative effect is observed.

Environment has a role to play, i.e. availability.

Dose is the amount of substance per body weight or liquid concentration.

Term
Dose response parameter
Definition
Can be for one dose at differnt times or set time with different concentrations.
Term
Toxicity
Definition
Exposure time and dose
Term
Molecular basis
Definition

Toxins effect specific receptor sites.

Dose response is maximal when all receptors are bound to or no more disruption can occur.

Changes in the conformation of enzymes.

Effexts in the nervous system.

 

Term
Dose response assumptions
Definition

Response proportional to concentration at target site.

Concentration at target site is related to dose.

The response is causally related to the compound investigated.

DISCUSS THESE HARD!?

Term
End points of dose response
Definition

LOAEL
NOAEL
LC50

LD50

Term
Pharmacological use of dose response
Definition

Therapeutic index = LD50/ED50 

Seperate the lethal and positive effects.

Term
LC50
Definition

Lethal concentratio responsible for 50% of the deaths.

Is responsible for most of the deaths but some organisms will be sensitive/resistant.

Term
Response measured.
Definition

Question dependent/

Quantal: death, presence/absence of lesions, behaviours.

Graded: Biochemical change, change in growth rate.

Term
Threshold effects.
Definition

Threshold dose: no effect is measurable,

Concentration at which the response is not observed. NOAEL

Not observed in carcinogens.

Limited by curve fitting ability and graph making. Number of data points has an effect.

Term
Safety factors
Definition

NOAEL x 10, 100, 1,000 etc.

e.g Acceptable daily intake = NOAEL mg kg-1d-1/100

WQS = NOAEL/1,000

Term
Limitations
Definition

NOAEL not always present.

Data analysis- curve fitting, number of data.

With NOAEL: decide on acceptable level above background.

NOAEL doesn't mean safe and long term exposure is much harder to test for. 

Often ethical and practical considerations prevent investigation of higher organisms.

Term
Benefits
Definition

Universally accepted techniques give replicable results.

Gives information about which chemicals are most damaging to organisms inn general.

Term
Biomarkers
Definition
Biological response to chemicals or radiation that gives a measure of exposure at cellular, tissue, organ, individual or population level (Peakall 1994).
Term
Biomonitoring
Definition
Frequent or continula gathering of information from a given population that is relevant to that populations health risk.
Term
Types of Biomarker
Definition
histological, biochemical, physiological, genetic, behavioural.
Term
Chronic multiple exposure
Definition
Long term exposure to multiple contaminants at low levels mean that it is difficult to attribute changes to specific contaminants
Term
Natural cycles
Definition
Natural cycles within organisms may be responsible for any number of physiological changes. E.g Crassostrea gigas haemocytes are governed by the seasons as well as gametogenesis, and other environmental stressors. (Duchemin 2007)
Term
Multiparametric approach
Definition
Using information from a multitude of biomarkers (give examples) some researchers have attempted to create mathematical models for prediction of pollution gradients. These have mixed success with the largest obstacle being environmental factors and determination of reference sites,(Auffret 2006)
Term
Background research
Definition

need to have an understanding of how the organism works and what chemicals do to it.

This can lead to the use of inappropriate sentinel organisms. For example mytilus are well studied and used in ecotoxicological experiments due to their economic importance. However there are molluscs that have increased sensitivity to pollutants that would be more suitable but are not used e.g Cerrastoderma edule (Giron-Perez 2009)

Term
Routes of entry
Definition
Need to be fully understood. No use using an organism as a biomarker for a pollutant if it is completely insensitive or impermeable to that contaminant.
Term
Standardised protocols
Definition
Expensive and lengthy investigations to develop best protocols. Need to be agreed upon. Differences between sexes need to be accounted for.
Term
Cryptic stage
Definition
Use the most sensitive stage of the organism to indicate what the worst impast are
Term
Trophic level in biomarkers
Definition

HIgher levels will show more damage from persistent bioaccumulated chemicals. Lower are more easily used- ethical issues, abundance, husbandry.

Some organisms employ different feeding strategies within species. For example orca can be divided into marine mammal feeders and piscivores. If antioxidant activity in blubber samples was used for a biomarker indicating mercury poisoning than the higher trophic level marine mammal eaters would be more likely to display an effect so it would be necessary to distinguish between the two subspecies.

Term
Migrating animals
Definition
Problematic due to range of exposure as they travel through different environments. Can still be used where biological effect is short term such as HSPs but not for something like organ damage.
Term
Normal levels
Definition
Difficult to determine for gene expression and enzyme activity but not for histopathology, though this requires euthanasia of organisms.
Term
Laboratory test species
Definition
Well studied and understood. Genome known, sensitivities known but not suitable for in situ studies.
Term
Biomonitoring VS Chemical assay
Definition
Less specific but more directly linked to ecosystem health. Can detect impacts of combined contaminants. Cannot id contaminants
Term
Sentinels
Definition
Demonstrate presence of bioavailable contaminants and extent of exposure
Term
Surrogates
Definition
Indicate potential human effects
Term
Predictors
Definition
long term effect on ecosystem
Term
Moore et al 2004
Definition
Neutral red dye retention assay used in black sea molluscs to determine stability of lysosome. Low times indicate membrane damage but not which chemical caused it.
Term
Routes of Entry Intro
Definition
Across body surface, through GEO, through GIT
Term
Integument of aquatic organisms
Definition
Fish: epidermis, dermis (scales), hypodermis.
Crustacea: cuticle, exoskeleton, moult cycle.
Soft bodied inverts: Epidermal layer.
Term
Integument compounds
Definition
LMW, lipophilic
Term
Fish skin
Definition
Thinner and more permeable then mammals. Maybe be very thin layer to the systemic circulation. Mucosal layer presents no barrier to diffusion of ions (Shephard 1981)
Term
Skin consequences
Definition
Depends on: frequency of exposure & effects on blood flow and distribution
Term
Plasma concentration
Definition
influence blood:tissue equilibrium
Term
Small molecules
Definition
Utilise pores in capillaries. May Dissolve in the lipid membrane
Term
Hg2+ Mercury
Definition
Can diffuse through calcium or sodium ion channels. May also diffuse across the cell membrane at physiological pH
Term
Metal poisoning
Definition
Induction of metal chaperones and & oxidative stress. Cu is particularly bad.
Term
Through the skin
Definition
local damage of muscles-point of entry. Depends upon point or diffuse source from the environment.
Term
Gastro intestinal tract (GIT). GUT
Definition
Broadest range of contaminant exposure and absorption.
Term
Food
Definition
Contaminants that bioaccumulate on or within food items.
Term
Feeding
Definition
Ingest contaminants in sediment and water that is eaten.
Term
Gut microbiota
Definition
Bioactivation of compounds by bacteria. Make compounds bioavailable and/or more toxic. Also negatively impacted by contaminants e.g. silver nanoparticles (Merrifield et al 2013), which alter the community composition as they are toxic to some,potentially beneficial, of the gut microbiome.
Term
Vetenary products
Definition
In the runoff from land (faecal and aquaculture waste).
Term
Differences in species
Definition
Anatomical and feeding behavioural. Tilapia stir up sediment while feeding and zebra fish feed in the water column, differences in Hg concentration in the fish tissue.
Term
Water chemistry differences
Definition
As salinity increases Cd uptake in Fundulus heteroclitus decreases as determined by radioisotope measurements of concentration in tissue. Also found that Cd decreases in gills and head but increases in the viscera indicating that increase in drinking for osmoregulation is maintaing some Cd (Dutton & Fisher 2011).
Term
Absorption
Definition

Lipid soluble-Throughout GIT.

Weak acids-Stomach.

Weak bases and acids-Small intestine.

Strong asids/alkali-Carrier mediated throughout.

Phagocytosi e.g botulinum

Term
pH
Definition

Affects uptake. Oesophagus-Neutral

Stomach-pH 2

Anterior intestine-pH 6

Term
GIT Consequences
Definition

Lipophilic compounds in fatty tissues.

Metals to the gills, liver and kidney.

Mostly non polar molecules affect the brain.

Expression of chaperones.

Biliary excretion.

Term
Biliary excretion
Definition

Contaminants sequestered in bile then reabsorbed via the biliary duct and hepatic portal vein.

Toxic metabolites returned to the gut.

Increases hepatic exposure.

Saturated bile can cause liver necrosis.

Increases the half life of contaminants.

Term
Route of entry: Gill
Definition
G.E.O. with large SA/V ratio. Short diffusion distance and excellent blood supply, only a few layers of cell between environment and circulatory system.
Term
Mucous cells
Definition
Offer some protection but reduce efficiency of the gills exchange processes.
Term
Contaminants cross the gill
Definition
Lipophilics, metals and small molecular weight organics.
Term
Consequences of gill.
Definition

Damage to gill secondary lamellae e.g. hyperplasia caused by irritation.

Rapid distribution thoughout body.

Reduced excretion and gas exchange capability.

 

Term
Distribution through circulation
Definition

Some contaminants such as copper can bind to plasma proteins, in Cu case albumin.

These restrict distribution to organs but enhance uptake to blood by lowering the plasma concentration.

Saturation of the blood may be the toxic threshold.

Term
Endocrine system
Definition
System of glands producing hormones that are distributed throughout the body to taget systems using the circulatory system for transport. Glands: Hypothalamus: Pituitary: Pineal: Adrenal: Gonadal: Thyroid: Parathyroid: Pancreas: GIT.
Term
Endocrine disruption
Definition
Occurs when an exogenous substance intereacts with any element of the endocrine system causing change in function.
Term
Hormones
Definition
Peptides, Steroids or Amines.
Term
Steroids
Definition
Made from lipids such as cholesterol, most sensitive to anthropogenic chemicals. Produced mainly in the gonads.
Term
Main steroids
Definition
Testosterone, Estradiol and Glucocorticoids.
Term
Steroid modes of action
Definition

Genomic: Steroid binds to receptor and translocates across cell membrane then diffuses into nuclear membrane and directly effects gene expression.

Non genomic: Steroid binds to receptor on cell surface and initiates autophosphorylation such as cAMP, producing an effect such as membrane bound ion channel modulation (Steinman & Trainor 2007).

Term
Target cell disruption
Definition
Blockers and mimics. Blockers prevent steroid binding. Mimics bind and cause an insufficient or excessive response that is generally inappropriately timed.
Term
Hormone sensitization
Definition

Jansen 2004

Increase expression of hormone receptors by inhibitting the action of histone deacetylase which would otherwise reduce expression.

 

Term
Biomarkers
Definition
Endocrine disrupting effects have been well studied in some species. For example tributyltin TBT causes imposex in the dog whelk (Nucella lapulis) (Smith 2006). In females a penis developed to block the oviduct eventually leading to sterility.
Term
Mallard duck shells
Definition
Exposure to DDT causes a reduction in shell mass. This is likely caused by the 65% in Ca-ATPase activity. (Kolaja 1977)
Term
Thyroid hormones.
Definition
Critical for growth and development particularly brain and nerone development.
Term
Thyroid disruption in Orca
Definition
Villanger et al 2011 recorded that the blubber concentration of PCB PBDE and HCB were inversely correlated to thyroid hormone levels.
Term

Alligator example

DDT DDE DDD

Definition

Metabolite of DDT apear to have caused changes in the levels of sex hormones in alligators in lake apopka.

 

Term
Alligator effects
Definition
Reproductive issues. Hormone imbalance. Low hatching rates, physical abnormalities e.g. multiple eggs in one folicle. Genitals not producing properly. Increased estrogen to testosterone ratios in both sexes. ( DDT metabolites interacting with sex organs during development (Harrison 1999).
Term
Crassostrea gigas fisheries in france.
Definition
TBT an antifoulant caused massive reductions in spatfall. This suggests that TBT was acting on the settling mechanism. Shell deformities in adults could be due to calcium metabolism being altered. (Rulz 1999)
Term
Intersex in roach
Definition
Endogenous estrogen immitation by exogenous compound. Males with developing eggs. Vitellogenin synthesis initiated in liver. This is precursor to egg yolk. Caused by estrogen contamination from sewage (contraceptives)(Larsson 1999).
Term
Dioxins and vitellogenin induction
Definition
Dioxins stop vitellogenin production. They cause AHR to form a complex with a nuclear translator and disrupt the estrogen receptor α (ERα) autoregulatory transcriptional loop (Bemanian 2004).
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