Term
Replication Pattern of DNA |
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Definition
This occurs in (S) Synthesis Stage of Interphase |
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Is the method of DNA replication; One old strand (Parent), and one new (daughter) strand combine |
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Hydrogen bonds are being broken during this method |
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replicated original, built toward the fork. |
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Primase and DNA Polymerase III
4 Stages: (1 & 2) |
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Definition
1)Primase-builds RNA, being built in a 5 prime to 3 primse, binds to leading strand
2)DNA Polymerase III-can make DNA if it has a 3 prime strand to bind at; will finish rest of strand towards fork; will build one continuous strand until fork |
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Term
Primase and DNA Polymerase III
4 Stages: (3 & 4)
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Definition
3) DNA Polymerase I-removes RNA primer from leading strand
4)DNA Ligase |
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Term
Lagging Strand: Part I
4 Processes (1 & 2) |
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Definition
1) Primase-makes primer; RNA primer lays multiple primers on legging strand
2) DNA polymerase III-builds sections of DNA between primer RNA; Okazaki fragments |
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Are the sections of DNA that DNA polymerase III builds between primer RNA. |
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Term
Lagging Strand: Part I
4 Processes (3 & 4): |
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Definition
3) DNA polymerase I-
1-will remove ALL RNA primers
2- Replaces those missing areas with DNA
*DNA polymerase CANNOT remove RNA here, so daughter legging strand will be shorter than parent strand, CANNOT attach to Okazaki fragments.*
4) DNA Ligase- seal gaps between DNA built by Polymerase III and DNA built by polymerase I. |
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CANNOT attach to 5 prime end, making daughter strand shorter |
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protection of genetic code; our aging line.
IE: Longer telemere=you appear younger looking
Shorter Telemere= you appear older; are aging |
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Produces telemerase; diploid cells that are destined to become egg or sperm; have an enzyme called telemerase |
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replaces telemeres after each replication |
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is a spot on the DNA molecule; where a replication fork is programmed to occur.
*repilcation bubble* |
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Begins with helicase begins the *BUBBLE* |
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reduces tension on the DNA molecule portions that are upstream and downstream from the bubble.
*Is and SSBP*
SSBP-Single Stranded Binding Protein |
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3'(Prime) ---->5'(Prime), fork;
5' ends up being at the FORK |
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5'---->3', AWAY from fork |
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lay down an RNA primer that is anti-parallel to the parent strand |
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binds the daughter(new) DNA strands, BUT can only add nucleotides to a 3' end |
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removal and replacement of RNA primer with DNA |
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seals gaps in daughter strands(new) |
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2 Names of parent strands: |
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1) Leading Strand
2)Lagging Strand |
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SSBP(Sing Stranded Binding Protein)- |
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maintain separation of the parent strands and stabilize each strand; single strand because it only binds to ONE strand! |
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an area of DNA molecules that is being replicated between 2 forks |
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Definition
Unwinds the DNA parent strands within the bubble; BEGINS Replication Bubble |
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