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DNA Technology
AQA Biol 5
13
Biology
12th Grade
05/12/2015

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Cards

Term
What is the function of restriction endonuclease?
Definition
Recognition and cutting of DNA at specific base sequences
Term
What is the function of DNA polymerase?
Definition
Formation of a new DNA strand during replication
Term
What is the function of reverse transcriptase?
Definition
production of a cDNA fragment from an mRNA strand
Term
What is the function of DNA ligase?
Definition
Binding of DNA fragments
Term
What is needed for PCR?
Definition
-Primer
-Template DNA
-DNA nucleotides
-DNA polymerase
Term
What is the function of the DNA fragment?
Definition
subject material to be copied and sequenced
Term
What is the function of the primer?
Definition
Allows the DNA replication process to start
Term
What is the function of ddNTP?
Definition
Terminates DNA replication at each instance of a specific base
Term
What is the function of nucleotides?
Definition
Raw materials for DNA replication
Term
What are liposomes?
What can they be used for in gene therapy?
Definition
Spheres of lipid that can fuse with the cell membrane to release their content into the cell
They can carry plasmids into the cell
Term
What does germ line gene therapy involve modifying?
Definition
Gametes
Term
What does somatic cell gene therapy involve modifying?
Definition
Body cells
Term
What is gene therapy?
Definition
When defective genes are replaced or supplemented by a functioning one
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