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DNA structure and Analysis
Genetics
24
Science
Undergraduate 4
03/19/2009

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Cards

Term
Levine
Definition
4 similar nucleotides exist in equal amounts and repeated
Term
Friedrick Miescher
Definition
isolation of acid substance containing DNA from nuclei
Term
Erwin Chargaff
Definition
showed four nucleotides dont exist in equal proportions
Term
Griffith transformation
Definition
R strain- mouse die
S strain-mouse lives
S strain heat& R- mouse lives
Term
Avery, Macleod, McCarty
Definition
DNA is responsible transforming principle
Term
Hershey-chase experiment
Definition
S35 stayed outside the bacteria and P32 infected into the bacterial cells and directed phage synthesis so that newly arising phages are directed by information on P32
Term
Indirect Evidence
Definition
DNA distribution, Mutagenesis,
Term
DNA distribution
Definition
genetic material must be in the place where genetic functions are performed
Term
Mutagenesis
Definition
wavelengths of uv show efeectiveness of each wavelength in mutagenesis. Nucleic acid absorption spectrum coincides with the most effective mutagenesis wavelenth.
Term
Direct Evidence
Definition
Recombinant DNA technology, Rat encoding growth hormone
Term
What are some evidence that RNA is genetic material in viruses
Definition
Purified RNA from TMV spread on tobaco leaves---> viral lesions appeared on leaves
2) RNA from phage QB replicated by RNA replicase from E.coli cell
3)Retrovirus contains RNA serves as template for synthesis of cDNA by rev transcriptase. ex HIV, RNA tumor virus
Term
Watson and Crick
Definition
DNa double helix, base comp analy of hyrolyz samp of DNA, x-ray diffraction of DNA
Term
Nucleic acid chemistry
Definition
nitrogenous base- pyrimidine CTU, purine (A,G) 2) pentose sugar, 3)phosphate group
Term
difference between DNA and RNA
Definition
presence or absence of O at C-2 postion
Term
Nucleoside
Definition
nitrogenous base + pentose
Term
nucleotide
Definition
nitrogenous base + pentose + a phosphate
Term
nucleotide derivatives
Definition
ATP, GTP
Term
Linus Paulin
Definition
x-ray diffraction study on protein, alpha helix, concluced dna is a triple helix
Term
Rosalind frankin
Definition
x-ray diffraction on purified DNA
Term
Watson-crick model
Definition
chains coiled around a central axis forming a right-handed helix, 2 chains are anti-parallel(opposite direction), bases are perpend to axis stacked 3.4 A, nitrogenous bases of opposite chians are paired as a result of hydrogen bonds, each complete turn of the helix 3.4A long, alternating major grooves, and minor grooves exist along axis, double helix measures 20A
Term
Watson-Cric DNA form B-form
Definition
under aqueous, low salt condition
Term
watson-crick DNA form
Definition
high-salt or dehydrated condition
Term
watson-crick form z-form
Definition
from synthetic DNA consisted of G and C only left handed double helix in zigzag
Term
Structure of RNA
Definition
base U replaces T, presence of an Oxygen atom
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