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4 similar nucleotides exist in equal amounts and repeated |
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isolation of acid substance containing DNA from nuclei |
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showed four nucleotides dont exist in equal proportions |
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R strain- mouse die S strain-mouse lives S strain heat& R- mouse lives |
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DNA is responsible transforming principle |
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S35 stayed outside the bacteria and P32 infected into the bacterial cells and directed phage synthesis so that newly arising phages are directed by information on P32 |
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DNA distribution, Mutagenesis, |
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genetic material must be in the place where genetic functions are performed |
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wavelengths of uv show efeectiveness of each wavelength in mutagenesis. Nucleic acid absorption spectrum coincides with the most effective mutagenesis wavelenth. |
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Recombinant DNA technology, Rat encoding growth hormone |
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What are some evidence that RNA is genetic material in viruses |
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Purified RNA from TMV spread on tobaco leaves---> viral lesions appeared on leaves 2) RNA from phage QB replicated by RNA replicase from E.coli cell 3)Retrovirus contains RNA serves as template for synthesis of cDNA by rev transcriptase. ex HIV, RNA tumor virus |
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DNa double helix, base comp analy of hyrolyz samp of DNA, x-ray diffraction of DNA |
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nitrogenous base- pyrimidine CTU, purine (A,G) 2) pentose sugar, 3)phosphate group |
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difference between DNA and RNA |
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presence or absence of O at C-2 postion |
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nitrogenous base + pentose |
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nitrogenous base + pentose + a phosphate |
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x-ray diffraction study on protein, alpha helix, concluced dna is a triple helix |
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x-ray diffraction on purified DNA |
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chains coiled around a central axis forming a right-handed helix, 2 chains are anti-parallel(opposite direction), bases are perpend to axis stacked 3.4 A, nitrogenous bases of opposite chians are paired as a result of hydrogen bonds, each complete turn of the helix 3.4A long, alternating major grooves, and minor grooves exist along axis, double helix measures 20A |
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Watson-Cric DNA form B-form |
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under aqueous, low salt condition |
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high-salt or dehydrated condition |
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from synthetic DNA consisted of G and C only left handed double helix in zigzag |
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base U replaces T, presence of an Oxygen atom |
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