Term
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Definition
| DNA to RNA to protein--> discovery of reverse transcriptase |
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Term
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Definition
| As DNA unwinds, viscosity decreases and uv absorption increases, Higher GC contents, higher melt temp, Melting temp of DNA with more GC contents is higher than that of DNA with less GC contents |
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Term
| Molecular Hybridization Techniques |
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Definition
| in situ hybridization: single stranded DNA or RNA is added to the cell FISH- fluorescent in situ hybridization |
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Term
| Reassociation Kinetics and Repetitive DNA |
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Definition
DNA is sheared to average several hundred nucleotides-->heated to denature-->lower temperature--> moniter reassociation of DNA, larger
the genome size the slower the
reassociation size, repetitive
sequences quickly reassociate and unique sequences reassociate slowly |
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Term
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Definition
| nucleic acids can be separated, nucleic acid has phosphate group, and th number of phosphate group is proportional to the lenght of nucleic ancids, pore size of the matrix amid gel for protein, agarose gel can be varied |
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