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Diagnosing Infection
Test Four
73
Microbiology
Undergraduate 3
03/12/2013

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Cards

Term
What do you need to diagnose infectious diseases?
Definition
complete patient history, physical exam, evaluate symptoms, proper selection, collection, transport and processing of clinical specimens
Term
What are clinical specimens?
Definition
body fluids, secretions, tissues
Term
What is important of clinical specimens?
Definition
that they are taken correctly because everything else relies on these collections
Term
What are needle sticks
Definition
blood cultures, and are the biggest danger for occupational hazard
Term
how are clinical specimens protected?
Definition
leakproof container and proper labeling
Term
What does garbage in garbage out mean?
Definition
bad data in? bad data out
Term
What do high quality specimens give you?
Definition
clinically relevant results
Term
What are the three components of specimen quality?
Definition
proper selection, collection and transport
Term
what are the three consequences of poor-quality specimens?
Definition
1) etiologiv agent may be missed/destroyed
2) overgrowth of indigenous flora may mask etiologic agent
3) contaminants may interfere with recovery of etiologic agent
Term
What does the lab provide to lay out laws of specimen collection
Definition
written guidelines
Term
what is the floor manual?
Definition
what the lab is responsible for, it is required for certification and tells you how to handle each thing that could happen
Term
How are specimens properly collected?
Definition
physician-specified collections and work with the MCB lab
Term
why is proper collection important?
Definition
it minimizes contamination and protects sterile specimens
Term
what is a good way to prevent contamination
Definition
collect speciment from site where pathogen is most likely to be found with the least contamination
Term
When undergoing antimicrobial therapy, when do you obtain the specimen?
Definition
prior to the therapy
Term
When is the best stage to collect specimen?
Definition
the acute stage (when showing symptoms)
Term
What are 4 points about collecting that would make it better?
Definition
perform collection with care and tact, provide instructions for self-collections, obtain a sufficient quantity, and place in a sterile container.
Term
What do you have to protect the specimen from during transportation?
Definition
heat and cold
Term
How are hazardous specimen handled?
Definition
with more care to avoid contamination of personnel
Term
How do containers need to be?
Definition
sterile
Term
What does a proper label include?
Definition
name, ID, culture site, date/time
Term
What does an appropriate request slip include?
Definition
requesting physician, collector, working diagnosis
Term
When should the collected specimens be brought to the MCB lab?
Definition
ASAP
Term
TF urine usually is contaminated by microbes
Definition
F
Term
How do you get a clean sample of urine?
Definition
"clean catch, midstream urine"
Term
Cateterized urine
Definition
use a straight cath - tap bladder and drain. don't take a sample from a full catheter bag
Term
Suprapubic needle aspiration urine
Definition
taps urine through pelvic bone
Term
How does urine need to be treated in the context of lab stuff
Definition
processed within 30 minutes, and refrigerate at 4C for up to 24 hours
Term
In urine samples, is bacteruria alone significant?
Definition
No
Term
How do you tell if the bacteria in urine is due to contamination or not?
Definition
you do a cell count and look for white blood cells in the urine
Term
bacteriaurea
Definition
the presence of bacteria in urine not due to the contamination of the sample.
Term
What do WBC in the urine indicate?
Definition
you know there is an infection (if there is just bacteria then you know it's just a colony from contamination)
Term
Bacteremia
Definition
live bacteria in the blood that does not reproduce, easily cleared
Term
Septicemia
Definition
live bacteria in blood that is multiplying and being sent to other organ systems, eventually kills patient
Term
What is in a blood culture set?
Definition
1 aerobic vial and 1 anaerobic vial
Term
What is the most sensitive to the volume of blood collected?
Definition
10 mL
Term
How do you disinfect blood?
Definition
70% alcohol, iodiphore, and chloro-
Term
How do you clean injection site?
Definition
concentric swabbing motion outward from injection site
Term
Clinical specimens: cerebrospinal fluid: meningitis
Definition
infection of the membrane that surrounds the brain
Term
Clinical specimens: cerebrospinal fluid: encephalitis
Definition
inflammation of the brain
Term
Clinical specimens: cerebrospinal fluid: meningoencephalitis
Definition
you have an infection and inflammation
Term
TF bacterial etiologies are rapidly fatal
Definition
T
Term
Clinical specimens: cerebrospinal fluid: how is it collected
Definition
lumbar puncture
Term
Clinical specimens: cerebrospinal fluid: how fast does the processing need to be?
Definition
30 minutes or less (STAT)
Term
Clinical specimens: cerebrospinal fluid: how are they read in the MCB lab
Definition
gram stain and culture
Term
Clinical specimens: cerebrospinal fluid: how is the glucose count affected?
Definition
it goes down
Term
Clinical specimens: cerebrospinal fluid: how is the preliminary report handled?
Definition
it must be called into the lab, no fax
Term
TF many people die from viral meningitis
Definition
F
Term
Clinical specimens: cerebrospinal fluid: what kind of test is bacterial antigen testing?
Definition
latex test
Term
Sputum
Definition
accumulation of pus within the lungs (where the bugs will be if there is pheumonia)
Term
TF sputum travels well
Definition
T
Term
What is examined for quality control of sputum specimens?
Definition
white blood cells, epithelial cells
Term
What is a bronchial aspiration?
Definition
drawing fluid from the body
Term
What is the significance of first morning sputum?
Definition
no idea
Term
Does sputum travel well?
Definition
yes, it needs to be refrigerated though
Term
What bug do throat swabs usually test for?
Definition
Group A strep; steptococcus pyogenes
Term
What do you have to do for testing throat swabs for anything besides type A strep
Definition
make a special request
Term
What do you have to do with a wound?
Definition
aspirates (removal of fluid) or biopsy
Term
What do you have to do with wounds? 4 things
Definition
swab the specimen, indicate the type of wound, note the anatomic site, and see if it is aerobic or anaerobic
Term
What does a GC culture test?
Definition
neisseria gonorrhoeae
Term
When should GC inoculation occur?
Definition
immediately to the appropriate selective media
Term
What kinds of swabs do you have with GC cultures?
Definition
vaginal, cervical, urethral, throat, rectal
Term
Should GC swabs be refrigerated during transport?
Definition
No
Term
What kind of report do you get for males from GC cultures?
Definition
preliminary gram stain report
Term
What kind of tests are used for GC and chlamydia trachomatis?
Definition
nucleic acid-based tests
Term
What can a binary test test for?
Definition
gonorrhoea and chlymydia
Term
If fecal specimens were not processed ASAP, what would happen?
Definition
die off of bacterial pathogens
Term
What is cultured from a stool sample?
Definition
salmonella, shigella, campylobacter
Term
What parasites are examined from stool specimens?
Definition
protozoa and helminths
Term
If you want to look for other organisms in stool specimens, what do you need to do?
Definition
make a special request
Term
pathology
Definition
the study of the structural and functional manifestations of disease
Term
Who is the doctor's doctor?
Definition
the pathologist
Term
What two types of pathology are there?
Definition
anatomical and clinical
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