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Developmental biology
Developmental Biology quiz 2 lecture 9
21
Biology
Undergraduate 3
07/12/2012

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Term
syncytial blastoderm
Definition
9th division
13 synchronous cleavage cycles
•Nuclei divide within a common cytoplasm
•Nuclei reach the cortex at the tenth division
•At this point each nucleus has their own Energid
•Mitotic apparatus + Nucleus = Energid
cellular bastoderm follows-
Cellular Blastoderm can be inhibited by nocodazole and colchicine
Term
ovum
Definition
-takes 8 days and 14 stages to develop to a mature ovum
-mRNAS are transcribed in nurse cells and transported by MTs into the oocyte
Term
drosophilia fertilization
Definition
only one sperm can enter the micropyle
Term
micropyle
Definition
prevents polyspermy
no orticle granules present
axis formation already happens by the time the sperm enters the egg
Term
Superficial Meroblastic
Definition
– Large amount of yolk
– Cells do not form until after several rounds of karyokinesis (nuclear division)
– Karyokinesis without cytokinesis
– Cell cycle is biphasic
– Cleavage forms a syncytium—256 nuclei with synchronous mitoses
– During the ninth divison—five nuclei at the posterior end become pole cells (gametes)
– Tenth cycle—nuclei migrate to the cortex of embryo and continues cleavage forming a syncythial blastoderm
Term
ration of chromatin to cytoplasm
Definition
DNA keeps increasing in a constant supply of cytoplasm
Term
smaug protein
Definition
RNA binding protein(increase during cleaveage)
-targets maternal RNA for degradtion
-mutations of smaug protein, disrupts normal time of muclear division and prevents celluization
Term
cycle 14
Definition
beginning of cellularization
Term
cell cyle regulators
Definition
required for G-stages in cell cycle
Term
FATE MAP
Definition
aminiserosa==> dorsal ectoderm==>lateral ==>ectoderm==>nuerogenic ectoderm==>mesoderm
Term
Cell Movements in Gastrulation
Definition
•Ventral furrow forms by ingression of cells
expressing Dorsal protein followed by
invagination of mesoderm
•Endoderm invaginates—forms two pockets at
the anterior and posterior ends of the VF
•Germ band extends by convergent extension
•Ingression of other ventral cells will form
neurons and part of nervous system
• Organogenesis, segmentation, imaginal disc
segregation when germ band extended
• Gastrulation movements occur ~20 minutes
Term
Gastrulation
Definition
•Ventral midline invaginates
forming the ventral furrow—
eventually forms ventral
tube
•Ectodermal cells on the
surface and mesoderm—
undergo convergent
extension and forms germ
band and closes-off ventral
furrow
•Germ band forms the
trunk
•Pole cells at dorsalposteior end
•Segmentation begins
•Head and Mouth
•Thorax
•Abdomen
Term
Forward Genetics
Definition
Technique that Determines
the Function of a Particular Gene

caused by x-ray to break DNA or by chemicals (EMS) which causes point mutations
Term
sepcifications of A-P axis
Definition
during oogenesis and before fertilization
Term
gurken
Definition
secreted protein for axis specification
Term
torpedo
Definition
gurken specific receptor
the binding causes the differentiation of terminal follicle cells to posterior follicle cells (posteriorization) -moves follicle cells to the posterior position
Term
PFC(posterior follicle cells)
Definition
intiate protein kinase A which leads to MT's polymerization between oocyte and nurse cells
Term
bicoid
Definition
binds to dyesin(+) end and translocated to anterior of egg

graded anterior morphogen
– represses caudal; up regulates
hunchback
– mRNA localization depends on
Staufen, Exuperantia, Dynein
– mutants lack head and thorax
Term
oscar
Definition
binds to kinesin and moves to the posterior end to join with nanos
Term
D-V Axis
Definition
nudel+pipe bind. the formed protein becomes Gd==>Snake==>==>easter==>spatzle==>toll(on the nucleus membrane)==>pelle tube==>binds to dorsal cactus==>they serperate and dorsal goes into the nuclus of the cell.
Term
NANOS
Definition
posterior morphogen
– represses hunchback
– mRNA localization depends on
Oskar, Staufen, Kinesin I
– mutants lack abdomen
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