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Dental Bacteriology Exam 1
Cultivation
24
Biology
Graduate
08/18/2009

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Term
How do bacteria grow/divide?
Definition
binary fission
Term
What is the Growth Curve?
Definition
depiction of population changes in a bacteria culture
Term
Phases of a Growth Curve:
Definition

1. lag phase - physiological adjustment

2. logarithmic phase - exponential growth with decrease in cell size

3. stationary phase - number of newly produced bacteria equals the number that die

4. decline phase - # dying > # produced

Term
Generation Time:
Definition
average time needed to double the bacterial population. (it is a rough guesstimation of the clinical course of a disease) - determined during logarithmic phase
Term
Hydrogen donors:
Definition
Growth factors that serve as energy sources- are oxidized
Term
Hydrogen acceptors:
Definition
growth factors that are reduced and are used during respiration and fermentation.
Term
How do most bacteria obtain energy
Definition
oxidation of chemical compounds
Term
True or False: All bacteria require a carbon source.
Definition
TRUE
Term
What is the sole source of carbon for the autotroph?
Definition
Carbon Dioxide
Term
How do heterotrophs get carbon?
Definition
carbon dioxide plus an organic form.
Term
Bacteria need a nitrogen source for:
Definition
amino acids, purines, pyrimidines etc.
Term
True or False: Not all bacteria require inorganic salts for growth.
Definition
FALSE- examples are nitrogen, sulfur, phosphorus, potassium, magnesium, iron, zinc and copper.
Term
Growth conditions of Obligate Anaerobes:
Definition
grow only under conditions of high reducing intensity. lack enzymes like catalase to process toxic oxygen products like hydrogen peroxide and O2-. Metabolism involves fermentation.
Term
Catalase:
Definition
breaks down "toxic" byproducts of aerobic respiration like hydrogen peroxide and O2-. Prevents aerobes from killing themselves.
Term
Growth conditions for Aerotolerant Anaerobe:
Definition
will grow in presence of O2 but does not use an electron acceptor.
Term
Growth conditions for facultative anaerobe:
Definition
Capable of growing under both aerobic and anaerobic conditions. will employ aerobic respiration when O2 present.
Term
Growth conditions for Obligate Aerobe:
Definition
Requires oxygen for growth. Metabolism is aerobic respiration.
Term
Growth conditions for microaerophile:
Definition
grows best at low O2 tensions. probably possesses enzymes that are inactivated under strongly oxidizing conditions.
Term
Growth requirements for psychrophiles:
Definition
optimum growing temperature is -5 to 5 degrees celsius
Term
Growth requirements for mesophiles:
Definition
Grow best in temperatures of 30-40 degrees celsius. these are found in humans.
Term
Growth requirements for thermophiles:
Definition
grow best at temperatures of 65-75 degrees celsius.
Term
Optimum pH for most pathogenic bacteria
Definition
pH = 7.2-7.6
Term
Nutrient transport happens by which mechanisms:
Definition
passive diffusion, facilitated diffusion, and active transport.
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