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Dental Bacteriology Exam 1
Gene Organization and Expression
15
Biology
Graduate
08/25/2009

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Term
Regulation of gene expression is determined by:
Definition
the frequency of initiating transcription
Term
A segment of DNA encoding polypeptide is:
Definition
A Cistron
Term
A a functioning unit of key nucleotide sequences of DNA including an operator, a common promotor, and one or more structural genes, which is controlled as a unit to produce mRNA, in the process of transcription by an RNA polymerase:
Definition
operon
Term
the segment of DNA that contains all the information for production of single polypeptide
Definition
Cistron
Term
True or false: Most bacterial genes are monocistronic
Definition
False: most bacterial genes are multicistronic
Term
What is bacterial recombination?
Definition

exchange of genetic information between bacteria. progeny possess a combination of genes which are different (hybrid genomes) from the parental generation.

  • Can be accomplished through 3 mechangisms: transformation, transduction, and conjugation
Term
Naked DNA derived from one cell or virus is taken up by another cell and recombines with the genome:
Definition
Transformation
Term
DNA of one cell is introduced into another cell by virus infection:
Definition
transduction
Term
Generalized transduction:
Definition
viral infection of a donor bacterium causes the denaturation and fragmentation of bacterial DNA. Viral replication and maturation may result in the incorporation of any portion of the bacterial genome into a viral capsid. The viral capsid containing bacterial DNA can infect and introduce the DNA into a recipient bacterium. This DNA may be incorporated into the nucleoid and expressed.
Term
Conjugation:
Definition

*bacterial sex.

*Males are F+ and Females and F-

*gene F promotes the formation of a sex pilus and replication of itself

Term
Extrachromasomal genetic elements capable of autonomous replication
Definition
plasmid
Term
segments of double stranded DNA that can move from place to place within a genome or between genomes.
Definition
Transposon
Term
True or False: transposons carry genes controlling their own replication.
Definition
False: they do NOT carry genes controlling their own replication, but possess insertion sequence elements which are responsible for transposability.
Term
Small sequences of DNA that code for site specific recombination.
Definition
insertion sequences
Term
Cloning vectors:
Definition
Carriers of foreign DNA that are used to introduce recombinant DNA in suitable hosts for replication and/or expression. The replicons or products derived from them are used in diagnosis adn therapy of microbial diseases.
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