Term
| Numerical Aperture Formula |
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Definition
NA = nsinθ
remember to half the aperature angle
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Term
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Definition
| The minimum distance or angular separation between two point objects which allows them to be resolved |
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Term
| Limit of Resolution Formula |
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Definition
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Term
| Two things affecting limit of resultion |
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Definition
| Wavelength and numerical aperature. |
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Term
| What emits electrons into the vacuum in an electron microscope? |
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Definition
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Term
| How does the e-gun emit electrons into the vacuum? |
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Definition
| The current heats the filament and electrons boil into the vacuum. |
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Term
| What is the purpose of placing tissue in 30% sucrose? |
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Definition
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Term
| Refraction definition and cause |
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Definition
Light bending as it changes speed when it passes through the interface of two mediums.
Ex. glass/air or glass/oil |
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Term
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Definition
| Where rays that propagate in two perpendicular planes have different foci. The vertical and horizontal lines are in sharp focus at two different distances. |
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Term
| Constructive Interference |
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Definition
When the crest of one wave passes through, or is superpositioned upon, the crest of another wave, we say that the waves constructively interfere. Constructive interference also occurs when the trough of one wave is superpositioned upon the trough of another wave. Amplitudes increase.
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Term
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Definition
When the crest of one wave passes through, or is superpositioned upon, the trough of another wave, we say that the waves destructively interfere. Amplitudes subtract.
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Term
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Definition
| A transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. |
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Term
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Definition
| Fixation preserves a sample of biological material (tissue or cells) as close to its natural state as possible in the process of preparing tissue for examination. |
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Term
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Definition
| Oxidation of macromolecules such as lipids leads to atoms becoming cross-linked. It is also used by researchers to stain biological things in order to image them using transmission electron microscopy, by embedding a heavy metal directly into the cell. |
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Term
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Definition
| Glutaraldehyde is used in biological electron microscopy as a fixative. It kills cells quickly by crosslinking their proteins and is usually employed alone or mixed with formaldehyde[3] as the first of two fixative processes to stabilize specimens such as bacteria, plant material, and human cells. A second fixative procedure uses osmium tetroxide to crosslink and stabilize cell and organelle membrane lipids. |
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Term
| Why you dont use Glutaraldehyde with fluorescent: |
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Definition
| Glutaraldehyde can produce fluroscent properties and fuck your shit up. |
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Term
| Which microscope requires a secondary fixative? |
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Definition
| Electron microscope. Can be osmium tetroxide. |
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