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Most sensitive stain for granulocytes Differentiates AML from ALL Lymphoblasts do not stain AML+ ALL- |
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Differentiates AML from ALL Lymphoblasts do not stain Stains primary and secondary granules in granulocytes Also stains Auer Rods -Found in myeloblasts and granulocytes AML+ ALL- |
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Chloroacetate Esterase Specific Stain |
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Definition
Differentiates AML from ALL Negative for Acute Eosinophilic Leukemia (AEL) AML+ ALL- |
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Definition
Diagnoses Acute Myelomonocytic Leukemia and Acute Monocytic Leukemia Monoblasts stain intense Also stains plasma cells, hairy cells, T-lymphocytes AML+ ALL- |
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Definition
Diagnoses ALL, Acute Erythroid Leukemia, and Megakaryoblastic Leukemia Normal erythroblasts do not stain with this AML- ALL is negative or “chunky positive) Identifies abnormal glycogen metabolism |
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Definition
Distinguishes Leukemoid Reactions/Reactive Neutrophilia from CML CML- Found in Leukemoid Reactions |
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Definition
Hairy Cell Leukemia is positive Mast cells are positive |
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Definition
Positive in Thymus Lymphocytes and Pre-B Lymphocytes -Found in ALL Distinguishes ALL from Malignant Lymphoma DNA polymerase Primitive Cell Marker |
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Definition
Positive for mast cells and basophil cells Negative for hairy cells |
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Reticulin Stain and Masson’s Trichrome Stain |
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Definition
Identifies the presence and extent of fibrosis -Will see fibrosis in Myelofibrosis |
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