Term
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Definition
Enzymes that cut up foreign DNA.
Enzymes that can cut the host's DNA but only on restriction sites. (recognition sequences) |
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Term
| Restriction Sites (Recogntion sites) |
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Definition
| Base sequences on the host's DNA that are the site where restriction enzymes cut. |
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Term
| How does the host protects its DNA from its restriction enzymes? |
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Definition
| (page 323) The cell modifies its restriction sites by adding methyl groups (-CH3). The purpose of methyl groups to make the the restriction sites unrecognizable to the restriction enzymes. |
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Term
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Definition
(page 324)
It is two base sequences that can be read from 5' end and it is the same base sequences.
Example:
5'...GAATTC...3'
3'...CTTAAG...5'
In English class, an example of palindrome is mom, dad, level, otto, and much more. |
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Term
| Purposes of Restriction Enzyme Digestion |
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Definition
(page 324)
- To identify and analyzed point mutations
- Cuts up DNA for PCR amplification
- Cuts up DNA for Gel Electrophoresis
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Term
| What three things can Gel Electrophoresis reveal? |
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Definition
- The number of fragments
- The size of the fragments
- The relative abundance of a grament
(Page 324) |
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Term
What is DNA fingerprinting? |
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Definition
Using restriction digestion and gel electorphoresis to identify an individual based on their DNA fragments.
(see page 325) |
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Term
Sing nucleotide polymorphisms (SNPs) |
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Definition
Inherited variations involving a single nucleotide base. SNPs can be anywhere in the chromosome even in a restriction site. |
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Term
Short tandem repeats (STRs) |
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Definition
short, repetitive DNA sequences that are usually found in the noncoding region of the chromosome.
Example:
AGGAGGAGGAGGAGGAGG |
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