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Crude Protein
Crude Protein & Nitrogenous nutrients
27
Nutrition
Undergraduate 3
09/28/2014

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Cards

Term
What is the definition of crude protein?
Definition
total nitrogen content X 6.25

(average N content in feed is assumed to be 16g N per 100g protein
Term
What are the nitrogenous nutrients in crude protein?
Definition
Proteins
Oligpeptides & short peptides
Free amino acids
DNA/RNA/nucleotides/nucleosides/polyamines
Term
Proteins (3 points)
Definition
-major N-containing group in feed
-made of various AA residues
-free AA and short peptides are released upon enzymatic hydrolysis & absorbed
Term
Oligopeptides & short peptides (2 points)
Definition
-found in smaller quantities in feed
-enzymatic hydrolysis releases free AA & short peptides & absorbed
Term
Free Amino Acids (2 points)
Definition
-rare in conventional feeds
-enzyme/chemical hydrolysis treatments of protein supplements can increase free AA levels as well as oligopeptides & short peptides
Term
DNA/RNA/Nucleotides/Nucleosides/Polyamines
Definition
-small quantities in conventional feed
-high quantities in DDGS, dairy foods, sea foods
-DNA & RNA digested & absorbed as nucleotides and nucleosides
-promote trophic cellular growth
Term
Major biosynthetic roles of AAs

(5)
Definition
1. Substrates for bioactive peptide & protein synthesis (for hypertrophic cellular growth)

2. Signaling molecules to regulate hypertrophic & hyperplasic cellular growth, cellular metabolism

3. Precursors for nucleotide & nucleic acids

4. Precursors for glucose/ketone body synthesis, fatty acids (monogastrics) & glycogen (all animals)

5. Precursors for synthesis of neurotransmitters or acting directly as neurotransmitters
Term
What pathway are AAs involved in which regulates cellular growth & metabolism
Definition
mTOR signalling pathway
Term
What amino acids act as precursors for synthesis of neurotransmitters?
Definition
L-His
L-Tyr
L-Trp
L-Arg
Term
What amino acids can act directly as neurotransmitters?
Definition
Gly
L-Asp
L-Glu
L-Tau
Term
What are the nutritional/metabolic roles of AAs, nucleotides and polyamines?
Definition
AAs:
-metabolic fuels (ATP production)
-buffering solutes to maintain physiological fluid pH
-maintaining osmolarity in physiological fluids

Nucleotides & polyamines:
-essential in trophic organ & tissue growth
Term
What amino acids are essential in neonatal mammals?
Definition
Arginine & Proline
Term
What amino acids are semi-essential in adult mammals?
Definition
Cystein
Tyrosine
Term
What diet is recommended to meet the protein needs of calves?
Definition
milk or milk replacers containing milk protein (casein)
Term
What diet is recommended to meet the protein needs of dry cows
Definition
Poor quality hay/roughage to maintain rumenal microflora, no need for protein supplements
-are not growing and do not need to produce milk protein
Term
What diet is recommended to meet the protein needs of lactating cows?
Definition
Good quality protein supplements
e.g. soybean meal, linseed meal, canola meal, sunflower meal
-need to maintain ruminal microflora & synthesize body and milk protein
Term
What diet is recommended to meet the protein needs of beef steer & replacement heifers?
Definition
Good quality protein supplements
e.g. soybean meal, linseed meal, canola meal, sunflower meal
-need to maintain ruminal microflora & synthesize body protein
Term
What diet is recommended to meet the protein needs of monogastric herbivores?
Definition
grass or poor quality hay if not in work or producing

soybeal meal or other oil seed meal supplements should be given to working/reproducing animals to meet their crude protein & AA requirements
Term
What diet is recommended to meet the protein needs of monogastric avian species?
Definition
High quality protein supplements needed to meet CP & AA requirements
e.g. soybean meal, canola meal, soybean protein concentrate, fish meal, meat & bone meal, hydrolyzed feather meal

-crystalline essential AA supplements to meet the AA requirements, especially if being fed a plant-based protein source
Term
What diet is recommended to meet the protein needs of neonatal pigs?
Definition
-sows milk or milk replacer containing milk protein
-high quality protein supplements e.g. soybean protein isolate, canola meal protein isolate
Term
What diet is recommended to meet the protein needs of weanling swine?
Definition
High quality protein supplements
e.g. soybean meal, soybean protein concentrate, fish meal, hydrolyzed feather meal

Further supplementation with crystalline essential AA's
Term
What diet is recommended to meet the protein needs of reproducing swine, growing-finishing pigs and feeder pigs?
Definition
supplementation of soybean meal, canola meal, sunflower meal
-further supplementation of crystalline AAs
Term
What diet is recommended to meet the protein needs of dogs?
Definition
-plant protein supplements are not very palatable
-prefer poultry meal, beef meal, bone meal, rendered meat meal etc
Term
What diet is recommended to meet the protein needs of cats/minks/foxes?
Definition
-plant sourced proteins are not palatable/digestible
-prefer poultry meal, beef meal, bone meal, rendered meat meal etc
Term
What diet is recommended to meet the protein needs of shrimp/fish?
Definition
-soybean meal, soybean protein isolate, canola protein isolate, fish meal, meat & bone meal
Term
How are crystalline pure essential AA's produced?
Definition
via microbial fermentation
Term
What are the common crystalline pure essential AAs?
Definition
DL-Met
L-Met
L-Thr
L-Trp
DL-MHA
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