Term
| what is the functional domain of the antibody |
|
Definition
|
|
Term
| what is antigen also called |
|
Definition
|
|
Term
| what is the epitope or antigenic determinant |
|
Definition
| the portion of an antigen involved in the reaction with an antibody |
|
|
Term
| what determines antigenicity |
|
Definition
| the tertiary shape of the molecule and the degree of "foreignness" between "self" and the antigen |
|
|
Term
|
Definition
| when a antigen is structurally similar to the homologous antigen to which it was produced |
|
|
Term
| what kind of reactions are easily visible to the unaided eye |
|
Definition
|
|
Term
|
Definition
| when the antibody is in excess |
|
|
Term
|
Definition
| when the antigen is in excess |
|
|
Term
| what is the zone of equivalence |
|
Definition
| generally when there are 2-3 antibody molecules per each antigen |
|
|
Term
| what assay is charachterized by measurable rings of precipitaiton |
|
Definition
|
|
Term
| what is a drawback of radial immunodiffusion |
|
Definition
| the long diffusion period |
|
|
Term
| what is the assay where two wells are cut into a plain gel and the antibody and antigen diffuse toward each other |
|
Definition
Double diffusion technique
ouchterlony technique |
|
|
Term
| what is used to identify monoclonal proteins in serum and urine |
|
Definition
|
|
Term
| when do a fixed amount of labeled antigen competes with unlabeled sample antigen for a limited number of antibody sites |
|
Definition
|
|
Term
| why does charcoal work as a separating agent |
|
Definition
| because it can suck up the small particles that are unbound |
|
|
Term
| how do protien precipitants work as a separating agent |
|
Definition
| reacts with antibody in the system causing the free ones to precipitate and free antigens remain in suspension |
|
|
