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Clinical
vet tech
96
Biology
Undergraduate 2
09/22/2008

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Cards

Term
THE CBC INCLUDES WHAT TESTS?
Definition

1) HEMATOCRIT

2) Hb DETERMINATION

3) WBC COUNT

4) RBC COUNT

5) DIFFERENTIAL

Term

The study of blood is called what ?

Definition
Hematology
Term
The main function of blood is to carry oxygen through out the body and get rid of carbon dioxide. what are 6 other function that blood does?
Definition

1) transport nutrient

2) deliver hormones

3) remove waste products

4) regulate body temp

5) regulate pH

6) carry immune system components

Term
The hematocrit tube should be filled how full it order to get accurate results?
Definition
3/4 full
Term

What end of the hematocrit should go into the clay?

Definition
color coded end or the red striped end
Term

What does the PCV  or the packed cell volume tell us ?

Definition

The % of RBC's compared to the other components of the blood

Term

If the animals is dehydrated would the PCV be high or low?

Definition
High - dehydration less water, less plasma, more RBC'S
Term
How long do you centerfuge a hematocrit tube?
Definition
5 min
Term
What information can be obtain from a hematocrit test?
Definition

PCV

 

Est. RBC Count

 

Est. HB

 

Mircofilaria

Term
what is the formula for the est. RBC?
Definition

(PVC = P)

 

(P/6) x 1,000,000 = RBC estimate

Term
What is the formula for the esitmate of Hb?
Definition

(PCV = P)

 

(P/3) = Hb estimate

Term

What is the purpose of evaluationg the Hb?

Definition

To assess the blood's capability of transporting oxygen to the tissue.

Term
Where on the hematocrit tube do you look for mircfilaria?
Definition
just above the buffy coat
Term
What power and light amount do you use to observe microfilaria?
Definition
low light and low power
Term
What is mircofilaria?
Definition
Larval Heartworm
Term

PLASMA COLOR

1) STRAW COLORED

 

2) YELLOW

 

 3) WHITE OR CLOUDY

 

4) PALE PINK

5) ORANGE

Definition

1) NORMAL FOR CATS AND DOGS

 

2) NORMAL FOR HORSE ONLY - ABNORMAL (ICTERIC) FOR CATS AND DOGS

 

3)LIPEMIC

 

4) HEMOLYZED

5) HEMOLYZED & ICTERIC

Term
What does the TP tell us?
Definition
the protien plasma concentration
Term
If the animal is dehydrated the TP will be high or low?
Definition
High
Term
If the animal is not eating with the TP be high or low?
Definition
LOW
Term
A large buffy coat indicates what ?
Definition
An increase in either the WBC count or the platelets
Term
How many mircoliters does the unopette pipette hold?
Definition
20
Term
What power and light amount do you use to observe the WBC'S on the hemocytometer?
Definition
LOW POWER AND LOW LIGHT
Term

What is the formula for the WBC count?

Definition
(W x 1.1) x 100 = wbc count
Term

What is the diluent used to lyse the RBC's?

 

and what is the dilution ratio?

Definition

1% buffered ammonium oxalate

 

and 1:100

Term
what is the purpose of the wbc count?
Definition
determine the total number of wbc's in 1 ul of blood
Term

An increase in the WBC count may indcate what kind of infection?

 

and a decrease?

Definition

bacterial

 

and

 

viral

Term
what power and light amount do you use to look at platelets on the hemocytometer?
Definition

high power and low light

Term
what is the formula to calculate the number of platelets / ul on the hemocytometer?
Definition
p x 1000 = # of platelets
Term
what decimal place do you round HB to?
Definition
tenth
Term
What is the purpose of the differential?
Definition
shows changes in number and distribution of luekocytes during the disease process
Term
what is EDTA?
Definition
Anitcoagulant that will not change cell morphology
Term
what objective and amount of light do you use to look at the differential?
Definition
high power (oil immersion) and high light amount
Term
how many leukocytes should be count for each 10,000 wbc's counted during the wbc count
Definition
100
Term
What are the 5 types of white blood cells?
Definition
monocyte, lymphocyte, eosinophil, basophil, and segmented neutophil
Term

WHAT IS THE MATURATION SEQUENCE FOR WBCS?

Definition

STEM CELL

MYELOCYTE

PROMYELOCYTE

N,E,B MYELOCTE

N,E,B META MYELOCYTE

N,E,B BAND CELL

MATURE N,E,B

Term
FUNCTION OF THE SEGMENTED NEUTROPHIL?
Definition
PHAGOCYTIZE SMALL PARTICLES
Term
A separate appenedage off the nucleus, made of nuclear material. seen in wbc of females
Definition
barr bodies
Term
 neutrophil that is not well defined or undersegmented in called?
Definition
hyposegmented
Term
 A neutorphil with more than 5 lobes?
Definition
hyposegmented
Term
small round bluish body in a neutrophil's cytoplasm that my indicate toxcity?
Definition

dohle body

Term
what are the charateristics of a toxic neutrophil?
Definition

1) dohle bodies

2) prominant granulation

3) cytoplasm basophilic apperance

4) cell swells

5) vacuoles

6) cell may lyse

Term
another name for a band cell?
Definition
stab cell
Term
increase in neutrophils?
Definition
neutrophilia
Term

Increase in eosinophils?

 

decrease?

Definition

eosinophilia

 

and

 

eosinopenia

Term
what is the primary function of an eosinophil?
Definition
detoxifier
Term
wbc capable of removing histamine from tissues?
Definition
eosinophil
Term
what wbc contains heparin and histamine?
Definition
basophil
Term
What is the maturation sequence for lymphocytes?
Definition

lymphoblast

 

prolymphocyte

 

mature lymphocyte

Term
where are lymphocytes primarly formed?
Definition
spleen, lymph nodes, and bone marrow
Term
where are granulocytes primarily formed?
Definition
bone marrow
Term
What is the primary function of the lymphocyte?
Definition
antigen/ antibody response
Term
lymphoctyosis
Definition
increase in lymphocytes
Term
lymphopenia
Definition
decrease in lymphocytes
Term
maturation sequence for monocytes?
Definition

monoblast

 

promonocyte

 

mature monocyte

Term
what is the primary function of a monocyte?
Definition
phagocytize large particles
Term
monocytosis
Definition
increase in monocytes
Term

term for increase in wbc's?

Definition
leukocytosis
Term
term for decrease in wbc's?
Definition
leukopenia
Term
increase in immature cells in the granulocyte series?
Definition
"shift to the left"
Term

elevated wbc count and and increase in immature granulocytes?

Definition

"regenerative shift to the left"

Term
low leukoctye count witn and increase in immature granulocytes
Definition
"degenerative shift to the left"
Term
increase in the presence of older cells
Definition

"shift to the right"

Term
of unknown cause
Definition
idopathic
Term

what is the formula for calculation absoulte values?

 

Definition
% of WBC type x total WBC count = absolute value
Term
per oil immersion field how many platelets are there on average?
Definition
10 -30
Term

What is the priciple factor for stimulation of RBC production?

Definition
Erythropoietin - hormone produced in the kidneys
Term
monocytes and erythrocytes are both commonlt formed where?
Definition
bone marrow
Term
in order for rbc to be produced in the body what must be present?
Definition

1) protien globulin

2) iron, cobalt, copper, and b vitamins

3)erythropoietin

Term
what is the maturation sequence for RBC's
Definition

stem cell

rubriblast

prorubricyte

rubricyte

metarubricyte

reticulocyte (no nucleus)

mature rbc (no nucleus)

 

Term
each rubriblast can give rise to how many mature cells ?
Definition
6 -18
Term
what is the life span of an erythrocyte?
Definition
62 -160 days
Term
What is the formula for correcting the WBC count?
Definition
(100 wbc counted on diff/ 100 wbc counted on diff + nrbc) x wbc on unopette
Term
IMPLIES NORMAL CELL SIZE
Definition
NORMOCYTIC
Term
IMPLIES NORMAL CONCENTRATION OF Hb (RBC COLOR)
Definition
NORMOCHROMATIC
Term

variation in rbc size

Definition
anisocytosis
Term

 

*macrocyte

 

*microcyte

Definition

 

*larger than  normal

 

*smaller than normal

Term
abnormal deviation in cell shaped (rbc)
Definition
poikilocytosis
Term
stained center of rbc surrounded by and unstained area. surround by on the outside by a stain peripheral ring of cytoplasm.
Definition
codocyte or target cell
Term
rbc with no center pallor - perfectly round
Definition
spherocyte
Term
oval shaped rbc
Definition
ovalocyte
Term
fragmented rbc's
Definition
schistocytes
Term
helmet shaped rbc
Definition
keratocyte
Term
what are the two types of spiculated rbc's and how are the different from one another
Definition

1) acanthocyte - irregular spine like projections

 

2) echinocytes- regular spine like projections

Term
tear drop shaped rbcs
Definition
dracocytes
Term
rbc's with an oval shape that has a linear area which is lighter. resemble a pair of lips or stoma.
Definition
stomatocytes
Term
rbc's that are lighter than normal or have less Hb
Definition
hypochromic
Term
hyperchomic
Definition

rbc's that are darker in color. Have more Hb than normal

Term
variation in rbc color
Definition
polychromasia or anisochromasia
Term
blue staining granules in rbc's the size of pen dots
Definition
basophilic stippling
Term

RBC's with a thin membrane extending around from a helmet shaped cell

Definition
blister cell
Term
a nucleated RBC is called what ?
Definition
metarubricyte
Term
A large blue stainning rbc that may be a reticuloctye
Definition
basophilic macrocyte
Term
remnants of nuclear material in rbc's. appear as dark blue dots (look like dohle bodies) not uncommon
Definition
howell - jolly bodies
Term
denature Hb. appear as an extra appendage of the cell.
Definition
heinz body
Term
rbc's in rows of colunms. common in horses - chains of rbc's
Definition
rouleaux formation
Term
the clumping of rbc'c
Definition
agglutination
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