Term
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Definition
using UV radiation, some substances absorb the radiation and get excited
after excitation they return to ground state and emit a photon of light
fluorescent light results from absorbace of the photon of radiant energy |
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Term
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Definition
| lamp --> entrance slit --> primary monochromater --> exit slit --> sample --> entrance slit --> secondary monochromater --> exit slit --> photo detector --> read out |
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Term
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Definition
gas discharge (mercury or xenon)
incandescent lamps can't be used as they release little UV light |
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Term
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Definition
made of quartz as it is transparent to UV wavelengths
fluorescing sample emits light in all directions |
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Term
Fluorometry - Primary Filter
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Definition
| selects the wavelength that is best absorbed by the solution to be measured |
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Term
Fluorometry - Secondary Filter
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Definition
placed at right angle to sample cell so it can pass longer wavelengths of fluorescent light and prevent incident light from hitting photodetector
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Term
Fluorometry - Monochromators
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Definition
gratings
prisms
filters for isolation of radiation |
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Term
Fluorometry - Photodetector
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Definition
| photomultiplier tubes because of their higher sensitivity to low light intensities |
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Term
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Definition
| the difference in wavelengths between the band maximas of the absorption spectra and the emission spectra |
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