Term
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Definition
| Classic method for quantitating total protein |
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Term
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Definition
| Total Nitrogen Content x 16% |
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Term
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Definition
| Routine method for quantitating total protein |
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Term
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Definition
Cupric ions + peptide bonds = Violet
Required 2 or more peptide bonds and an alkaline medium
Color is proportional to protein concentration |
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Term
| For Biuret you should read color on a spec at ____ nm |
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Definition
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Term
| Should you avoid hemolysis with Biuret |
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Definition
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Term
| Dye-Binding is often associated with which type of substance? |
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Definition
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Term
| Albumin + dye absorbs light at about _____ nm |
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Definition
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Term
True or False:
Absorbance is directly proportional to albumin concentration with Dye-Binding testing method |
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Definition
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Term
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Definition
| Positive charged Albumin + Negative charged dye |
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Term
| What types of dyes are associated with Dye-Binding? |
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Definition
Bromcresol green
Bromcresol purple
Methyl Orange |
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Term
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Definition
Serum proteins added to specific antibody
Detectors measure light scatter caused by ag-ab complexes
Amount of light is proportional to the protein's concentration |
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Term
| What substances are often associated with Nephelometry |
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Definition
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Term
| Is Serum Protein Eelectrophoresis quantitative or semi-quantitative? |
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Definition
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Term
| With the SPE in what direction will negatively charged proteins travel? |
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Definition
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Term
| What does the rate of migration with the SPE depend on? |
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Definition
Size
Charge
Shape
Time
Support Media
Endosmosis |
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Term
| What are the support media associated with SPE? |
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Definition
Cellulose acetate
Agarose gel
Polyacrylamide gel |
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Term
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Definition
Ponceau S
Amido Black
Coomassie Blue |
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Term
| A _____ converts visible protein bands into peaks. The area under each peak can be semi-quantitated. |
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Alpha 1-Antitrypsin deficiency |
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Proliferation of one clone of immunoglobulin |
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Definition
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Term
| What diseases are associated with Monoclonal Gammopathy |
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Definition
Multiple Myeloma
Waldenstrom's Macroglobulinemia |
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Term
True or False:
SPE does not identify the specific protein |
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Definition
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Term
| What test would you run after SPE? |
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Definition
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Term
True or False:
M spike may appear in alpha 2, beta, or gamma regions |
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Definition
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Term
| What type of SPE are Osteolytic lesions and elevated calcium often associated with? |
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Definition
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Term
| Increase in all gamma proteins |
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Definition
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Term
| What illnesses are often associated with Polyclonal Gammopathy |
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Definition
Chronic inflammation
Infections |
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Term
| Hypogammaglobulinemia is associated with what type of illness? |
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Definition
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Term
| Is Hypogammaglobulinemia inherited or acquired? |
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Definition
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Term
| How can Hypogammaglobulinemia be acquired? |
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Definition
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Term
| What illness is associated with Alpha 1-Antitrypsin deficiency? |
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Definition
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Term
| Beta-gamma bridging is associated with what type of gamma proteins? |
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Definition
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Term
| What illness is Beta-gamma bridging associated with? |
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Definition
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Term
| In Nephrotic syndrome what substance is associated with increased protein loss through the kidneys? |
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Definition
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Term
| In Nephrotic syndrome what substance is associated with decreased protein loss through the kidneys? |
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
| Too many globulins or Too little albumin |
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Term
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Definition
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Term
| What illnesses are associated with: low A/G ratio due to too many globulins |
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Definition
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Term
| What illnesses are associated with: low A/G ratio due to too little albumin |
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Definition
Renal disease
Liver disease |
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Term
| What illnesses are associated with: not enough globulins |
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Definition
Genetic
Leukemia
Immunosuppressed |
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Term
| Immunofixation Electrophoresis |
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Definition
| Identifies the specific protein causing the M spike on SPE or UPE |
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Term
| Normal Urine protein electrophoresis |
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Definition
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Term
| Normal Urine protein electrophoresis shows only a _____ amount of albumin |
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Definition
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Term
| Urine with Bence Jones Protein |
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Definition
Obvious M Spike
IFE needed to identify type of light chain present |
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Term
| What type of band is more obvious with a Normal CSF Electrophoresis? |
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Definition
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Term
| CSF with Oligoclonal Bands |
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Definition
Abnormal banding in the gamma region
IgG Index >0.8 |
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Term
| What disease is CSF with Oligoclonal Bands consistent with? |
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Definition
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Term
| Which type of Electrophoresis is fast and requires little sample? |
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Definition
| Capillary (Zone) Electrophoresis |
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Term
| With Capillary Electrophoresis, when the capillary is connected to a power supply, the charged molecules migrate at different rates depending on what? |
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Definition
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Term
| Capillary Electrophoresis: ____ are measured by absorbance as they pass through a detector |
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Definition
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Term
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Definition
| Non-specific measure of protein changes due to inflammation/tissue damage |
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Term
| Does Plasma Viscosity Increase or Decrease in parallel to the ESR |
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Definition
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Term
| Is Plasma Viscosity affected by hematocrit or testing delays? |
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Definition
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Term
| What diseases is an increased plasma viscosity associated with? |
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Definition
Myeloma
Hyperfibrinogenemia |
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Term
| How is Plasma Viscosity measured with? |
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Definition
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Term
| What are the reporting units of Plasma Viscosity? |
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Definition
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Term
| What is the Normal Rate of Plasma Viscosity? |
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Definition
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Term
| Water at Room Temperature is about ___ cP |
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Definition
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Term
| What is the Critical Value for Plasma Viscosity? |
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Definition
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Term
| If G is increased or A is decreased the ratio will be low. What 3 diseases are associated with this? |
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Definition
MM
Chronic Disease
Nephrotic Syndrome |
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Term
| If G is decreased the ratio will be high. What disease is associated with this? |
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Definition
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