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classification and identification
bio-119 ch.10
17
Microbiology
Undergraduate 1
10/24/2013

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Cards

Term

Taxonomy

Definition
study of organisms to arrange them into groups for purposes of classification and identification
Term
Identification
Definition
identify a particular unknown (one organism)
Term
classification
Definition
who is related to who (family tree)
Term
prokaryotic species
Definition
a group of genetically related cells with the same core genetics
Term
taxonomic hierarchy
Definition
  • domain
  • kingdom (eukaryotes only)
  • phylum or division
  • class
  • order
  • family
  • genus (more broad)
  • species (+/- strains) 
Term
Three domain classification sysytem
Definition
Bacteria, Archaea, Eukarya
Term

where did all cells arise from?

 

Definition
prokaryotic ancestor
Term
phenotype
Definition
appearance and functioning
Term

microscopic morphology

(phenotype)

Definition
  • size and shape
  • gram and special stain 
Term

colony morphology

(phenotype)

Definition
  • unique appearance, pigments
  • may require differential media
Term

metabolic testing

(phenotype)

Definition
  • enzymes
  • groups of enzymes in pathways
Term
dichotomous key
Definition
lays out the test and helps one walk through the different test with their results to narrow down to one thing
Term
enterotube
Definition
has chambers of media and innoculates everything at once
Term
Steps of phenotype
Definition
  • microscopic morphology
  • colony morphology
  • metabolic testing
  • serology
  • other analyses
Term
2 ways for identification of prokaryotes
Definition

phenotype

genotype

Term
steps of genotype
Definition
  • sequencing rRNA/rDNA
  • DNA fingerprinting (RFLP, PCR)
  • DNA probes (colony blotting, FISH, DNA microarray)
Term
why is rRNA preferred?
Definition
it doesnt change over time because it is ribosomal. it is conserved.
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