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Chemistry Laboratory 1
Osmometry
16
Chemistry
Graduate
11/29/2012

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Term
The osmolality of a urine sample or serum specimen is measured by a change in the
Definition
freezing point
Term
Osmolal gap is
Definition
the difference between the calculated and measured osmolality value
Term
The formula for calculating serum osmolality that incorporates a correction for the water content of plasma is
Definition
(2 Na + Glucose / 18) + (BUN/2.8)
Term
The osmolal gap is defined as measured Osm/kg minus the calculated Osm/kg. The average osmolal gap is near
Definition
zero
Term
The solute that contributes the most to the total serum osmolality is
Definition
sodium
Term
An adult diabetic with renal complications has the following results. calculate theoretical osmolality
Sodium = 133 mEq/L
BUN = 84mg/dL
Glucose = 486 mg/dL
Creatinine = 5 mg/dL
Definition
(2 Na + Glucose)/ 18 + BUN/2.8
2 (144) + 486/18 + 84/2.8
323 mOsm/kg
Term
A patient had the following serum results, calculate osmolality:
Sodium = 140 mEq/L
Glucose = 95 mg/dL
BUN = 10 mg/dL
Definition
2(Na) + glucose/18 + BUN/2.8
2(140) + 95/18 + 10/2.8
289 mOsm/kg
Term
List the 4 colligative properties of solution
Definition
Osmotic pressure, decreased vapor pressure, decreased freezing point, and increased boiling point
Term
One mole (Avagadro's number of molecules) of non-ionizing solute dissolved in one kilogram of water will ________ the freezing point by ______degrees C.
Definition
Decrease, 1.86
Term
The concentrating abilities of the kidney are the best evaluated by
Definition
osmolality
Term
The osmolality of a solution is dependent upon
Definition
the number of particles
Term
An increase in the osmolar gap may often be associated with
Definition
ingestion of toxic substances, ingestion of alcohol, presence of ketones
Term
List the four primary constituents found in serum that are measured by osmometry
Definition
Na+, Cl-. Glucose and BUN (urea)
Term
Define osmometry
Definition
techniques to measure the concentration of the dissolved solute particles in solution that contributes to osmotic pressure
Term
When referring to osmolality, what are the units measured?
Definition
mOsm/ kg
Term
Explain the difference between osmolarity and osmolality
Definition
Both are measurements of concentration of solutes in a solution, but osmolarity is a measurement of the concentration per volume, which is temperature dependent. Osmolality is concentration relative to mass of solution and is temperature independent
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