Shared Flashcard Set

Details

Chapter Eight (quiz four)
Microbial Metabolism
90
Microbiology
Undergraduate 2
02/23/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Metabolism
Definition
all chemical reactions and physical work of the cell
Term
Functions of Metabolism
Definition
1. Assembles smaller molecules into larger macro-molecules needed for the cell
2. Degrades macro-molecules into smaller molecules and yields energy
3. Energy is conserved into the form of ATP or HEAT
Term
Anabolism (biosynthesis)
Definition
Any process that results in the synthesis of cell molecules and structures (usually requires energy input)
-making new molecule from existing parts
Term
Catabolism
Definition
the breakdown of bonds of larger molecules into smaller molecules (often releases energy)
Term
Enzymes?
Definition
are proteins that work as catalysts
Term
Catalysts
Definition
chemicals that increas the rate of a chemical reaction without becoming part of the products of being consumed in the reaction
Term
What do catalysts do to activation engery
Definition
lower it
Term
What is unique about each catalyst?
Definition
unique characteristics such as shape, specificity and function
Term
Substrates?
Definition
target molecule; each enzyme has a active site for these
Term
What are enzymes greatly effected by
Definition
temperature and pH
Term
1st enzyme in glycolysis?
Definition
Hexokinase
Term
Energy of Activation
Definition
the amount of energy which must be overcome for a reaction to proceed
Term
How do enzymes promote a reaction?
Definition
by serving as a PHYSICAL SITE upon which the reactant molecules (substrates) can be positioned for various interactions
Term
Enzyme-substrate interaction
Definition
-for a reaction to take place, a temporary enzyme-substrate union must occur at the active site
-"lock and key" fit
-these bond are weak and easily reversible
Term
What is an ES complex?
Definition
the enzyme and substrate bound together
Term
What are cofactors?
Definition
supporting the work of enzymes
Term
Metallic cofactors?
Definition
activate enzymess, help bring the active site and substrate close together, and participate directly in chemical reaction with the enzyme-substrate complex
Term
Coenzymes are?
Definition
Organic compounds that work in conjunction with a apoenzyme to preform a necessary alteration of a substrate
Term
Coenzymes do?
Definition
remove a chemical group from one substrate molecule and adds it to another substrate
Term
What is one of the most important components of coenzymes?
Definition
VITAMINS
Term
Holoenzyme?
Definition
apoenzyme (protein enzyme) + co-factor = holoenzyme = catalyzes actual function/reaction
Term
Classification of enzymes? (3 ways)
Definition
1. Site of action
2. type of action
3. substrate in which they work with
Term
hydrolase (enzyme)
Definition
breaks down lactose down into glucose and galatose
AND
hydrolyzes beta-lactam ring
Term
transferase (enzyme)
Definition
synthesizes a strand of DNA using the complementary strand as a model
Term
oxidoreuctase (enzyme)
Definition
catalyzes the conversion of pyuvric acid to lactic acid
AND
catalyzes the reduction 02
Term
Constitutive Enzymes?
Definition
always present and in relatively constant amounts
(ex. enzymes of glycolysis)
Term
Regulated enzymes?
Definition
Production is either induced or repressed in response to a change in the concentration of the substrate
(ex. lactose (lac) operon in E. coli)
Term
Lac operon in E. coli?
Definition
the E. coli n the presence of lactose turns on its enzymes to break down lactose... regulated enzyme you fool!
Term
Dehydration recation
Definition
water is one of the products
Term
Hydrolysis
Definition
breaking down peptide bonds between amino acids. requires a water molecule that adds H and OH to the amino acids
Term
Virulence factors or toxins
Definition
-unique exoenzymes that pathogens secrete
-these help them avoid host defenses or promote multiplication in tissues
Term
Labile enzymes?
Definition
when enzymes are subjected to changes in normal conditions they become unstable or labile
Term
Denaturation?
Definition
the weak bond that maintain the native shape of the apoenzyme are broken
Term
Enzymes that play a role in necessary molecular conversions by directing the transfer of function groups
Definition
-aminotransferases
-phosphotransferases
-methyltransferases
-decarboxylases
Term
How do metabolic reactions usually occur?
Definition
in a multi-series step or pathway
-each step is catalyzed by an enzyme
-each pathway has one or more enzyme pacemakers that set the rate of a pathway's progression
Term
What are intermedates in a metabolic pathway?
Definition
Not the start or finished product... DUH
Term
Competitive inhibition?
Definition
It is a direct control on the action of enzymes
-the cell supplies a molecule that resembles the enzyme's normal substrate, which then occupies and blocks the enzyme's active site
Term
Non-Competitive inhibition?
Definition
direction controls on the action of enzymes
-the enzymes has TWO binding sites- the active site and the regulatory site; a regulator molecule binds to the regulatory site providing a negative feed back mechanism
Term
Enzyme Repression?
Definition
Decrease enzyme expression (product goes back to DNA and says "ENOUGH!")
Term
Enzyme Induction?
Definition
Increase enzyme expression (product goes back to DNA and says "MORE MORE")
Term
Exergonic Reactions?
Definition
a reaction that releases energy as it goes forward: -delta G
usually catobolic RXNS
Term
Engergonic Reactions?
Definition
a reaction that is driven forward with the addition of energy: +delta G
usually anabolic RXNS
Term
Redox reaction
Definition
how biological systems extract energy, always occur in pairs (an electron donor paired with an electron acceptor
Term
Electron Carriers
Definition
Repeatedly accept and release electrons and hydrogen
-some are coenzymes that transfer electrons and hyrogens
-ex. NAD, FAD, NADP
Term
ATP
Definition
adenosine triphosphate
-Metabolic Money
Term
ATP molecular structure
Definition
-nitrogen base
-5 carbon sugar
-chain of three phosphate group
Term
How does ATP yield energy
Definition
breaking of bonds between two phosphates of ATP yeilds ADP plus the inorganic phosphate
-the most energy comes from breaking the 3rd group
Term
how much energy comes from ATP
Definition
-7.3 kcal/mol
Term
Substrate Level Phosphorylation
Definition
phosphate off substrate to ATP OR the transfer of phosphate group
- phosphoenolpyruvate+ADP -> pyruvate+ATP
Term
What is a pathway?
Definition
a series of biochemical reactions
Term
what is often the nutrient going through catabolism?
Definition
glucose!
Term
What are the three major catabolism pathways?
Definition
1. Aerobic respiration
2. Fermentation
3. Anaerobic Respiration
Term
Aerobic Respiration?
Definition
series of reactions that convert glucose to CO2 and allows the cell to recover significant amounts of energy; requires OXYGEN (makes water)
Term
Fermentation?
Definition
use only glycolysis to incompletely oxidize glucose
Term
Anaerobic respiration?
Definition
does not use molecular oxygen as the final acceptor
Term
Anaerobic random ifo
Definition
-its a series of enzyme-catalyzed reactions
-electrons are transfered from fuel molecules to oxygen as a final electron acceptor
-provides ATP and metabolic intermediates for many other pathways
-glucose is the starting compound
Term
What does glycolysis convert glucose to?
Definition
it goes through several steps into pyruvic acid OR pyruvavte
Term
3 phases of glycolsis
Definition
1st phase: 2 ATP taken in
2nd phase: 2 three carbon molecules made
3rd phase: making 4 ATP and 2 NADH
Term
Products of Glycolysis
Definition
-2 pyruvate molecules (can under go more catabolic RNX to extract more energy)
-2 ATP
-2 NADH
Term
What does Glycolysis convert glucose into?
Definition
Pyruvate
Term
3 phases of pyruvate
Definition
1st: 2 atp taken in
2nd: 2 three carbon molecules made
3rd: making 4 atp and 2 nadh
Term
Products of Glycolysis
Definition
2 pyruvate molecules
2 ATP
2 NADH
Term
What does pyruvic acid serve as?
Definition
an important position in several pathways
Term
Krebs Cycle take place where?
Definition
in the cytoplasm of bacteria and mitochondrial matrix in eukaryotes
Term
Products of krebs cycle?
Definition
1 glucose molecule makes 2 pyruvate
each pyruvate makes: 3 CO2, 4 NADH, 1 FADH2, 1 ATP
so total
8 NADH
2 FADH2
2 ATP
Term
After Krebs cycle what is up with glucose?
Definition
It has now be completely oxidized to carbon dioxide
Term
What is the final processing mill
Definition
electron transport chain
Term
What do electrons pass through in the electron transport chain?
Definition
membrane carries called CYTOCHROMES
Term
How is atp formed in electron transport chain
Definition
the protons pass through ATP synthase to form ATP

this is an endergonic reaction-- energy comes from protons moving down the gradient
Term
1 FADH2 = _____ ATP
Definition
2
Term
1 NADH = ____ ATP
Definition
3
Term
Aerobic respiration yields....?
Definition
36-38 ATP per gulcose
Term
Anaerobic respiration
Definition
functions like aerobic system except it utilizes oxygen-containing ions rather than free oxygen as the final electron acceptor
Term
Anaerobic Respiration yield?
Definition
2-36 ATP
Term
Fermentation is..?
Definition
the incomplete oxidation of glucose or other carbohydrates in the absence of oxygen
Term
What does fermentation use as the terminal electron acceptor
Definition
organic compounds
Term
How are bacteria able to grow as fast using fermentation as they would in the presence of O2
Definition
they increase the rate of glycolysis.. the 2 ATP build up
Term
Products of fermentation?
Definition
alcoholic beverages, organic acids, dairy products, vitamins, antobiotics
Term
Two general categories of fermentation?
Definition
1. Alcoholic fermentation
2. Acidic Fermentation
Term
Commonality between Alcoholic and Acidic Fermentation
Definition
NADH getting reformed into NAD... this allows glycolysis to continue
Term
Alcoholic fermentation products
Definition
ethanol, CO2 and NAD
Term
Acidic Fermentation products
Definition
varied... but always lactic acid
Term
lactic acid bacteria fermentation product
Definition
they ferment pyruvate and reduce it to lactic acid (occurs in our muscles)
Term
Heterolactic fermentation
Definition
when glucose is fermented to a mixture of lactic acid, acetic acid, carbon dioxide and NAD
Term
Mixed acid fermentation
Definition
Produced a combination of acetic, lactic, succinic and formic acids and lowers the pH of a medium to about 4
Term
homolactic bacteria?
Definition
S. coccus or latobacilus form lacti acid... souring of milk
Term
Amphibolism
Definition
The property of a system to integrate catabolic and anabolic pathways to improve cell efficiency
Term
where do monosaccharides, amino acids, fatty acids, nitrogen bases and vitamins come from (in regards to anabolism)
Definition
1. enter the cell from outside as nutrients
2. Can be synthesized through various cellular pathways
Term
Amino acids, protein synthesis and nucleic acid synthesis?
Definition
some organisms can synthesize all 20 amino acids
other organisms (especially animals) must acquire the essential ones from their diets
Term
Assembly of the cell
Definition
when anabolism produces enough macro-molecules to serve two cells, then the cell undergoes binary fisson
Supporting users have an ad free experience!