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Chapter 9 Questions
Chapter 9 questions
45
Microbiology
Undergraduate 2
11/02/2016

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Term
Can you name the 4 concepts in antimicrobial control?
Definition
Sterilization
Disinfection
Decontamination/Sanitization
Antisepsis/Degermation
Term
Name an example of sterilization
Definition
Heat via autoclave
Term
Which out of the 4 antimicrobial concepts are used on inanimate objects?
Definition
Sterilization
Disinfection
Decontamination/Sanitization
Term
Give an example of an agent used to disinfect an inanimate object?
Definition
Bleach
Term
Which out of the 4 antimicrobial concepts in essentially important for restaurants, breweries,
Definition
Decontamination
Term
Name 3 examples that would decontaminate
Definition
Detergents, soaps, and commercial dishwahsers
Term
Which method of antimicrobial control is used on reusable objects like utensils and dishware?
Definition
Decontamination
Term
Which method of antimicrobial control is the only one safe for use on human skin?
Definition
Antisepsis
Term
Name 2 examples of antisepsis agents
Definition
Alcohol
Surgical hand scrub
Term
Name the 3 (broad) microbial control methods
Definition
Physical
Chemical
Mechanical
Term
Antisepsis on animate objects belongs to which method: physical, chemical or mechanical?
Definition
Chemical...under liquids
Term
Name two agents that belong to the physical category of microbial control methods
Definition
Heat and Radiation
Term
What are the two types of heat?
Definition
Dry and moist
Term
Steam under pressure, boiling water and pasteurization are examples of which type of heat?
Definition
Moist
Term
Incineration and dry oven are examples of which type of heat?
Definition
Dry
Term
Name a few examples of radiation?
Definition
Xray, gamma ray, UV
Term
What is the goal of any sterilization process?
Definition
To kill endospores
Term
Compare Fungicide to Fungistatic.....which will kill fungal spores?
Definition
Fungicide
Term
In terms of death rate of a microbe population, which out of the two would take longer to die?
1. a pure sample of one type of bacteria
2. a plate that contains many types of bacteria due to contamination
Definition
#2 Higher loads of contaminates take longer to kill off
Term
Name the 4 cellular targets that physical and chemical agents aim to reach?
Definition
1. cell wall
2. cell membrane
3. cellular synthetic process
4. proteins
Term
When an agent attacks the cell wall, what are the two things they are doing to kill the cell at this location?
And name two examples of which agents can do this?
Definition
At the cell wall they are:
1. Blocking the synthesis
2. Digesting the wall

Chemicals, detergents and alcohol can do this
Term
When an agent attacks the cytoplasmic membrane, what is it actually doing to the cytoplasmic membrane?

Name some agents that can do this
Definition
Agents physically bind the lipid bilayer, opening up the cytoplasmic membrane allowing the agent in and allows important ions to exit the cell

Detergents can do this
Term
How can agents interrupt cellular synthesis?

Name some agents that can do this
Definition
Agents interrupt the process of synthesis via the ribosomes. Without the ribosomes the cell cannot make essential proteins needs to grow, multiply and metabolize

Radiation and Formaldehyde can do this
Term
Which two ways are proteins disturbed by agents?
Definition
It can denature the proteins.

It can also block the active sites for protein. That prevents it from reacting with its chemical substrate
Term
Name two agents or examples that affect proteins?
Definition
Moist heat and alcohol
Term
In terms of microbicidal and microbistatic......which is found at lower temps and which is at higher?
Definition
Elevated temps = microbicidal

Lower temps= microbistatic

If it's hard to remember, cidal means ability to kill. People who are homicidal probably have elevated temperaments....they are angry! Hot headed!
Term
Which heat dehydrates the cell?
Definition
Dry
Term
Which heat can denature proteins to make them more resistant?
Definition
Dry
Term
Which heat denatures of proteins?
Definition
Dry
Term
Which heat has a microbicidal effect of coagulation and denaturation of proteins
Definition
Moist
Term
Which heat operates at lower temperatures but shorter exposure times?
Definition
Moist
Term
While boiling water kill endospores?
Definition
Nope, need temps higher than boiling
Term
What is the principle benefit of cold treatment?
Definition
to slow the growth of microbes and cultures in food during processing and storage
Term
Does cold necessarily kill fungus, spores, viruses and bacteria?
Definition
No, it can actually preserve them
Term
What does desiccation mean?
Definition
extreme drying
Term
What term is used to describe a combination of freezing and drying?
Definition
Lyophilization
Term
Which process is described: Used to prepare liquids that cannot withstand heat such as serum, blood products, vaccines, drugs, IV fluids, enzymes, and media
Definition
Filtration
Term
Which method describes these:
Alternative method for decontaminating milk and beer without altering its flavor

Important step in water purification
Unable to remove soluble molecules (toxins) that can cause disease

High efficiency particulate air (HEPA) filters are used in hospital rooms and sterile rooms.
Definition
Filtration
Term
So, if you were stranded in the woods and you came upon a freshly dead deer, which an abundance of meat on the carcass. How could you preserve the meat to safely eat it?
Definition
You could smoke it! Like make deer jerky!
Term
Adding large amounts of salt or sugar to foods create a hypertonic environment, causing what to happen?
Definition
Plasmolysis (ie. cells lose water)
Term
Pickling, smoking and drying foods do what to them?
Definition
Preserve them
Term
Is osmotic pressure a sterilization technique?
Definition
NEVER!
Term
Which agent is an ideal one, meaning that it can almost do all of these:
Rapid action, even in low conc.
Has selective toxicity
Resistant to organic matter
Noncorrosive and Nonstaining
Sanitizing and deodorizing
Affordable and readily available
Definition
Hydrogen peroxide
Term
Which level of germicides kill endospores?

High, Intermediate or Low
Definition
High levels
Term
What kind of agent is effective against GP bacteria and Fungi but is limited in application because it stains and has a narrow range of activity
Definition
Dyes
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