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Chapter 9
Powerpoint
28
Microbiology
Undergraduate 1
11/17/2010

Additional Microbiology Flashcards

 


 

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Term
vertically and horizontally
Definition
Genetic Information in prokaryotes can be transferred ___ and ____.
Term
Vertical Gene Transfer (VGT)
Definition
the transfer of genetic material from parent cell to daughter cell
Term
Horizontal Gene Transfer (HGT)
Definition
the transfer of DNA from a donor cell to a recipient cell
Term
Transformation
Definition
the uptake and expression of DNA in a recipient cell, by the integration of a new DNA fragment, the recipient has gained some ability it previously lacked
Term
Competence
Definition
the ability of a recipient cell to take up DNA from the environment, which may give the recipient the ability to be more pathogenic
Term
Conjugation
Definition
involves cell-to-cell contact for horizontal gene transfer, in which a donor cell (F+) transfers DNA directly to the recipient (F-), which is done by the donor cell forming a conjugation pilus to make contact with the recipient cell
Term
Rolling Circle Mechanisms
Definition
the F factor DNA in the donor replicates this way
Term
High Frequency Replication (Hfr)
Definition
strains can donate chromosomal genes rather than just the F plasmid
Term
F Factor
Definition
attaches to the chromosome using an insertion sequence
Term
Recombinant F-
Definition
When conjugation is usually interrupted before the entire chromosome is transferred. The recipient remains F- called..
Term
F plasmid
Definition
If an integrated F plasmid breaks from the chromosome taking a fragment of chromosomal DNA
Term
Transduction
Definition
involved viruses as agents for horizontal transfer of DNA, a virus (Bacteriophage) carries a chromosomal DNA fragment from donor to recipient
Term
lytic cycle
Definition
virulent phages 1.) destroy the host chromosome 2.) replicate themselves 3.) destroy the cell
Term
lysogenic cycle
Definition
temperate phages integrate their DNA into the host chromosome as a prophage
Term
generalized transduction
Definition
performed by virulent phages, a fragment of host cell DNA ends up in the phage during packaging, which they transfer to a new host cell
Term
Lysogenic Cycle
Definition
the prophage eventually excises itself from the host chromosome, sometimes taking a few nearby host genes and leaving a few phage genes behind
Term
Genetic Engineering
Definition
born from genetic recombination, involves changing the genetic material in an organism to alter its traits or products
Term
Biotechnology
Definition
the commercial and industrial products derived from genetic engineering
Term
Recombinant DNA molecule
Definition
contains DNA fragments spliced together from 2 or more organisms
Term
restriction endonuclease
Definition
specific fragments can be obtained by cutting short stretches of nucleotides with this
Term
DNA ligase
Definition
joins fragments during recombination of DNA
Term
Genetic Engineering
Definition
The genes responsible for producing human insulin can be cloned into bacteria is an example of...
Term
genetic engineering
Definition
Bacteria can be _____ _____ to break down toxic wastes and produce antibiotics
Term
Plants
Definition
has been engineered using microbial genes for herbicidal activity and viral resistance
Term
bovine growth hormone
Definition
Cows produce more milk when injected with this which is produced by engineered bacteria
Term
DNA probes
Definition
Specific nucleotide sequences in pathogens allows us to identify them using these
Term
DNA probe
Definition
a single strand DNA that recognize and bind with a specific nucleotide sequence of the pathogen
Term
DNA probes
Definition
used to identify HPV DNA in a pap smear
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