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the study of what genes are, how they carry information, how their information is expressed nd how they are replicated and passd to subsequent generations or other organisms |
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a segment of DNA that codes for a functional product, usually a protein |
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all the genetic information in a cell |
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genetic composition of an organisms |
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the expression of the genes; also known as the physical characteristics; the proteins of the cell and the properties they confer on the organism |
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structure containing DNA that carries hereditary information |
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linear (to daughter) or horizontal (to recombinant cell) transfer of information |
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semiconservative replication |
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each parental strand forms with a new strand leaving daughters with parental-new as opposed to one with parental-parental and the other with new-new |
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synthesized discontinuously |
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bidirectional nature of replication |
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replicates on both strands going each way |
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from nothing; which DNA synthesis cannot do |
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relaxes supercoiling ahead of the replication fork |
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makes covalent bonds to join DNA strands; joins okazaki fragments and new segments |
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synthesized DNA; proofreads and repairs DNA |
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cut DNA backbone in a strand of DNA; facilitate repair and insertions |
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cut DNA from an exposed end of DNA; facilitate repair |
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unwinds double-stranded DNA |
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adds methyl group to selected bases in newly made DNA |
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uses visible light energy to separate UV-induced pyrimidine dimers |
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makes RNA primers from a DNA template |
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RNA enzyme that removes introns and splices exons together |
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copies RNA from a DNA template |
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RNA protein complex that removes introns and splices exons together |
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relaxes supercoiling ahead of the replication fork; separates DNA circles at the end of DNA replication |
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cuts DNA backbone leaving single-stranded 'sticky ends' |
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where RNA polymerase binds to to start transcription |
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where the RNA stops making a protein |
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three base segments of mRNA that specify amino acids |
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do not code for amino acids and are stop signals for translation |
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controls the synthesis of one or several repressible enzymes |
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in the presence of certain chemicals (inducers) cells synthesize more enzymes |
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unit of regulation that contains a promoter, regulator and structural genes |
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the P gene where RNA polymerase binds |
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the O gene where regulatory components react |
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when there are two operons being used in a sequence, there is a lag period because it takes time to turn on the second operon; at the promoter site there is a place for CAP whih acts as an activator but need cAMP |
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change in the nitrogenous base sequence of DNA |
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when one base pair in DNA is replaced with a different base pair |
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cause amino acid substitutions |
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one of a few base pairs are deleted or added to DNA |
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agents in the environment that cause permanent changes in DNA |
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occur without the presence of any mutagen |
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examples of cemical mutagens |
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nucleoside analogs and nitrous acid |
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nitrous acid as a mutagen |
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alters adenine so it no longer pairs with thyamine but instead cytosine |
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nucleoside analogues as mutagens |
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pose as nitrogen bases and are incorporated into DNA as adenine and pairs with thyamine; or can look like thyamine and bind with cytosine |
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causes formation of ions that can react with nucleotids and DNA backbone |
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causes bonding between adjacent thymines called thymine dimers |
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positive (direct) selection |
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detects mutant cells because grow or appear different |
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negative (indirect) selection |
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detects mutant cells because they don't grow at all |
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method for isolating mutant cells |
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authentic nonaltered type |
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passed from an organism to its offspring |
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involves a portion of the cells DNA being transferred to a donor recipient |
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genes are transferred from one bacterium to another as naked DNA; discovered by Frederick Griffith with mice killing experiment |
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one type of cell (F+) gives its plasmid to F- cell |
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high frequency plasmid; when the conjugated plasmid becomes incorporated into the chromosome |
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DNA is passed from one bacterium to another by a bacteriophage; the cells DNA is put into the the virus's capsule and transferred to another cell when the viruses lyse; the bacteral DNA is then incorporated into the recipient DNA |
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carries gene for sex pili and transfer of the plasmid |
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encode enzymes for catabolism of unusual compounds |
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encode for antibiotic resistance |
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small segments of DNA that can move from one region to another region of the same chromosome or to a different chromosome or plasmid; they are considered mutagens; they have inverted repeat sequences on either end |
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insertion sequences- can insert themselves between two pieces of DNA |
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can carry any type of chene and are a natural mechanism for moving genes |
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