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Chapter 7
Structure and replication
43
Biology
Not Applicable
05/11/2012

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Term
What does DNA stand for?
Definition
Deoxyribonucleic Acid 
Term
DNA is common to? 
Definition
All living organisms 
Term
DNA is a _______ molecule 
Definition
Hereditary 
Term
Where in the cell is the DNA found?
Definition
in the nucleus of eukaryotic cells 
Term
In what form is DNA found in eukaryotic cells?
Definition
In the form of chromosomes 
Term
WHat does a chromosome consists of? 
Definition
singlel DNA molecules wrapped around protiens called Histones 
Term
How many pairs of chromosomes do humans have?
Definition
23 pairs of chromosmes 
Term
how many total chromosomes do humans have?
Definition
46 total chromosomes 
Term
the 23rd pair of chromosome determines a persons?
Definition
gender 
Term
XX=
Definition
Female 
Term
XY=
Definition
Male 
Term
what shape is DNA? 
Definition
spiral shape double helix(double stranded) 
Term
What is DNA composed of? 
Definition
of nucleotides that are linked togeather 
Term
Each nucleotide consists of?
Definition
a sugar, a phosphate, and a base. 
Term
the sugar and phosphates form the?
Definition
outside "backbone" of the helix 
Term
the bases form?
Definition
the interbal "rungs" like steps on a twisting ladder.
Term
bases are held togeather by? 
Definition
Hydrogen Bonds 
Term
How many diffrent possible nucleotide bases are there? 
Definition
4
Term
What are the different nucletide bases?
Definition

Adenine (A)

Thymine (T)

Guanine (G)

Cytosine (C)

Term
nucleotide bases do not pair up randomly, but rather in a consistent pattern, Which is known as? 
Definition
the complementary base
Term
The complementary base pairing rule is?
Definition

Adenine-----Thymine 

Cytosine-----Guanine

Term
Complementary base pairing is important for?
Definition
DNA replication 
Term
During DNA replication, each complementary strand of DNA serves as a?
Definition
Template 
Term
What heppends during DNA replication?
Definition
the hydrogen bonds that hold base pairs togeather are broken and the helix is unwound.
Term
what is the name of the action that separates the double stranded helix?
Definition
Helicase 
Term
What heppends after the helix is unwound? 
Definition
DNA polymerse adds complementary nucleotides to a new strand. 
Term
DNA polymerase adds complementary nucleotides using what rule of pairing? 
Definition

 base pairing 

 

Adenine----thymine

Guanine----Cytosine 

Term
At the end of DNA replication how many copies of the original DNA molecule exist? 
Definition
2 copies 
Term
DNA replication is said to be? 
Definition
Semi-conservative Replication 
Term
each molecule consists of? 
Definition
an original and a new strand. 
Term
if the sequence of one strand is TGCAG, what would be the sequence of the complementary strand after DNA relpication? ( remember that A pairs with T and C with G)
Definition
the complementary strand after DNA replication would be: ACGTC
Term
If the sequence of one strand of DNA is GTTACGTACGGAT, what is the sequence of the complementary strand after DNA relpication?
Definition

Complementary strand after DNA relpication:  

CAATGCAGGCCTA 

Term

the case of Roy brown, was convicted of murder on faulty and incomplete evidence and exonerated through DNA profiling. 

 

How was brown freed? innocent project, non profit organization founded by two lawyers? 

 

Definition
Barry C. Scheck and Peter J. Neufeld
Term
What did the lawyers use to free brown using? 
Definition
using DNA evidence to free people who have been wrongly convicted of crimes 
Term
Crime scene evidence was obtained by? 
Definition
extracting DNA from saliva and blood cells 
Term
DNA is used to create?
Definition
DNA profile of the perpetrator, a visual representaion of a persons unique DNA sequence. 
Term
When there is not enough crime scene DNA to analyze they use?
Definition
theh polmerase chain reaction technique(PCR)
Term
WHat is (PCR)?
Definition
polymerase chain reaction technique- A chemical reaction that can increase the amount of DNA in a sample.   
Term
Who developed the (PCR) tecnique? 
Definition
Kary Mullis in the mid-1980"s
Term
the (PCR) technique is similar to DNA relpication but occurs in?
Definition
A test tube and only relpicates specific regions of a DNA molecule. 
Term

What needs to be added to PCR to work? 

 

Definition

to a small sample of DNA add-

Nucleotides 

DNA Polymerase Enzyme 

Primers 

Term
How does PCR work? 
Definition
the DNA is first heated to separate strands. -then cooled to allow new nucleotudes to be added by DNA polmerase 
Term
How many copies does PCR make? In how much time 
Definition
billions of copies of a specific DNA region/ in less than a few hours. 
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