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Chapter 6
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34
Biology
Undergraduate 2
07/17/2008

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Term
Microbial growth increases in size or number?
Definition
It increases in number
Term

What are psychotrophs?

Definition

cold loving

grow in 0 degrees to 20 degrees

Causes food spoilage

Term

Most bacteria grow where on the PH scale?

Definition
somewhere from ph 6.5 - 7.5
Term
MOlds and yeast grow between ph:
Definition
ph 5-6
Term
What are acidophiles?
Definition

acid loving

grow in acidic environment

Term
Hypertonic environments increase salt or sugar causes:
Definition
plasmolysis
Term
xtreme or obligate halophiles require what type of osmotic pressure?
Definition
high pressure
Term

What halophile can tolerate high osmotic pressure?

Definition
Facultative halophiles
Term

What are chemical requirements for growth?

Definition

Carbon

Nitrogen

Sulfur

Phosphorus

Term
What are trace elements?
Definition
inorganic elements required in small amounts
Term
Obligate aerobe
Definition
likes oxygen
Term

facultative anerobe

Definition
It can tolerate growth on surface or on bottom
Term
Obligate anerobe
Definition

Doesn't like oxygen

Term
Aerotolerant anerobes
Definition
Can tolerate oxygen
Term
micro aerophiles
Definition
alittle bit of oxygen is tolerated, grows in the middle
Term

Sterile

Definition
No living microbes
Term
A culture medium
Definition
Nutrients prepared for microbial growth
Term
inoculum
Definition
introducing a bacteria into a medium
Term
What is a chemical defined media?
Definition

Exact chemical composition is knowned

Term
Complex media
Definition
Extracts and digest of yeast, meats, plants
Term
What are examples of complex medias?
Definition

Nutrient broth

Nutrient agar

Term
What is a reducing media?
Definition
Contain chemicals that combine O2 and uses heat to drive off O2
Term
What is an anerobic chamber?
Definition
A fully sterilized chamber for contagious or dangerous microbes
Term

A jar that doesn't allow any oxygen in and it holds petri dishes and releases carbondioxide and H2

Definition
anerobic jar
Term
Suppressing unwanted microbes and encouraging desired microbes
Definition
selective media
Term
Type of media that makes it easy to distinguish colonies of different microbes
Definition
differential media
Term
Type of media that encourages growth of desired microbe.
Definition

Enrichment media

Term

At what temperature is deep freezing

Definition
-50 degrees to -90 degrees C
Term

What occurs in lyophilization (freeze-drying)?

Definition
it is frozen to -54 degrees C and then dehydrated by a vacuum
Term

4 reproduction methods of prokaryotes

Definition

binary fission

budding

condiospores

fragmentation of filaments

Term
what are the 4 steps in binary fission?
Definition

1) duplication of DNA

2) DNA divides to 2 sides

3) the cell pinches into 2

4) the cell breaks into 2

Term

What is the life log of cells dying?

Definition
  1. Lag phase
  2. exponential growth phase
  3. stationary phase
  4. death phase
Term
Perform serial dilution of a sample
Definition
Plate counts
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