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Moderate- Temperature Loving Microbes |
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Optimum Growth Temperature |
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is the temperature at which the species grows best. |
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Most bacteria grow best in a narrow PH range near neutrality, between __ and___. |
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Some bacteria called _____, are remarkably tolerant of acidity. |
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Shrinkage of the cell's cytoplasm. |
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The requirements for microbial growth can be divided into 2 categories ____ and _______ |
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Physical and Chemical Physical includes temperature, ph, osmotic pressure Chemical includes carbon nitrogen sulfur oxygen. |
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is the structural backbone of living matter, it is needed for all organic compounds that make up a living cell. One of the most important compounds of living matter |
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Organisms use ------ primarily to form the amino group of the amino acids of proteins. |
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The growth of a population is an increase in the number of |
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Microbes requirse very small amounts of other mineral elements, such as iron, copper, molybdenum and Zinc. |
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Organisms that require oxygen to live are called |
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Organisms that can grow in the abscence of oxygen are called , They can use oxygen when it is present but are able to continue to grow by using fermentation or anaerobic respirations. |
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Bacteria that are unable to use molecular oxygen for energy-yielding reactions. |
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normal molecular oxygen that has been boosted unti a higher energy state and is extremely reactive. |
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Cannot use oxygen for growth, but they can tolerate it fairly well. |
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aerobic, require oxygen, They grow only in oxygen concentrations lower than those in air. grow in very small amounts. |
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A nurtirent material prepared for the growth of microorganisms in a laboratory. |
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they form a small intial outgrowth that enlarges until its size approaches that of the parent cell, and then seperates. |
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The time required for a cell to divide (and its population to double) is called... |
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Phases of growth : Lag Phase. |
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the number of cells changes very little becasue the cells do not immediantly reproduce in a new medium. |
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Phases of growth: Log Phase |
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The cells begin to divide and enter a period of growth or logarithmic increase. |
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Phses of growth: The Stationary Phase. |
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The growth rate slows, the number of microbial deaths balances the number of new cells and the population stabilizes. |
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The number of deaths eventually exceeds the number of new cells formed, and the population enters this phase. This phase continues until the population is dimished to a tine fraction of the number of cells in the previous phase |
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Most probable number method |
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This statistical estimating technique is based on the fact that the greater the number of bacteria in a sample, the more dilution is needed to reduce the densite to the point at which no bacteria are left to grow in the tubes in a dilution series. |
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a measured volume of a bacterial suspension is placed within a defined area on a microscope slide. |
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a practical way of monitoring bacterial growth, cloudy with cells. |
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What instrument is used to measure turbidity |
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spectrophotometer (or colorimeter) - a beam of light is transmitted through a bacterial suspension to a light sensitive detector . |
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Another indirect way to estimate bacterial numbers is to measure a populations _______ _______. This method assumes that the amount of a certain metabolic product. |
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