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Definition
time required for a population of microbial cells to double , during cell division each daughter cell receives a chromosome and sufficient
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Definition
forms ring around center of cell; related to tubulin- which is important to cell divsion protin in eukaryotes |
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Definition
ZipA: anchor that connects FtsZ ring to cytoplasmic membrane
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ZipA: anchor that connects FtsZ ring to cytoplasmic membrane |
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Definition
helps connect FTSZ ring to membrane and also recruits other divisome proteins
related to actin most abundant protein in eukaryoyotes |
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Definition
assist in determining the location of FtsZ rung |
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DNA replicates before Ftsz ring can form |
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Definition
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mind of mice work together to inhibit cell division by precenting the FtsZ ring from forming |
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Definition
sweeping minc and D aside |
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Definition
protein mediates separation of chromosomes to daughter cells |
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Definition
Major shape-determing factor in prokaryryotes
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function of MREb
2 functions
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Definition
- Forms simple cytoskeleton in cells of bacter and archeae
Forms spiral shaped bonds around the inside of the cell underneath the CM
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Term
iN WHAT KIND OF bacteria can of MREb be found?
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Definition
- Not found in COCCUS shaped bacteria only rod
- Localized synthesis of new peptidoglycan and other cell walls compounds to specific location along the cylinder of rod-shaped cell during growth
coccus + peptido
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Term
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Definition
Autolysins are a group of enzymes that exist in all bacteria containing peptidoglycan. these enzymes break down the peptidoglycan matrix in small sections so that growth and division of cells can occur. Autolysins do this by hydrolyzing the β-(1,4) bond between N-acetylmuramic acid and N-acetylglucosamine molecules. |
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Term
Peptidoglyccan synthesis
requirements |
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Definition
Preexisting peptidoglycan needs to be severed to allow newly synthesized peptidoglycan to form
autolysins
New cell wall material is added across the openings
Wall band: j |
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Term
MOST ARCHAEAL GEMONES CONTAIN WHICH PROTEINS? |
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Definition
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Term
PROCESS OF PEPTIDOGLYCAN SYNTHESIS AND CELL DIVISION |
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Definition
links in the peptidoglycan must be broken, new peptidoglycan monomers must be inserted, and the peptide cross links must be resealed.
- autolysinsp-
- Bactoprenol- l
- Glycolases- e
- Transpeptidation-
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step 1 of synthesis, what occurs and by what? |
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Definition
autolysin
- autolysinsp- break glycosidic bonds/ Break the peptide cross-bridges- FtsZ ring, small openings in the wall are created
wall band created- new and old peptidoglycan
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Definition
bactoprenol- inserts petodiglycan precurso c5 alcohol, which bonds to G/M petapeptide petido precursors |
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- Glycolases- enzymes interacting with bactoprenol- catalyze glycosidic bond formation to insert cell wall precursors into growing point of cell wall
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Term
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Definition
- Transpeptidation- final step in synthesis- FORMS PEPTIDE CROSS LINKS B/W M residues adjacent glycan chain
PEPTIDE BONDS B/W G---M(peptidoglycin bond)
3. glycosidic bond b.w (M--M bond)
penicillin inhibits m--m bond causing inhibition transpeptidase enzymes that catalyze this reaction. The activity of this enzyme is inhibited by penicillins |
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Term
how does penicillin inhibit function of transpeptidation
mechanism and consequence |
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Definition
have transpeptidase enzymes that catalyze the production of cell wall synthesis by inhibiting glycosidic bond b/w M-M. The activity of this enzyme is inhibited by penicillins causing lysing of bacteria |
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Term
summary of cell wall synthesis |
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Definition
Bactoprenol is placed into MEMBRANE, autolysin begins GM interacts with peptidoglycan with assistance of glycolases enzyme, and autolysin assist in separating membrane |
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Term
what are two criterias the generation time of bacteria are dependent on |
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Definition
1. growth medium- the amount of resources needed to grow
2. incubation conditions- temp/ heat/ ph/ enviroment
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Term
formula for expoententia growth of bacteria within x amount of hours |
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Definition
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formula for calculating generation time |
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Definition
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Term
Specific growth rate (k)
division (v)
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Definition
k = log2/g =
v= 1/g
g= t/n
N= N. * 2^n
N= final cell number
N.- intital cell number
n- number of generations during period of expoenential growth |
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read up on calculations make sure you have right formulas |
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Definition
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Term
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Definition
a closed system microbial culture of fixed volument
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Term
4 phases of typical growth curve for population of cells grown in closed systems |
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Definition
lag phase- time b/w culture is inoculated and when growth begins
expoential phase- phase are typically in healthiest state
stationary phase- growth rate of population is zero, someetimes some die while others are growing static
death phase |
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Term
stationary phase how we come to crystic growth
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Definition
some die some grow- 1. either an essential nutrient is used up or 2. waste product of org accumulates in the medium |
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Term
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Definition
rate of cell death is MUCH lower than the rate of expoential growth
this doesnt mean everything is dead |
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know what cryptic growth is |
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Definition
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Term
chemostat- growth rate is controleld by what? |
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Definition
dilution- high water outside |
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Term
growth yield is controlled by |
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Definition
concentration of limiting nutrients |
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Term
in contrast to chemostat batach culture growth conditions are constantly changing is it possibly to control batch culture growth rate and yield |
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Definition
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Term
Increasing limiting nutrient concentration results in greater mass, doe sit change growth rates |
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Definition
No, it just supples culture with necessary resourcces to continue current growth, can not "speed" up their own personal rate of growth |
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Term
when is organism "washed out" |
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Definition
high dilution rate, too much water |
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Term
cells die from saturation when |
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Definition
too low a dilution rate ( no enough water) |
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Term
what is the difference between chemostat and batch culture |
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Definition
we can c ontrol the growth of CHEMOSTAT
stat- keep track of |
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Term
what are the limitations of microscopic counts |
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Definition
- live vs dead(w/o staining)
- small cells difficult to see and can be overlooked
- precision difficult to achieve
- phase-contrast microscope required if stain is not used
- cell suspension- low density hard to count
- motile cells need to be immobilized
- debris- mistaken for cells
we already know |
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Definition
measurements of total cell numbers |
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Term
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Definition
measurement of cells or cellular particles as they move in a fluid stream past stationary set of dectators |
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Term
Calculate the cell numbers |
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Definition
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viable cell counts (plate counts) function? |
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Definition
measurement of living, reproducing population |
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Term
2 ways to perform plate counts |
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Definition
spread plate method
pour- plate method |
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Term
to obtain appropriate colon number what should be done |
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Definition
always dilute sample.
DILUTION |
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Definition
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Term
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Definition
Direct microscopic counts of natural samples typically reveal more organism then are recoverable on plates of any given culture medium. |
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why is it in great plate anomaly does Direct microscopic counts of natural samples typically reveal for more organism then are recoverable on plates of any given culture medium. |
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Definition
Microscopic methods count dead cells; whereas viable (plate counting) methods do not
Different organisms in even a very small sample may have vastly different requirements for resources and conditions in laboratory culture
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Term
function of turbidity measurements |
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Definition
indirect but: very rapid and useful method of measuring microbial growth.
(Most often measured with a spectrophotometer and measurement referred to as optical density (O.D.) |
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Term
To relate a direct cell count to a turbidity value what must be done** and why?
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Definition
A standard curve must first be established
Typically do not require destruction or significant disturbance of sample
Sometimes problematic
Microbes that forms clumps or biofilm in liquid medium |
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Definition
min/ optimum/ and maximum temperatures at which an organism grows |
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Definition
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Definition
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Definition
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Definition
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Definition
org. envolved to grow optimally under hot cold conditions |
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Definition
org with cold temp tima (most extreme representatives inhabit permanently cold enviroments) |
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Definition
org with cold temp optima (most extreme represetntatives inhabit permanently cold enviroments) |
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Term
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Definition
org grow at 0 but optima between 20-40 C, widely distrubuted in nature than pschrophiels |
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Term
prokaryotes can grow at higher temp then eukaryotes
archaea can live in hottest |
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Definition
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Term
abce 65 only what kind of life exists? |
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Definition
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Term
enzymes tht function optimally in cold have what kind of characteristics |
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Definition
- more alpha helic- flexible
- more polar less hydrophobic AA
- fewer weak bonds
- dec interaction b/w protein domains
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Term
what assists org to stay liquid in extremem colds |
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Definition
CM has high unsaturated fatty acid content, (unsaturated means more flexible) double bonds |
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Term
describe membrane of arachea |
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Definition
single layer membrane- lipid rich in saturated fatty acids which forms a strong hydrophobic enviroment then do unsaturated fatty acids |
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Term
what kind of motive force does alkaliphiles posses |
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Definition
Na motive force rather than PMF |
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Term
internal pH of cell must remail relatively neutral eventhough outside is extreme of either ends |
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Definition
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Term
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Definition
ratio of vapor pressure of air in equilibrium with a substance or solution to the vapor pressure of pure water (nothing in water except H2O) no living org in i, water activity decreases with increases of solute such as Na
think osmosis |
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Term
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Definition
glycinbetainse
prolinse
sucrose
trehalose |
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Term
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Definition
grow best at reduced water potential- have a specific requirements for NaCl |
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Definition
org that require HIGH levels of nacl for growth |
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Definition
org that live in enviroment with sugar as solute |
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Term
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Definition
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Term
mechanisms for combating low water activity (high solute concentration) in surrounding enviroment increasing internal solute concent. |
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Definition
- High solute concentration outside= water moves out to prevent this
o Pumping inorganic ions from environments into cells.
o Synthesize and accumulation of organic solutes called compatible solutes( glycin betains, preline, sucrose, trehalose):compound used by cells to counteract low water activity in surrounding environments. |
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Term
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Definition
single oxygen
superoxide
h2o2
hydroxyl radical |
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Term
how to neutralize toxic oxygen species |
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Definition
catalase
enzyme that destryo toxic oxygen spcies |
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Term
Cirtic acid cycle generates… o Alpha- ketooglutarate and oxaloacetate (OAA) Precursors of several amino acids OAA also converted to PEP; OAA PEP o Succinyl coA Required for synthesis of cytochromes and chlorophyll o Acetyl coa- nec for fatty acid biosynthesis |
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Definition
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