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chapter 5-
microbiology nutrition, culture, and metabolism
76
Microbiology
Undergraduate 3
03/09/2011

Additional Microbiology Flashcards

 


 

Cards

Term
2 forms of metabolism
Definition

catabolic reactions ( catabolism)

anabolic reactions (anabolism

Term
catabolic reaction vs anabolic reaction
Definition

Catabolic rx: chemical reactions that result in the breakdown of more complex organic molecules into simpler substances.

  -Catabolic reactions usually release energy that is used to drive chemical reactions.

 

Anabolism refers to chemical reactions in which simpler substances are combined to form more complex molecules.  Anabolic reactions usually require energy.

 

Anabolic reactions build new molecules and/or store energy.

 

The energy of catabolic reactions is used to drive anabolic reactions.

Term
missing notes for 5.1
Definition
Term
defined vs complex media
Definition
defined we know EXACTLY how much of eveything is in here, complex, chemically undefined (uknown components or unknown amounts of each)
Term
example of complex media
Definition
TSA
Term
selective vs differnetial media
Definition

   selective media - contain compounds that selectively inhibit growth of some microbes, but not others ( abx resistance is an example)

   differential media- contain an INDICATOR usually a dye that detects particular chemical reactions occurring during growth

 

Term

uniqueness of MacConkey agar

 

how does these components relate to both selective and differential medias??***

Definition

has properties of both selective and differential media

 

contains (bile, salt lactose, peptone)

bile kills gram(+), allowing G(-) to grow

 

    ecoli + fermentation --> lactic acid -->lactic acid produces a media ph<7 (acidic) causing it to turn pink

-

-salmanella does not have fermentation ability, instead it uses peptone--> Producing ammonia (pH>7)  no color change

 

 

Term

SUMMARY

 

of MacConkey agar

Definition

 

- recap- E-coli; uses fermentation of lactose producing lactic acid--> low ph -->pink

 

   

 

-recap- Salmanella--> uses peptone--> produce ammonia--> high ph --> same color

    application of medias:  KNOWWW

 

Term
pure culture vs contaiminants
Definition

pure culture- only a single kind of microbe **

 

conta

Term
tsb vs tsa
Definition
broth vs agar
Term
define sterilization**
Definition

   **- absence of ALL microorganisms including viruses (not very possible lysol)

 

Term
is it possible to get rid of 100% of bacteria
Definition
NOOO, they are everywhere
Term
Aseptic technique
Definition

 

manipulations to prevent contamination of sterile objects or microbial cultures during handling( an example is flame loop which sterilizes the environment by creating heat which causes hot air to rise removing contaminates/ clean bench is another technique/ you don't want to wear short pants and sandals should not be worn) 

 

Term
energy in bioenergetics is defined in units of
Definition
KJ measure of heat
Term
(*G) represents what?
Definition
free energy- energy release that is AVAILABLE FOR WORK
Term
what i splaced in media to make it gel
Definition
agar
Term

§ change in free energy during a reaction under standard conditions (pH 7, 25°C, 1 atm, all reactants and products at 1 M concentration) is referred to as DGo′

 

Definition
Term
-G represents
Definition

a spontaneous reaction, energy is released

 

EXERGONIC

Term
+G
Definition
ENDERGONIC
Term
Gf REPRESENTS
Definition

§ (Gfo; the energy released or required during formation of a given molecule from the elements)

 

Term
GROWTH FACTORS
Definition
organic compounds required in small amounts by certain organisms  such as vitamins AA
Term
what is the most required growth factor
Definition
vitamins and amino acids
Term

what are some of the most important vitamins

 

LOOK IN BOOK

Definition
Term
7 major atoms of life
Definition
 P S K Mg Ca Na
Term
define active site of enzyme
Definition
region where substrate binds
Term
usually enzymes are reversible but, in what cases is this not true
Definition
in hightly excess exergonic or endergonic reaction which will require another enzyme to reverse process
Term
2 small NONPROTEINS molecues that participate in catalysis but are not substrates, difference between each
Definition

prosthetic groups== bind permentantly

coenzymes- loosely bound to enzymes (vitamins)

Term
how  is E conserved in cell
Definition
oxidation reduction in the form of ATP PEP GTP (E rich compounds)
Term

oxidatized

 

reduced

Definition

loses E

 

gains e-

Term
Eo define,
Definition

difference in reduction potential between donor and acceptor, redox couples

 

farther the seperation higher the *reduction potential*

Term
define redox towe
Definition

reduced substance in redox couple, top of tower greatest tendency to accept e- (morre positive the top--> heaven)

 

Term
what is a common redox electron carrier
Definition
NAd
Term
two kinds of elecctron carriers
Definition

prosthetic

coenzyme

 

prosthetic is like a fake arm "installed"

coenzyme is cooperating but will go opposite way

 

Term
what is particularly important about NAD e- carrier
Definition
it is not comsumed in redox reaction
Term

know NAD/H cycle pg 120

 

enzyme helps nad and e- donor come together to form NADH, NADH needs enzyme and e- acceptor to return to NAD

Definition
Term
what is electron potential Eo measured in?
Definition
Volts
Term

redox tower will be on the test: will be asked which of the two reactions will be a stronger redox reaction

 

 

Definition
Term

4 E rich compounds and E stoarge

 

Definition

PEP ATP ADP AMP

coenzyme A( catly Co A)

Term
What is a source of long term E storage in prokaryotes
Definition
Glycogen/ poly- babydracytrate PHB)used to generate ATP
Term
What is a source of long term E storage in Eukaroyes
Definition
lipids/starch
Term
define fermentation
Definition

process of deriving energy from the oxidation of organic compounds, such as carbohydrates, and using an endogenous electron acceptor, which is usually an organic compound

 

ATP synthesize from E rich intermediate PEP

Term
define respiration
Definition

oxidative phospho

ATO is prod from PMF using inorganic e- donors

Term
ex of fermentation
Definition

glycolysis no e- acceptor in fermentation

(Embden meyerhof paythway)- common pathway for fermentation of glycuse --> pyruvate

Term

product of glycolysis

 

net product

Definition

gucose --> two molecules of pyruvate. + generates two molecules of adenosine triphosphate  +two molecules of NADH

 

4 total atp but 2 is used = 2 net ATP

Term
ethanol fermentation
Definition

One glucose molecule --> 2 pyruvate--> two ethanol molecules +two carbon dioxide molecules:

Term
lactate fermentation
Definition

2 atps

2 lactates

NO CO2

Term

does substrate phosphorylation use PMF?

 

Definition
not it does not use PMF
Term
oxidative phosphorylation produces how many ATP per 3 proton?
Definition
3H --> 2 atp through
Term
how many atp are produced per glycose molecule in oxidative phosphorylation
Definition
36-38 atps per glucose mole
Term
Generation of proton motive force
Definition

[image]

  1. Nadh accepts 2HE(two boys) passes to
  2. Flavored icecream (two boys
  3. flavored icecream machine gives 2e- to magical quarter, which powers the chrome car --> CHrom car C
  4. H at each of the steps are send to extracellular matric to produce PMF
Term

electron transport carriers

 

Definition
  1. NADH dehydrogenases
  2. flavoproteins
  3. FEs proteins
  4. cytochromes
  5. nonprotein: quinones
  6. fes

 

Term
products of 1 glucose in glycolysis
Definition
net: 2 atp, 2 NADH, 2 pyruvate
Term
H from PMF comes from where?
Definition

protons originate from from NADH and dissociation of water,

(-) +

Term

generation of pMF

 

(Oxidative phosphorylation requires PMF)

Definition
  1. C1: NADH donates 2he to flavored protein
  2. C2:Flavored protein donates e to succinate dehydrogenase, succinate feeds 2 H from FADH directuly to quinane pool
  3. C3: Cytochrome C bc1 donates e- from quinones to Cytochrome c, CC c shuttles transfers e to cyto a and b
  4. C5: ***Cytochrome C is an independent enzyme last in process- provides e- to o2 to produce H2O,  in TEA
  5. ATP synthase comverts PMF -->ATP
Term
How many H produces 1 atp in ATP synthase?>
Definition
3H per 1 ATP
Term
1 glucose--> 2NADH--> 9 H = 3 ATP
Definition
Term

know ORDER OF ETC

- KNOW COMPONENTS

- KNOW ENZYMES

-KNOW PRODUCTS

Definition
http://www.khanacademy.org/video/electron-transport-chain?playlist=Biology
Term

KNOW CITRIC ACID CYCLE

-NUMBER OF PRODUCTS OF EACH

- FUNCTION FOR etc

Definition
http://www.khanacademy.org/video/krebs---citric-acid-cycle?playlist=Biology
Term

KNOW GLYCOLYSIS

-FUNCTION

-ALTERNATIVE

-PRODUCTS OF EACH NET AND TOTAL

-SPECIFIC ENZYMES

Definition
http://www.khanacademy.org/video/glycolysis?playlist=Biology
Term
HOW MANY co2 and atp are produced in both glycolysis and CAC
Definition
6CO2 + 38 ATP
Term

Overall glucose tally sheet

Glycolysis

Output Total

2 ATP in 4 ATP ------> 2 ATP

2 NADH ------> 6 ATP

or ------> 4 ATP

Definition
Term

Krebs Acid Cycle output per glyccose molecule

8 NADH

2 FADH2

2 ATP ------> 2 ATP

 

1 pyruvate- 4 NAD + 1 FADH2

 

Definition
Term

Electron Transport Chain + ATP synthetase

3 X 8 NADH ------> 24 ATP

2 X 2 FADH2 -----> 4 ATP

TOTAL

36 or 38 ATPs per Glucose

Definition
Term
what two key role does Citric acid cycle play a role in
Definition

1. catabolism

2. biosynthesis (anabolism)

Term
define catabolism
Definition
et of pathways that break down molecules into smaller units and release energy.
Term
define anabolism
Definition

 

of metabolic pathways that construct molecules from smaller units.[1] These reactions require energy.

 

provides carbon skeleton for use in biosynthesis

Term
what else does citric acid cycle generate
Definition
  1. alpha- ketooglutarate
  2. oxaloacetate - precursors of several amino acids
  3. oaa also converted to PEP energy soure
  4. succinyl coA- required for synthesis of cytochromes and chlorophyll
  5. acetyl co A- needed for fatty acid biosynthesis
Term

differences between fermentation and anaerobic respiration

 

usually prokaryotic for anaerobic resp

Definition

both do not use o2,

fermentation- e producing process

anaerobic- orm of respiration using electron acceptors other than oxygen. Although oxygen is not used as the final electron acceptor, the process still uses a respiratory electron transport chain; it is respiration without oxygen. In order for the electron transport chain to function, an exogenous final electron acceptor must be present to allow electrons to pass through the system (more expensive Energetically) ferm preferred

Term

aerobic respiration and fermentation are two distinct forms of oxygen-independent energy metabolism. In anaerobic (and also aerobic) respiration, organisms channel electrons from an electron donor to a final electron acceptor through an electron transport chain, which converts the chemical energy into an electrochemical gradient. The energy stored in this gradient is then used in a second reaction by ATP synthase to generate ATP. In fermentation, ATP is directly synthesized from phosphorylated intermediates of metabolized compounds without the involvement of an electron transport chain. As there is no external electron acceptor in fermentation, cells have to produce their own electron acceptor to maintain their redox balance.

Definition
Term

chemolithotroph

 

difference b/w chemorganotroph

Definition

able to use inorganic chemicals as electron donors, usu aerobic  have ETC, and PMF

 

org- gets Carbon for biosynthesis from gluose acetate etc

litho gets it from CO2  (like autotroph)

Term
photoautotrophs use what as carbon source
Definition
CO2 for biosynthesis similar to chemolitho
Term
photoheterotroph carbon souce
Definition
use ATP to pro
Term
photophosphotylation
Definition
ligh mediated atp synthesis
Term
uniqunes of fermentation
Definition
only one that dones not phosphorlate oxy
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