Term
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Definition
catabolic reactions ( catabolism)
anabolic reactions (anabolism |
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Term
catabolic reaction vs anabolic reaction |
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Definition
Catabolic rx: chemical reactions that result in the breakdown of more complex organic molecules into simpler substances.
-Catabolic reactions usually release energy that is used to drive chemical reactions.
Anabolism refers to chemical reactions in which simpler substances are combined to form more complex molecules. Anabolic reactions usually require energy.
Anabolic reactions build new molecules and/or store energy.
The energy of catabolic reactions is used to drive anabolic reactions. |
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Definition
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Definition
defined we know EXACTLY how much of eveything is in here, complex, chemically undefined (uknown components or unknown amounts of each) |
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Definition
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selective vs differnetial media |
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Definition
• selective media - contain compounds that selectively inhibit growth of some microbes, but not others ( abx resistance is an example)
• differential media- contain an INDICATOR usually a dye that detects particular chemical reactions occurring during growth
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uniqueness of MacConkey agar
how does these components relate to both selective and differential medias??*** |
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Definition
has properties of both selective and differential media
contains (bile, salt lactose, peptone)
bile kills gram(+), allowing G(-) to grow
• ecoli + fermentation --> lactic acid -->lactic acid produces a media ph<7 (acidic) causing it to turn pink
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-salmanella does not have fermentation ability, instead it uses peptone--> Producing ammonia (pH>7) no color change
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Term
SUMMARY
of MacConkey agar |
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Definition
- recap- E-coli; uses fermentation of lactose producing lactic acid--> low ph -->pink
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-recap- Salmanella--> uses peptone--> produce ammonia--> high ph --> same color
• application of medias: KNOWWW
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pure culture vs contaiminants |
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Definition
pure culture- only a single kind of microbe **
conta |
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Definition
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Definition
• **- absence of ALL microorganisms including viruses (not very possible lysol)
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is it possible to get rid of 100% of bacteria |
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Definition
NOOO, they are everywhere |
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Term
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Definition
manipulations to prevent contamination of sterile objects or microbial cultures during handling( an example is flame loop which sterilizes the environment by creating heat which causes hot air to rise removing contaminates/ clean bench is another technique/ you don't want to wear short pants and sandals should not be worn)
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Term
energy in bioenergetics is defined in units of |
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Definition
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Term
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Definition
free energy- energy release that is AVAILABLE FOR WORK |
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Term
what i splaced in media to make it gel |
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Definition
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Term
§ change in free energy during a reaction under standard conditions (pH 7, 25°C, 1 atm, all reactants and products at 1 M concentration) is referred to as DGo′
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Definition
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Definition
a spontaneous reaction, energy is released
EXERGONIC |
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Definition
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Definition
§ (Gfo; the energy released or required during formation of a given molecule from the elements)
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Definition
organic compounds required in small amounts by certain organisms such as vitamins AA |
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Term
what is the most required growth factor |
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Definition
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Term
what are some of the most important vitamins
LOOK IN BOOK |
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Definition
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Definition
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define active site of enzyme |
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Definition
region where substrate binds |
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Term
usually enzymes are reversible but, in what cases is this not true |
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Definition
in hightly excess exergonic or endergonic reaction which will require another enzyme to reverse process |
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Term
2 small NONPROTEINS molecues that participate in catalysis but are not substrates, difference between each |
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Definition
prosthetic groups== bind permentantly
coenzymes- loosely bound to enzymes (vitamins) |
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Term
how is E conserved in cell |
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Definition
oxidation reduction in the form of ATP PEP GTP (E rich compounds) |
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Term
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Definition
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Term
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Definition
difference in reduction potential between donor and acceptor, redox couples
farther the seperation higher the *reduction potential* |
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Term
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Definition
reduced substance in redox couple, top of tower greatest tendency to accept e- (morre positive the top--> heaven)
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Term
what is a common redox electron carrier |
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Definition
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Term
two kinds of elecctron carriers |
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Definition
prosthetic
coenzyme
prosthetic is like a fake arm "installed"
coenzyme is cooperating but will go opposite way
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Term
what is particularly important about NAD e- carrier |
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Definition
it is not comsumed in redox reaction |
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Term
know NAD/H cycle pg 120
enzyme helps nad and e- donor come together to form NADH, NADH needs enzyme and e- acceptor to return to NAD |
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Definition
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what is electron potential Eo measured in? |
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Definition
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redox tower will be on the test: will be asked which of the two reactions will be a stronger redox reaction
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Definition
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Term
4 E rich compounds and E stoarge
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Definition
PEP ATP ADP AMP
coenzyme A( catly Co A) |
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Term
What is a source of long term E storage in prokaryotes |
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Definition
Glycogen/ poly- babydracytrate PHB)used to generate ATP |
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Term
What is a source of long term E storage in Eukaroyes |
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Definition
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Term
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Definition
process of deriving energy from the oxidation of organic compounds, such as carbohydrates, and using an endogenous electron acceptor, which is usually an organic compound
ATP synthesize from E rich intermediate PEP |
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Term
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Definition
oxidative phospho
ATO is prod from PMF using inorganic e- donors |
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Term
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Definition
glycolysis no e- acceptor in fermentation
(Embden meyerhof paythway)- common pathway for fermentation of glycuse --> pyruvate |
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Term
product of glycolysis
net product |
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Definition
gucose --> two molecules of pyruvate. + generates two molecules of adenosine triphosphate +two molecules of NADH
4 total atp but 2 is used = 2 net ATP |
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Definition
One glucose molecule --> 2 pyruvate--> two ethanol molecules +two carbon dioxide molecules:
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Term
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Definition
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Term
does substrate phosphorylation use PMF?
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Definition
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Term
oxidative phosphorylation produces how many ATP per 3 proton? |
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Definition
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Term
how many atp are produced per glycose molecule in oxidative phosphorylation |
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Definition
36-38 atps per glucose mole |
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Term
Generation of proton motive force |
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Definition
[image]
- Nadh accepts 2HE(two boys) passes to
- Flavored icecream (two boys
- flavored icecream machine gives 2e- to magical quarter, which powers the chrome car --> CHrom car C
- H at each of the steps are send to extracellular matric to produce PMF
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Term
electron transport carriers
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Definition
- NADH dehydrogenases
- flavoproteins
- FEs proteins
- cytochromes
- nonprotein: quinones
- fes
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Term
products of 1 glucose in glycolysis |
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Definition
net: 2 atp, 2 NADH, 2 pyruvate |
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Term
H from PMF comes from where? |
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Definition
protons originate from from NADH and dissociation of water,
(-) + |
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Term
generation of pMF
(Oxidative phosphorylation requires PMF) |
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Definition
- C1: NADH donates 2he to flavored protein
- C2:Flavored protein donates e to succinate dehydrogenase, succinate feeds 2 H from FADH directuly to quinane pool
- C3: Cytochrome C bc1 donates e- from quinones to Cytochrome c, CC c shuttles transfers e to cyto a and b
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C5: ***Cytochrome C is an independent enzyme last in process- provides e- to o2 to produce H2O, in TEA
- ATP synthase comverts PMF -->ATP
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Term
How many H produces 1 atp in ATP synthase?> |
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Definition
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Term
1 glucose--> 2NADH--> 9 H = 3 ATP |
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Definition
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Term
know ORDER OF ETC
- KNOW COMPONENTS
- KNOW ENZYMES
-KNOW PRODUCTS |
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Definition
http://www.khanacademy.org/video/electron-transport-chain?playlist=Biology |
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Term
KNOW CITRIC ACID CYCLE
-NUMBER OF PRODUCTS OF EACH
- FUNCTION FOR etc |
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Definition
http://www.khanacademy.org/video/krebs---citric-acid-cycle?playlist=Biology |
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Term
KNOW GLYCOLYSIS
-FUNCTION
-ALTERNATIVE
-PRODUCTS OF EACH NET AND TOTAL
-SPECIFIC ENZYMES |
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Definition
http://www.khanacademy.org/video/glycolysis?playlist=Biology |
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Term
HOW MANY co2 and atp are produced in both glycolysis and CAC |
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Definition
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Term
Overall glucose tally sheet
Glycolysis
Output Total
2 ATP in 4 ATP ------> 2 ATP
2 NADH ------> 6 ATP
or ------> 4 ATP
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Definition
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Term
Krebs Acid Cycle output per glyccose molecule
8 NADH
2 FADH2
2 ATP ------> 2 ATP
1 pyruvate- 4 NAD + 1 FADH2
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Definition
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Term
Electron Transport Chain + ATP synthetase
3 X 8 NADH ------> 24 ATP
2 X 2 FADH2 -----> 4 ATP
TOTAL
36 or 38 ATPs per Glucose
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Definition
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Term
what two key role does Citric acid cycle play a role in |
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Definition
1. catabolism
2. biosynthesis (anabolism) |
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Term
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Definition
et of pathways that break down molecules into smaller units and release energy. |
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Term
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Definition
of metabolic pathways that construct molecules from smaller units.[1] These reactions require energy.
provides carbon skeleton for use in biosynthesis |
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Term
what else does citric acid cycle generate |
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Definition
- alpha- ketooglutarate
- oxaloacetate - precursors of several amino acids
- oaa also converted to PEP energy soure
- succinyl coA- required for synthesis of cytochromes and chlorophyll
- acetyl co A- needed for fatty acid biosynthesis
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Term
differences between fermentation and anaerobic respiration
usually prokaryotic for anaerobic resp |
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Definition
both do not use o2,
fermentation- e producing process
anaerobic- orm of respiration using electron acceptors other than oxygen. Although oxygen is not used as the final electron acceptor, the process still uses a respiratory electron transport chain; it is respiration without oxygen. In order for the electron transport chain to function, an exogenous final electron acceptor must be present to allow electrons to pass through the system (more expensive Energetically) ferm preferred |
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Term
aerobic respiration and fermentation are two distinct forms of oxygen-independent energy metabolism. In anaerobic (and also aerobic) respiration, organisms channel electrons from an electron donor to a final electron acceptor through an electron transport chain, which converts the chemical energy into an electrochemical gradient. The energy stored in this gradient is then used in a second reaction by ATP synthase to generate ATP. In fermentation, ATP is directly synthesized from phosphorylated intermediates of metabolized compounds without the involvement of an electron transport chain. As there is no external electron acceptor in fermentation, cells have to produce their own electron acceptor to maintain their redox balance. |
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Definition
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Term
chemolithotroph
difference b/w chemorganotroph |
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Definition
able to use inorganic chemicals as electron donors, usu aerobic have ETC, and PMF
org- gets Carbon for biosynthesis from gluose acetate etc
litho gets it from CO2 (like autotroph) |
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Term
photoautotrophs use what as carbon source |
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Definition
CO2 for biosynthesis similar to chemolitho |
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Term
photoheterotroph carbon souce |
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Definition
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Term
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Definition
ligh mediated atp synthesis |
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Definition
only one that dones not phosphorlate oxy |
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