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Chapter 5: Research Methods
Chapter 5: Research Methods
25
Physiology
Undergraduate 4
10/23/2011

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Term
What are the 3 types of brain lesions?
Definition
Ablation (removal), electrical current, chemical lesion

-1st two destroy cell bodies & axons
-3rd destroys only cell bodies
Term
Excitotoxic lesion
Definition
Chemical lesion produced by the injection of an excitatory amino acid
Term
Sham lesion
Definition
A placebo procedure that duplicates all the steps of a lesion, except for the one that actually causes brain damage
Term
Stereotaxic apparatus
Definition
Device that puts an electrode or cannula into the brain
Term
Anterograde tracer
Definition
Taken up by dendrites/cell bodies -> axon terminals (outputs)
Term
Retrograde tracer
Definition
Taken up by axon terminals -> cell bodies
Term
Antibodies
Definition
Proteins that bind to their target (antigen) w/ great specificity
Term
What can we use immunocytochemistry for?
Definition
To identify/localize NT, enzymes, & receptors/transporters
Term
Computerized tomography (CT)
Definition
2D picture of a slice through the body
Term
Magnetic resonance imaging (MRI)
Definition
3D- radio waves/strong magnetic field
Term
Microelectrode
Definition
Records activity of a single neuron
Term
Single-unit recording
Definition
The recording of the electrical activity of a single neuron
Term
Macroelectrode
Definition
Records the activity of large numbers of neurons in a particular region of the brain
Term
Electroencephalogram (EEG)
Definition
Measures large scale brain activity-millions of neurons
Term
2-deoxyglucose (2-DG)
Definition
A radioactive sugar that enters cells, but is not metabolized
Term
Autoradiography
Definition
Procedure that locates radioactive substances in a slice of tissue
Term
PET scan
Definition
Measures activity, not just structure
Term
Fos
Definition
A protein produced in the nucleus of a neuron in response to stimulation of a synapse
Term
Microdialysis
Definition
Procedure for analyzing chemicals in the extracellular fluid
Term
Cannula
Definition
Tube used to inject small amounts of a drug into the brain
Term
What are the four types of experiments?
Definition
Necessity
Block
Mimic
Measure
Term
Necessity
Definition
Remove A, then no B or C
Term
Block
Definition
Blocking B should block C
Term
Mimic
Definition
Activating B should cause C
Term
Measure
Definition
A makes B happen
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