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Chapter 4
Prokaryotes
48
Microbiology
Undergraduate 1
07/17/2011

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Cards

Term
How are prokayotes different from eukaryotes (3)?
Definition
1. no nucleus
2. cell wall makeup
3. no membrane-bound organelles
Term
Which structures are common to ALL bacteria? (4)
Definition
  1. cell membrane
  2. cytoplasm
  3. ribosomes
  4. chromosomes (usually 1)
Term
Which structures are found in MOST bacteria? (2)
Definition
  1. cell wall
  2. glycocalyx
Term
Which structures are found in SOME bacteria?
Definition
  1. flagella
  2. pili
  3. fimbrae
  4. capsules
  5. slime layers
  6. inclusions
  7. actin cytoskeleton
  8. endospores
Term
What are the three parts of a flagellum?
Definition

1. filament (long part)

2. hook

3. Basal body (anchors to cell)

Term
What are the four types of arrangements of flagella?
Definition
  1. Monotrichous (1 flagellum)
  2. Amphitrichous (two flagella, one on each end of cell)
  3. Lophotrichous (Many flagella on ONE END of cell)
  4. Peritichous (Many falgella all over cell)
Term
What is chemotaxis and what are two types of it?
Definition

flagellated bacteria move in response to chemical signals

 

  • positive chemotaxis- toward direction of favorable stimulus (nutrients)
  • negative chemotaxis- away from repellent compound (harmful)
Term
What is phototaxis?
Definition
Flagellated movement in response to light
Term
How do flagella move?
Definition

Runs and tumbles

 

Runs are longer going up gradient

Tumbles to test environment

Term
What are cork-screwed bacteria called?
Definition
Spirochetes
Term
What type of flagella do spirochetes have, where are they located, and what kind of movement do they cause?
Definition
  • axial filaments aka periplasmic flagella
  • enclosed in space between the cell wall and the cell membrane
  • give corkscrew or wriggly motion
Term
What are pili and what's their function?
Definition
  • elongated, rigid tubular structures made of the protein pilin
  • used in conjugation- partial transfer of DNA from one cell to another
Term

Pili are found on in gram-positive bacteria

True or False

Definition
False- only found in gram-negative
Term
What are Fimbriae? What's their function?
Definition

Fimbriae are small, bristlelike fibers found on the surface of a bacterial cell and most of them contain protein.

 

They stick to each other and to surfaces, causing colonization

Term
Where is the glycocalyx located and what is it made of?
Definition

Outside the cell envelope

 

Made of repeating polysaccharide units, protein, or both

Term
What are the functions of the glycocalyx (3)?
Definition

Protects the cell

 

Helps the cell adhere to the environment

 

Protects the cell against phagocytes (biofilm)

Term
When is a glycocalyx termed a slime layer? What does it do?
Definition

1. When the glycocalyx is loosely shielding the bacteria

 

2. It protects the bacteria from loss of water and nutrients; also adherance

Term
When is a glycocalyx termed a capsule? What's its function?
Definition

1. When the glycocalyx is bound more tightly to the cell and is denser and thicker

 

2. Protects from phagocytosis

Term
What are the differences between gram + and gram - cell envelope structures?
Definition
  • Gram + has two layers: cell wall (thick) and cytoplasmic membrane--- also contains teichoic acid and lipoteichoic acid
  • Gram - has three layers: outer membrane, cell wall (thin), and cytoplasmic membrane--- also has perplasmic space surrounding the cell wall
Term

What is the cell wall made of?

What does it keep the cell from doing?

What medical technology targets the cell wall?

Definition

peptidoglycan

 

keeps cell from rupturing because of changes in pressure due to osmosis

 

Antibiotics target the cell wall, giving it little protection from lysis

Term
What are the two layers of the gram - outer membrane?
Definition

Uppermost layer contains lipopolysaccharides (LPS)

 

Innermost layer made of phospholipid layer-- anchored to cell wall

Term
What 3 types of bacteria have nontypical cell walls?
Definition
  1. Mycobacterium (have mycolic acids- fatty acid chain giving high degree of resistance; basis for acid-fast staining)
  2. Archae (unusual and chemically distinct)
  3. Mycoplasmas (no cell wall)
Term
Describe a mycoplasma and its cell membrane.
Definition

Mycoplasmas are very small and range in shape and can be found in many habitats.

 

Its cell membrane is stabilized by sterols and is resistant to lysis.

 

*Important medical species: Mycoplasma pneumonia (aka walking pneumonia)

Term
What are L-forms and specific names for them?
Definition

L-form arise naturally from a mutation in the wall-forming genes. They have no distinct shape.

 

Gram + are called protoplast

Gram - are called spheroplast

Term
What's a bacterial cell membrane (cytoplasmic membrane) made of and what are its functions (3)?
Definition
  • Contains primarily phospholipids and proteins

Provides site for functions such as energy reactions, nutrient processing, and synthesis

 

Regulates transport

 

Secretion

Term

What is a bacterial chromosome?

Where is it located?

What's its method of replication called?

Definition
  • single circular strand of DNA
  • aggregated in a dense area called the nucleoid
  • replicates through binary fission (more rapid than human DNA because happens all at once)
Term
Describe Plasmids.
Definition
  • Nonessential pieces of DNA forming in double-stranded circles
  • Have protective traits (drug resistance or production of toxins)
  • Can pass between bacteria via pili or phages
Term
What is the S count for prokaryotic ribosomes?
Definition

70S

 

 

Humans have 80S

Term
What's the function of inclusion bodies?
Definition

Serve as storehouse of nutrients

(bacteria lay down nutrients in them during periods of nutrient abundance; use when nutrients are depleted)

Term
What does the actin cytoskeleton do? Where is it located?
Definition

Contributes to cell shape

 

Arranged in helical ribbons around cell just under cell membrane

Term
What are the two phases of a bacteria's life cycle?
Definition
  1. vegatative (metabolically active and growing)
  2. endospore (metabolically inactive)
Term
What are the only genus known to produce endospores (3)? Are they gram + or gram -?
Definition

Bacillus

Clostridium

Sporosarcina

 

All Gram +

Term
What are the three general shapes of bacteria?
Definition
  1. coccus (spherical)
  2. Bacillus (rod)
  3. Spirillum (spiral)

Coccobacillus= short and plump

Vibrio= gently curved bacillus

Term
What's pleomorphism?
Definition
when cells of a single species cary to some extent in shape and size
Term
What is a sarcina?
Definition
cubical packet of cocci
Term
What are palisades?
Definition
row of bacilli cells oriented side by side
Term
What was the early classification system of prokaryotes?
Definition
the phenotypic traits (shape, arrangement, growth, habitat, color, etc)
Term
What is the current classification system of prokayotes?
Definition
phenotypic information with rRNA sequencing
Term
How do we define a bacterial species?
Definition
A collection of bacterial cells all of which share an overall similar pattern of traits
Term
What is a bacterial subspecies/strain/type?
Definition
Bacteria of the same species that have differing characteristics (ex. extra plasmids)
Term
What's a serotype?
Definition
Representatives of a species that stimulate a distinct pattern of antibody (serum) responses in their hosts, because of distinct surface molecules
Term
What are examples of obligate intracellular parasites (2)?
Definition
Rickettsias and Chlamydias
Term
Describe Rickettsias and their characteristics.
Definition

Gram-negative

 

Alternate between mammals and blood-sucking anthropods

 

Cannot survive or multiply outside host cell

 

Cannot carry out metabolism completely on their own

 

Examples: Rocky Mountain Spotted Fever (Rickettsia rickettsii) and endemic typhus (Rickettsia typhi)

Term
Describe Chlamydias and how they're different from Rickettsias.
Definition

require host cells for growth and metabolism

 

Not closely related

 

Not transmitted by arthropods

 

Examples: Chlamydia trachomatis (STD) and Chlamydophilia pneumonia (lung infections)

Term
What's distinct about photosynthetic bacteria?
Definition

Some produce oxygen during photosynthesis

 

Give us most of the oxygen we have today

Term
What are cyanobacteria also known as? Why are they distinct?
Definition

AKA Blue-Green Bacteria

 

Contain chlorophyll and have gas filled inclusions which allow them to float on water

 

Term

What are Green and Purple Sulfur Bacteria?

 Where do they live?

Definition

Photosynthetic but don't give off oxygen

 

Live in sulfur springs, freshwater lakes, and swamps

Term

What makes Archaea distinct?

What types of environments can they live in?

Definition

Type of prokaryotes that have been around the longest-- they have adapted to every environment

 

They love extreme environments including extreme heat/cold, salt, acid, pH, pressure, and atmospheres

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