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Chapter 4
Functional Anatomy of Prokaryotic and Eukaryotic Cells
71
Microbiology
Undergraduate 2
04/17/2011

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Term
Bacteria used as insecticide
Definition
Bacillus thuringensis
Term
Bacteria used in warfare causing 1. anthrax and 2. food poisening
Definition
Bacillus anthracis and Clostridium botulinum
Term
5 features of prokaryotes
Definition
1. one circular chromosome
2. no histones
3. no organelles
4. peptidoglycan cell walls
5. binary fission
Term
Bacillus
Definition
rod shaped
Term
coccus
Definition
spherical
Term
spirillum
Definition
wave shaped
Term
vibrio
Definition
curved rod
Term
spirochete
Definition
corkscrew
Term
strepto
Definition
chain
Term
staphyl
Definition
cluster
Term
8 cell
Definition
sarcinae
Term
2 examples of pleomorphic bacteria
Definition
1. Rhizobium
2. Corynebacterium
Term
adavtages of having small size (3)
Definition
1. accumulate nutrients quickly
2. metabolize quickly
3. grow quickly
Term
largest bacteria known
Definition
Epulopiscium sp.
Term
What is glycocalyx made of?
Definition
polysaccarhides
Term
2 functions of glycocalyx
Definition
1. source of food under adverse conditions
2. absorbs water, prevents dehydration
Term
biofilm is associated with this type of glycocalyx
Definition
slime layer
Term
why does a capsule increase pathogencity of bactuera?
Definition
white blood cells cannot grab them, prevents phagocytosis
Term
3 functions of cell wall
Definition
rigidity, shape, prevents osmotic lysis
Term
Tetrapeptide side chaings hang from...
Definition
NAM
Term
Peptide cross bridges do what
Definition
link layers of PG together
maintain integrety of cell wall
Term
# layers of PG in Gram + cell wall
Definition
10-12
Term
Teichoic acids roles and composition
Definition
present in gram +
regulate movement of cations

alcohol/glycerol and phosphate
provide antigenic variation
Term
antigentic variation
Definition
ability of distinguish between bacterial cells
Term
Gram negative cell wall
Definition
thin layer of PG
no teichoic acids
outer membrane
Term
composition of outer membrane in gram neg
Definition
LPS (lipopolysarcharide)
-made of Lipid A and O polysaccharide
Term
Lipid A
O polysaccharide
Definition
endotoxin
antigen, used for distinguishing bacteria
Term
example of a bacteria whose antigen identifies it
Definition
E Coli O157:H7
Term
how penecillan works
Definition
kills PG in gram + bacteria, specifically by breaking peptide cross bridges
Term
acid fast cell wall composition
Definition
waxy lipid (mycolic acid) bound to peptidoglycan
Term
2 examples of acid fast cell wall bacteria
Definition
1. Mycobacterium - causes TB
2. Nocardia
Term
mycoplasma bacteria
Definition
-lack cell walls
-there are sterols in plasma membrane help protect from lysis
-smallest of all bacteria, most contaminating in labs
Term
archea cell wall composition
Definition
pseudomurein or pseudoPG
lacks NAM and D-amino acids
Term
Plasma membrane composition
Definition
phopholipid bilayer and proteins
Term
fluid mosaic model
Definition
lipids and proteins are free to move around, about as viscous as olive oil
Term
functions of plasma membrane
Definition
-selective permiability
-enzymes for ATP production (lack mitochondria)
-photosynthetic pigments
Term
damage to plasma membrane caused by
Definition
1. alcohol
2. detergent
3. polymyxin antibiotics
casue leakage,death
Term
simple diffusion
Definition
passive transport, usually gaseous molecules
Term
facilitated diffusion
Definition
passive, larger molecules ex. glycerol
goes through transporter protein
Term
osmolerity in cell
Definition
0.85%, ex. saline
Term
classical active transport
Definition
-uses ATP or PMF
-substance not altered across membrane
-uses transporter proteins
Term
group translocation
Definition
molecular is chemically altered, this prevents it from leaving cell
ex. glucose is phosphorolated
Term
what bacteria does not have flagella?
Definition
cocci
Term
flagellin proteins in the filament are
Definition
H proteins, recognized by immune system
ex. E Coli
Term
monotrichous
Definition
1 flagellum at 1 end
Term
ampthitrichous
Definition
1 flagellum at each end
Term
lophotrichous
Definition
many flagellum at one end
Term
peritrichous
Definition
entire cell wall has flagella
Term
chemotaxis and phototaxis
Definition
movement towards or away from a chemical or light
Term
what bacteria causes stomach ulcers
Definition
Helicobacter pylaorii
strong flagella can swim through mucous
Term
what bacteria causes stomach ulcers?
Definition
Helicobacter pylaorii
strong flagella can swim through mucous
Term
mode of movement by bacteria in ocean
Definition
gaseous vesicles
Term
what type of cells have pili and fimbriae?
Definition
gram negative
Term
function of pili
Definition
gliding/twitching movement
DNA transfer
Term
Fimbriae
Definition
used to attach to things, eg inside of small intestine
Term
structures found in cytoplasm
Definition
DNA and ribosomes
Term
bacterial chromosome (nucleoid) characteristics (4)
Definition
1. no nuclear membrane
2. no histones
3. circular DNA
4. Haploid DNA (only 1 copy of each gene)
Term
plasmids description
Definition
circular, double stranded DNA, extra-chromosomal
autonomis from nucleoid
not required by cell
Term
benefits of plasmids
Definition
can contain genes for:
-antibiotic resistance
-toxic metal resistance
-production of enzymes
Term
transfer of plasmids
Definition
takes place through pili
Term
function of ribosomes
Definition
make proteins
Term
somposition of bacterial ribosome
Definition
small subunit = 30S
large subunit = 50S
complete ribosome = 70S
Term
human ribosome complete unit
Definition
80S
Term
antibiotic effect on ribosomes
Definition
antibiotics can be targeted towards bacterial ribosomes without harming human ribosomes
Term
content and use of metachromatic granule
Definition
phosphate, used to make ATP
Term
presence of metachromatic granule indicates what species of bacterium?
Definition
Corynebacterium diphtheriae
Term
who produces endospores?
Definition
gram +
Term
when are endospores produced?
Definition
when a bacterium is stressed because of lack of C or N
Term
how long can endospores survive?
Definition
100k+ years
Term
the process of endospore formation is known as
Definition
sporulation
Term
endospores are made of what?
Definition
DPA dipicolnic acid
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