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Know EXACT contents of media |
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Exact contents are not known. Ex. V8 juice |
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Liquid Media, used to grow large colonies of Microbes |
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Gelatin type media called Agar is used. Made to grow discrete colonies on it's surface |
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A small amount of Agar is used. Used to see if microbes are motile |
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Exactly that. A general purpose media that grows most microbes |
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Has ingredients that will allow some microbes to grow while others not to grow |
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Allows you to tell one microbe from another |
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Addint additional nutrients for microbes that won't grow ona General Media |
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Several Microbes ina sample |
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Microbes introduced that shouldn't be present. |
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Describe the streak plate method |
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used to isolate microbes into pure culture by spreading teh microbe across a Petri dish in three or four overlapping areas |
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Describe Serial Dilution Technique |
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Definition
Take a known volume of inoculum from one test tube to the next and then spreading known volumes from each tube onto Petri dishes |
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Streak plate is used to do what? |
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Definition
cut down the total amounts of all Microbes |
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Serial Dilution is used for what? |
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Definition
Cut down the number of diffrent microbes |
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Nutrient Agar is what type of media?? |
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Green Algae Media is what type of media? |
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mannitol Salt Agar is what? |
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Differential and Selective |
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Eosin Methylene Blue medium |
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Best used for slides that have been stained |
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Best used for living cells |
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Best when looking at living cells |
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Uses a diffrent light and the object can be injected with dyes that attach to diffrent things like protiens or starches |
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Compound light microscopes |
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Uses light to highlight or silouette the objects |
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Fluorescent light microscopes |
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Similar to compound light microscopes but uses a diffrent light that can pick up diffrent dyes that are inserted |
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Will show much smaller objects than the compound light |
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Scanning electron microscope |
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Transmission electron microscope |
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Stabilizing the internal structure of the cell |
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Cut into small sections or smeared |
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Everything dyed the same color |
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Stains the background and not the object |
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Will stain diffrent cellular structures |
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What cellular structures do you see with a light microscope? |
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Whole cells, large organelles, and bacteria |
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What additional structures can you see with an electron microscope? |
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Phospholipid bilayer, all organelles, ribosomes, viruses, and DNA |
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Stains used in Gram Stain?? |
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Definition
Chrystal Blue and Safranin |
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