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Chapter 22 - Introduction to Analytical Separations
E.O.C. Vocabulary of Chapter 22 from "Quantitative Chemical Analysis" by Daniel C. Harris
17
Chemistry
Undergraduate 3
11/06/2012

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Term
Adjusted Retention Times
Definition
In chromatography, this parameter is the time needed for mobile phase to travel the length of the column.
Term
Adsorption Chromatography
Definition
A technique in which solute equilibrates between the mobile phase and adsorption sites on the stationary phase.
Term
Affinity Chromatography
Definition
A technique in which a particular solute is retained by a column by virtue of a specific interaction with a molecule covalently bound to the stationary phase.
Term
Chromatogram
Definition
A graph showing chromatography detector response as a function of elution time or volume.
Term
Diffusion
Definition

 

Net transport of a solute from a region of high concentration to a region of low concentration caused by the random movement of molecules in a liquid or gas (or, very slowly, in a solid).

Term
Diffusion Coefficient
Definition
Defined by Fick's first law of diffusion: J = -D(dc/dx), where J is the rate at which molecules diffuse across a plane of unit area and dc/dx is the concentration gradient in the direction of diffusion.
Term
Distribution Coefficient
Definition

For a solute partitioned between two phases, the total concentration of all forms of solute in phase 2 divided by the total concentration in phase 1.

 

Term
Eluate
Definition
What comes out of a chromatography column. Also called effluent.
Term
Eluent
Definition
Solvent applied to the beginning of a chromatography column.
Term
Elution
Definition
Process of passing a liquid or a gas through a chromatography column.
Term
Extraction
Definition
Process in which a solute is transferred from one phase to another.
Term
Ion-Exchange Chromatography
Definition
A technique in which electrolytes are separated from nonelectrolytes by an ion-exchange resin.
Term
Linear Flow Rate
Definition
In chromatography, the distance per unit time traveled by the mobile phase.
Term
Longitudinal Diffusion
Definition
Diffusion of solute molecules parallel to the direction of travel through a chromatography column.
Term
Miscible Liquids
Definition
Two liquids that form a single phase when mixed in any ratio.
Term
Mobile Phase
Definition
In chromatography, the phase that travels through the column.
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