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Chapter 21
Prokaryotes and Viruses
127
Biology
Undergraduate 3
04/09/2006

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Term
microorganisms
Definition
prokaryotic cells and single-celled protistans too small to be seen without the aid of a microscope.
Term
What is the kingdom that contains the most individuals?
Definition
prokaryotic
Term
Pathogens
Definition
infectious, diease causing agents that invade target organisms and multiply inside or on them.
Term
Where do prokaryotic cells show the greatest diversity?
Definition
in their means for securing resources
Term
photoautotrophic species
Definition
build organic compounds by photosynthesis, light driven self-feeders.
Term
What is the energy and carbon source for photoautotrophic species?
Definition
energy source is the sun and carbon source is Carbon dioxide,
Term
What are the two places photoautotrophs may get their electrons and hydrogen?
Definition
some get it from water molecules and release oxygen as a waste product. Others get them from inorganic compunds such as gaseous hydrogen and hydrogen sulfide.
Term
chemoautotrophs
Definition
self-feeders that use carbon dioxide as their carbon source.
Term
Two different ways chemoautotrophs obtain required energy?
Definition
Some oxidize organic compounds for energym others oxidize inorganic compunds such as iron, hydrogen, sulfur and nitrogen compounds.
Term
Photoheterotrophs
Definition
not self-feeders. They capture sunlight energy for photosynthesis. They get carbon from various organic compounds such as fatty acids and carbohydrates that other organisms produce.
Term
Chemoheterotrophic prokaryotc cells
Definition
parasites or saprobes, not self-feeders.
Term
what is the difference between a saprobe and a parasite?
Definition
a parasite draws glucose and other nutrients from a living host, where saprobes get nutrients by digesting organic products, wastes, or remains of other organisms.
Term
the three shapes of bacteria
Definition
coccus, bacillus, spirillum
Term
coccus
Definition
sphere
Term
bacillus
Definition
rod-shaped
Term
spirillum
Definition
spiral shaped
Term
Prokaryotic
Definition
means these cells were around before the evolution of the nucleated cell.
Term
What is usually around the plasma membrane in prokaryotic cells?
Definition
the cell wall
Term
what is the purpose of the cell wall?
Definition
This semi-rigid, permeable structure helps the cell maintain shape and resist rupturing when internal fluid pressures rise.
Term
eubacterial cell walls are composed of...
Definition
peptidoglycan
Term
describe the structure of the peptiglycans:
Definition
in these molecules, peptide groups crosslink many polysaccride strands to one another.
Term
Gram stain
Definition
a sample of unknown species is exposed to purple dye, then iodine, then an alcohol wash, then a counter stain.
Term
what color represents Gram-positive?
Definition
purple
Term
what color represents Gram-negative?
Definition
pink. Loses the purple during the wash, but the counter stain stains it pink.
Term
glycocalyx
Definition
a sticky mesh that often encloses the cell wall.
Term
what is the glycocalyx composed of?
Definition
polysaccrides, polypeptides, or both.
Term
what does the glycocalyx look like when orgainized? Not organized?
Definition
when organized, it forms a capsule. When not organized, it forms a slime layer.
Term
what is the purpose of the glycocalyx?
Definition
it helps a prokaryotic cell attach to teeth, mucous membranes, and other surfaces. Its also helps the cell avoid being phagocized by other cells that are usually fighting infection in their host cell.
Term
bacterial flagella
Definition
some species have one or more of these motile structures. They rotate like a propeller.
Term
pili
Definition
some species also have these short, filamentous proteins that project above the cell wall.
Term
pupose of the pili?
Definition
it helps the cells connect to each other for conjugation.
Term
How do you measure the growth of prokaryotic cells?
Definition
by increases of number of cells in a population.
Term
what is different about the K12 strain of E. coli?
Definition
it was the strain that was originally isolated from the human gut. It has been culticvated in the lab for so long that it is no longer able to grow when introduced into its natural habitat.
Term
bacterial chromosome
Definition
a circulized, double stranded DNA molecule that has only a few proteins attached to it.
Term
prokaryotic fission
Definition
the most common form of reproduction used by prokaryotic cells.
Term
the steps of prokaryotic fission:
Definition
1) The bacterial chromosome is attached to the plasma membrane before DNA replication starts. 2) Replication starts and proceeds in both directions. 3) The DNA copy is attached at a membrane site of the parent DNA molecule. 4) the two DNA molecules are moved apart by membrane growth. 5) the two seperate cells now seperate from each other.
Term
plasmid
Definition
a small, self-replicating circle of extra DNA with a few genes.
Term
F plasmid
Definition
fertility plasmid. includes genes that confer the means to engage in a form of conjugation.
Term
Describe how conjugation works:
Definition
the F plasmid carries genetic information for synthesizing a structure called a sex pilus. The sex pili at the surface of the donor cell can hook to a recipient cell and pull it right up next to the donor. a conjugation tube forms between the two cells through which a plasmid DNA is transferred.
Term
numerical taxonomy
Definition
the traits of the unidentified cell are compared with those of a known prokaryotic group.
Term
the types of traits looked at during numerical taxonomy:
Definition
cell shape, motility, staining attributes of the cell wall, nutritional requirements, metabolic patterns and the presence or absence of endospores.
Term
Another way to identify the relationship between two prokaryotic cells?
Definition
nucleic acid hybridization between the rRNA.
Term
eubacteria
Definition
the most common prokaryotic cells (typical)
Term
strain
Definition
if two cells under study show only minor differences, one of them might be classified as a strain.
Term
Three major groups of archeabacteria:
Definition
extreme thermophiles, methanogensm and extreem halophiles.
Term
extreme thermophiles
Definition
heat lovers
Term
where might you find extreme thermophiles?
Definition
geothermally heated soils, hot springs, wastes from coal mines, etc.
Term
what do extreme thermophiles use as their electron acceptor or donor?
Definition
sulfur. (strict anaerobes)
Term
solfolobus
Definition
the first extreme thermophile discovered. grows in acidic hot springs.
Term
Thermus aquaticus
Definition
an extreme thermophile that biotechnologist utilize as a source of extremely heat-stable DNA polymerases.
Term
methanogens
Definition
(methane makers) live in oxygen free habitats, such as seamps, the guts of termites and mammals and stockyards. They are strict anaerobes and oxygen kills them.
Term
how do methanogens produce ATP?
Definition
by anaerobic ellctron transfer,
Term
where do methanogens get their energy?
Definition
They get electrons from hydrogen gas or from ethanol. they use carbon dioxide as their carbon source and as their final electron acceptor.
Term
what do methanogens produce?
Definition
methane
Term
How much methane do methanogens produce?
Definition
2 billion tons per year.
Term
how might methane from methanogens drastically alter the global climate in the future?
Definition
If ocean circulation patterns change the huge methane deposits in the sea floor may be released all at once.
Term
extreme halophiles
Definition
salt lovers
Term
How do extreme halophiles produce ATP?
Definition
Most through aerobic pathways. When oxygen levels are low, they switch to photosynthesis.
Term
What unique, light-absorbing pigment do extreme halophiles have under their plasma membrane?
Definition
bacteriorholdopsin
Term
what does the absorbtion of light energy do for the extreme halophiles?
Definition
leads to an increase in H+ gradients across their plasma membrane. ATP forms when these ions follow their gradient and flow through the interior of transporter proteins, which span the membrane.
Term
cyanobacteria
Definition
once called blue-green algae, they are the classic example of photoautotrophic bacteria. Aerobic cells that engage in photosynthesis.
Term
Anabaena
Definition
Photoautotrophic bacteria. Convert nitrogen gas to ammonia for use in biosynthesis.
Term
When are heterocysts formed?
Definition
as nitrogen compounds dwindle, some of the photoautotrophic cells turn into these.
Term
what are heterocysts?
Definition
Cells that make a nitrogen-fixing enzyme. They synthesize and share nitrogen compounds with photosynthetic cells and in return they recieve carbohydrates.
Term
Example of anaerobic photoautotrophs
Definition
green bacteria
Term
Where does green bacteria get its electrons?
Definition
from hydrogen sulfide and hydrogen gas.
Term
Chemoautotrophic bacteria
Definition
Influence global cycling of nitrogen, sulfur and other nutrients. Nitrifying bacteria in the soil obtain electrons from ammonia.
Term
What do chemoautotrophic bateria provide for plants?
Definition
nitrate.
Term
chemoheterotrophic bacteria
Definition
nearly all known bacterial species are in this category. Many are decomposers.
Term
Lactobacillus
Definition
chemoheterotrphic bacteria. used to make pickles, buttermilk, sauerkraut, yogurt.
Term
actinomycetes
Definition
chemoheterotrophic bacteria. Sources of antibiotics.
Term
E. coli
Definition
chemoheterotrophic bacteria. Makes vitamin K and helps stop many food-borne pathogens from colonizing the gut.
Term
Azospirillum
Definition
chemoheterotrophic bacteria. Sugarcane and corn benefit from this nitrogen-fixing sirochete.
Term
Deinococcus radiodurans
Definition
chemoheterotrophic bacteria. resists high radiation doses that kill other species.
Term
Deinococcus radiodurans
Definition
chemoheterotrophic bacteria. resists high radiation doses that kill other species.
Term
Some examples of pathogenic chemoheterotrophic bacteria?
Definition
E. coli (causes a form of diarrhea that is the main cause of infant death in developing countries), Clostridium botulinum (a food taint whose toxins cause botulism). C. tetani (causes the diease tetanus)
Term
How does the C. tetani work?
Definition
It can form an endospore.
Term
endospore
Definition
This resting structure forms inside the cell, around one copy of the bacterial chromosome and part of the cytoplasm. Endospores form when a depletetion of nitrogen arrests cell growth. They are released as free spores when the plasma membrane erupts.
Term
Why are endospores so dagerous?
Definition
They are resistant to heat, drying out, irradiation, acids, disinfectanats, and boiling water. They can stay dormant, in some cases, for many decades.
Term
Examples of pathogenic chemoheterotrophic bacteria that travels from host to host in the gut of insects:
Definition
Borrelia burgdorferi (causes lyme disease) Rickettsia rickettsii (causes Rocky Mountain Fever)
Term
Describe what happens to you when you are bitten by a tick that has Rickettsia rickettsii in its gut:
Definition
After the bacterium enters the host, it penetrates the cytopasm and nucleus of the hosts cells. 3-12 days later, a fever and severe headache develop. 3-5 days later, a rash appears on extremities. Diarrhea and gastrointestinal cramps are common.
Term
What facts point to the idea that bacteria are small but not simple?
Definition
Their behavior. They will move toward nutrient-rich regions. Aerobes move toward oxygen, and anaerobes move away from it. photosynthetic types move toward light, but will move away if the light gets too intense.
Term
Magnetotactic bacteria
Definition
contain a chain of magnetite particles that serve as a tiny compass which helps them sense which way is north and also down. They swim towards the bottom of a body of water, where oxygen levels are lower and therefore more suitable for their growth.
Term
An example of collective behavior of bacteria:
Definition
millions of Myxococcus xanthus cells form a predatory colony. These cells secrete an enzyme that digests prey such as syanobacteria, that become stuck to the colony. Bacteria have also been known to migrate together, then all change direction to move toward a food source.
Term
fruiting bodies
Definition
spore-bearing structures
Term
how do Myxobacteria form fruiting bodies?
Definition
some cells in the colony differntiate and form a slime stalk, others form branches, and others form clusters of spores. The spores disperse when a cluster bursts open, each may give rise to a new colony.
Term
what does the word virus mean?
Definition
poison or venomous secretion
Term
What is a virus?
Definition
A noncellular infectious agent that has two characteristics. 1) a viral particle consists of a protein coat wrapped around a nucleic acid core (its genetic material) and 2) It can be reproduces only after its genetic material enters a host cell.
Term
Is the genetic material in a virus DNA or RNA?
Definition
It can be either.
Term
What is the purpse of the protein coat around the virus?
Definition
It helps protect the genetic material on the journey to the host cell. Its contains proteins that can bind with specific receptors on host cells. It also contains spikes of glycoproteins, which spike when a predator tries to engulf it.
Term
How can viruses often avoid immune fighters?
Definition
Their genes mutate at high frequencies. That is why the flu shot is different every year.
Term
What do we study to learn more about viruses?
Definition
bacteriophages
Term
Why is it almost impossible to study the virus itself?
Definition
Because each kind of virus can multiply only in certain hosts. It cannot be studied easily unless the investigator cultures living hosts.
Term
bacteriophages
Definition
a group of viruses that infect bacterial cells.
Term
How big are animal viruses?
Definition
They range in size from parvoviruses (18 nanometers) to brick-shaped poxviruses (350 nanometers).
Term
What obstacles must viruses overcome in order to infect a plant? How do they overcome this obstacle?
Definition
They must first breach the cell wall. They typically hitch rides on the piercing or sucking devices of insects that feed on insect juices.
Term
Example of a virus that infects plants?
Definition
tobacco mosaic virus (RNA virus)
Term
What are the four steps all viruses go through when they multiply?
Definition
1) Attachment
2) Penetration
3) replication and synthesis
4) assembly
Term
At what stage does the way viruses multiply branch into two different paths?
Definition
at step 5, Release.
Term
Attachment stage of viral multiplication:
Definition
A virus attaches to a host cell by molecular groups that can chemically recognize and lock on to specific molecular groups at the cells surface.
Term
Penetration stage of viral multiplication:
Definition
Either the whole virus or its genetic material alone penetrates the cells cytoplasm.
Term
Replication and synthesis stage of viral multiplication:
Definition
In an act of molecular piracy, the viral DNA or RNA directs the host cell into producing many copies of the viral nucleic acids and viral proteins, including enzymes.
Term
Assembly stage of viral multiplication:
Definition
The viral nuceic acids and viral proteins become organized as new infectious particles.
Term
Lytic pathway:
Definition
Steps 1-4 proceed rapidly, and new particles are released when a host cell undergoes lysis.
Term
Lysongenic pathway
Definition
A latent period extents the cycle. The virus doesn't kill the host cell outright. a viral enzyme cuts the host chromosome, then integrates viral genes into it. When the infected cells prepares to divide, it replicates the recombinant molecule. Miniature time bombs are passed on to all of the cells descendants. Later on, a molecular signal of some other stimulus may reactivate the cycle.
Term
An example of a virus that has a latency period:
Definition
Type I herpes simplex virus. Almost everyone harbors this virus, it remains latent in facial tissues inside a ganglion. Sunburn and other stress factors can make it come out.
Term
Example of a retrovirus and how it works:
Definition
HIV. Carts its own enzymes into ells. These assemble Dna on viral RNA by reverse transcriptase.
Term
Viroids
Definition
tightly folded strands or circles of RNA are smaller than anything in viruses. They too cause infections even though they do not have protein-coding genes.
Term
do viroids have a protein coat?
Definition
no, but the tight folding might help protect them from the hosts enzymes.
Term
What are some theories about where viroids come from?
Definition
They resemble introns (the noncoding portions of eukaryotic DNA). They might be self-splicing introns that escaped from DNA molecules. Or they may be remnants left over from an ancient RNA world.
Term
Prions
Definition
are largely, if not entirely, abnormal, less soluble forms of proteins necessary for the operation of neurons (the communication cells of the nervous system).
Term
what do prions do?
Definition
They catalyze the conversion of the normal proteins into more prions. They coagulate as massive deposits inside the brain and cause fatal degenerative diseases.
Term
Creutzfeldt-Jakob disease
Definition
disease that progressively destroy muscle coordination and brain function. Caused by prions.
Term
scrapie
Definition
a prion-linked disease of sheep. The sheep scratch themselves against trees or posts until all of their wool is gone.
Term
BSE
Definition
Bovine spongiform encephalopathy. (mad cow disease) Prion-linked.
Term
How did the first case of BSE come about?
Definition
Sheep with scrapie were ground up and feed to cows.
Term
infection
Definition
when a pathogen invades yout body, multiplies in cells and tissues.
Term
disease
Definition
the outcome of infection. occurs when defenses cannot be mobilized fasr enough to keep the pathogens activities from interfering with body functions.
Term
epidemic
Definition
a disease spreads fast through part of a population for a limited time, then subsides.
Term
pandemic
Definition
If an epidemic breaks out in several countries at the same time (example: AIDS)
Term
Sporadic diseases
Definition
(such as whooping cough) occur irregularly and effect few people.
Term
Endemic diseases
Definition
pop up more or less continuously but do not spread far in large populations. (example: T.B. and impetigo)
Term
Two barriers that prevent pathogens from evoloving into a position of world dominance:
Definition
any species with a history of being attacked by a certain pathogen has coevolved with it and has built defenses against it. And if a pathogen kills too quickly, it might disappear along with the host.
Term
When will a pathogen cause an individual to die?
Definition
only if it becomes host to overwhelming number of pathogens, if it is a novel host with no coevolved defenses, or if a mutant pathogenic strain has emerged and has breached current defenses.
Term
emerging pathogens
Definition
have been around for a long time and are only now taking advantage of the presence of novel human hosts. Others are newly mutated strains of existing pathogens.
Term
Ebola
Definition
one of several viruses that cause a deadly hemorrhagic fever. It may have coevolved with monkeys in africa tropical forests. By 1976, it was infecting humans.
Term
monkeypox virus
Definition
a relative of the smallpox virus. after infecting its host, this DNA virus replicates in bone marrow, the lymph nodes and the spleen. The blood stream transports new viral particles to the skin where they cause large, hardened pus-filled sores.
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