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Definition
| DNA in which genes from two different sources are linked |
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Term
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Definition
| direct manipulatin of genes for practical purposes |
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Term
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Definition
| manipulation of organisms or their components to perform practical tasks or provide useful products |
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Definition
| bacterial enzymes that cut foreign DNA |
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Definition
| recognition sequence of nucleotides that restriction enzymes recognize and cut |
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Term
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Definition
| pieces of DNA left over after it is cut by the restriction enzyme |
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Definition
| enzyme that forms phosphodiester bonds between nucleotides in effect making longer strands out of short strands |
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Definition
| single stranded end of a DNA fragment that has been cut by a restriction enzyme |
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Definition
| DNA molecule that can carry foreign DNA into a cell and replicate there |
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Term
| nucleic acid hybridization |
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Definition
| base pairing between a gene and a complementary sequence on another nucleic acid molecule |
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Definition
| short single - stranded nucleic acid that binds to a complementary gene and tags it |
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Definition
| separation of the two strands of DNA |
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Definition
| cloning vector that carries the requisite prokaryotic promoter sequence upstream of the restriction site where the eukaryotic gene will be inserted |
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Definition
| DNA for a gene with no introns-made from the mRNA of the gene and then transcribed using reverse transcriptase |
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Definition
| application of a brief electrical pulse to a solution containing cells that will allow them to uptake DNA easier |
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Definition
| complete set of thousands of recombinant plasmid clones from the original genome of interest |
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Definition
| genomic copies of only the genes that were transcribed in the starting cells (partial genome) |
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Term
| PCR-Polymerase Chain Reaction |
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Definition
| rapid amplification of a piece of DNA without using cells |
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Term
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Definition
| technique that separates macromolecules (nucleic acids or proteins) on the basis of size, electrical charge and other physical properties |
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Definition
| technique that detects specific DNA sequences in DNA samples and the number of such sequences and the size of the restriction fragment that contains them |
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Term
| RFLP-restriction length polymorphisms |
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Definition
| differences in DNA sequence on homologous chromosomes that result in different size restriction fragments |
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Term
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Definition
| radioactive probe base pairs with complementary sequences in denatured DNA of intact chromosomes on a microscope slide |
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Definition
| international effort to map the entire human genome (nucleotide sequence of all 23 chromosomes |
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Term
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Definition
| microscope slides containing many single stranded DNA fragments representing different genes (ideally all the genes of an organism)used to study gene expression |
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Definition
| introduction mutations into the sequence of cloned DNA. return the gene to the original organism and study the effect |
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Term
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Definition
| study of genomes and genes based on DNA sequencing |
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Term
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Definition
| harmless variant or derivative of a pathogen that stimulates the immune system to fight the pathogen |
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Term
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Definition
| pattern of RFLP bands that are unique to each person and used for identification |
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Term
| STR (single tandem repeats) |
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Definition
| repeated nucleotide sequences in the genome that vary from person to person |
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Term
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Definition
| organisms that contain genes from another organism |
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