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Chapter 1 Theory
Test 1
28
Biology
Undergraduate 3
09/13/2013

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Term
Microbiology
Definition
The study of Microbes
Term
Microbes
Definition
Forms of life too small to be seen with the naked eye
Term
"Viable but not culturable"

Why?
Definition
Alive but we can't grow it in the lab.

We might not have the right medium or be able to isolate it.
Term
Characteristics of Life (9)
Definition
Metabolism, growth, reproduction, differentiation, communication, movement, genetic variation/evolution, response/adaptation to the external environment, and homeostasis.
Term
Why aren't viruses considered alive?
Definition
Don't replicate outside of a host cell
Usually little to no biochemical activitiy outside of a host cell
Inert & nonreactive to environment
Term
Bacteriophage
Definition
Viruses that invade bacteria
Term
What macromolecules are necessary for life?
Definition
Polypeptides, nucleic acids, lipids, polysaccharides
Term
Polypeptides necessary for life
Definition
Amino acids
Enzymes that catalyze the vast majority of biochemical reactions in the cell. Other proteins are structural components of cells.
Weight of Cell: 50-55%
Term
Nucleic acids necessary for life
Definition
Deoxyribonucleotides
->For DNA; genes. Weight of Cell: 2-5%
Ribonucleotides
->For RNA; produce polypeptides. Weight of Cell: 15-20%
Term
Lipids necessary for life
Definition
Diverse structures
Makes up cellular membranes that form physical boundary between the inside of cell and surroundings and membranes of internal organelles.
Weight of Cell: 10%
Term
Polysaccharides necessary for life
Definition
Sugars
Structural and energy storage
Weight of Cell: 6-7%
Term
How did microbial life arise?
Definition
Early conditions formed RNA molecuels & micelles.
Came together into a primitive cell for storing genetic information and coding.
Primitive cells eventually switched from RNA to DNA.
The micelle may have been the early version of the plasma membrane.
Term
How did eukaryotes appear?
Definition
Endosymbiotic Theory
Primitive prokaryotes ingested other microbes.
Mitochondria - respiration
Chloroplasts - taking in light energy
Term
What are the two perspectives we use to examine microbial genomes?
Definition
1. Examining effects on single mutations in DNA individually.
2. Studying and comparing pieces of genomes to each other (bioinformatics) across domains.
Term
Three Domains
Definition
Bacteria, Archaea, Eukarya
Term
PCR
Definition
Enhanced the spread and ease of doing DNA sequence examinations. Helps compare and contrast organisms in different domains.
Term
How does the studying of genetic microbes help humans?
Definition
Altering the genomes of microbes allows us to mass-produce molecules that humans want.
Example: the production of human insulin by inserting the gene into E.Coli cells
Term
Normal flora
Definition
What is normally in our gut.
Differs by age.
Influences health. Ex: prevents Clostridium difficile.
Term
Types of media
Definition
Solid, Liquid, Semi-fluid
Term
Sterile
Definition
Devoid of all living organisms
Liquid is clear during this stage
Term
Turbid
Definition
Descripes liquid that is not sterile; cloudy
Term
Spore
Definition
Fungi use it to survive
Term
Endospore
Definition
Bacteria use it to survive
Term
Petri Dish
Definition
A type of vehicle for solid agar
Term
Koch's Postulates
Definition
1. The suspected pathogenic organism should be present in all cases of the disease and absent from healthy animals.
2. The suspected oranism should be grown in pure culture.
3. Cells from a pure culture of the suspected organism should cause disease in a healthy animal.
4. The organism should be reisolated and shown to be the same as the original.
Term
Types of algar
Definition
Nutrient algar
Triptic soy algar (higher quality)
Term
Four-column streak plate
Definition
Using inoculating tool (needle or loop)
4 quadrants
To grow colonies started by a single cell
Term
What has caused a reduction in deaths from disease?
Definition
Antispetics
Sanitation improvements
Food/water safety
Personal hygiene improvements
Vaccination
Treatment of infections
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