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Apparatus of gene expression in Bacteria |
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RNA polymerase, Ribosomal RNAs, and Translation factors |
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In order: Domain Phylum Class Order Family Genus Species (Think PCOFGS). |
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phylum of bacteria with classes: alpha, beta, gamma, delta and epsilon |
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bacteria, archaea, eukarya |
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Genus: escherichia
species: coli |
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Deep branching thermophiles |
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- grow at temperatures up to 95 degrees C
- show fastest doubling rates of all cells
- born, grow and reproduce in 10 minutes
- high rates of mutation
- accelerated molecular clocks
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Deep-branching thermophiles (Part 2) |
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Definition
-genomes show extensive transfer of archaeal genes -Thermocrinis ruber
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Forms filamentous mats in hydrothermal vents |
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play key roles in many ecosystems; environmental change may cause colonial cyanobacteria to form giant blooms
include: prochlorococcus (phototroph in ocean) |
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two distinct phylogenetic branches: 1. phylum firmicutes 2. phylum actinobacteria |
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LOW G/C species (35% GC, 65% AT) |
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HIGH GC Species (35%AT, 65% GC) |
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Firmicutes and Actinobacteria |
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both have thick cell walls that retain gram stains; walls are reinforced by teichoic acids |
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Order: Bacillales Genus: Bacillus Large, rod-shaped cells Ex: Bacillus anthracis
Vegetative cells develop inert dendospores in time of starvation and stress |
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Order: Clostridiales Genus: Clostridium Endospore swells, forming a 'drumstick' Examples: C. botulinum, C. tetani, C. difficile
Known: Botox (used to relax muscle spasms) |
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Order: Clostridiales E. fishelsoni Large, can be seen with naked eye. Grows in GI tract of sturgeonfish Gives live birth to two internal offspring |
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Non-Spore forming Firmicutes |
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Listeria monocytogenes (facultative anaerobic rod that causes gastroenteritis) |
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Nonspore forming firmicute; facultative anaerobes cocci in clusters example: S. aureus |
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Nonspore forming firmicute; cocci in chains Ex: S. pneumoniae |
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In phylum Actinobacteria; an acid fast organism; example: Streptomyces (major source of antibiotics) that form arthrospores |
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mycelia that fragment into smaller cells |
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nonmycelial actinobacteria |
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rod shaped cells with thick cell walls containing mycolic acids and phenolic glycolipids
Ex: m. tuberulosis m. leprae m. smegmatis |
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consist of five major classes; all have triple-layered gram negative cell envelope (outer membrane, periplasm(cell wall) and cell membrane) |
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Endosymbionts (nitrogen fixers and plant roots like rhizobia) Ricksettsias (intracellular pathogens spread by ticks) |
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Lithotrophs and pathogens |
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nitrifiers oxidize ammonia to nitrite ex: nitrosomonas: used in wastewater treatment |
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Neisseria gonorrhoeae in humans |
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Enterobacteriaceae and aerobic rods |
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rods
grow singly, in chains or in biofilms
motile with flagella
aerobic or anaerobic respiration
ferment rapidly on carbs ex: Escherichia coli |
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Pseudomonas species have polar flagella and are vigorous swimmers; can develop biofilms
Legionella pneumophila grows intracellularly within macrophages and amebas |
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parastizes proteobacterial cells, penetrates into periplasm where it uses host resources to grow |
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microaerophilic helical pathogens Example: Helicobacter pylori causes gastritis and stomach ulcers; converts uera to ammonia and CO2 |
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unique phylum of heterotrophic bacteria consisting of sheathed spiral cells with internal flagella Example: Treponema pallidum (syphilis) Borrelia burgdorferi (lyme disease) |
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