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cells from one strain produced a heritable change in another strain |
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Hershey- Chase experiment DNA was labeled with |
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Hereshey-Chase experiment proteins labeled with |
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genetic transformation of eukaryotic cells |
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Definition
a gene that confers an observable phenotype |
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Definition
found the structure of DNA using x-ray crystallography |
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Definition
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deoxyribose, phosphate group, nitrogen containing base |
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the number of purines = the number of pyrimidines |
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Definition
built the DNA structure model |
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Definition
polynucleotide chains ran in opposite directions |
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Definition
-double stranded helix w/ uniform diameter -right handed -antiparallel -nitrogenous bases are exposed in major and minor grooves |
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has a free phosphate group |
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Definition
has a free hydroxyl group |
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Definition
-store genetic info -genetic material able to mutate -genetic material is replicated in cell division -genetic material expressed as phenotype |
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Term
T or F Dna serves as a template for synthesis of DNA |
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Definition
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Definition
parent serves as a template and new molecules have one old and one new strand |
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Definition
original helix only serves as a template |
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parent fragments serve as templates, assembling old and new parts into molecules |
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Definition
join the nucleotides added to the new strand |
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helps increase DNA replication |
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Definition
starts DNA replication and is made up of RNA |
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adds nucleotides to the 3 prime end |
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Definition
is the site where DNA unwinds to expose bases |
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Definition
uses energy from ATP hydrolysis to unwind the DNA |
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single-stranded binding proteins |
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Definition
keep the strands from getting back together |
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Definition
is oriented to grow at its 3 prime end as the fork opens |
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Definition
oriented so the 3 prime end gets farther from the fork |
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small discontinuous stretches of the lagging strands |
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Definition
adds nucleotides to the 3 prime end |
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Definition
replaces the primer with DNA |
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Definition
catalyzes the phosphodiester linkages |
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Term
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Definition
catalyze many polymerizations each time they bind to DNA |
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Definition
separates the two chromosomes |
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Term
T or F linear DNA have one origin of replication |
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Definition
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repetitive sequences at the end of eukaryotic chromosomes |
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catalyzes addition of lost telomeric DNA sequences |
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if bases are paired incorrectly, the nucleotide is removed |
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detects mismatched bases on new strands that have not yet been modified |
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enzymes constantly scan DNA for damaged bases, damaged bases are excised and DNA polymerase I adds correct ones |
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polymerase chain reaction (PCR) |
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Definition
make copies of DNA sequences in a cyclical process |
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Definition
separates the DNA into 2 strands |
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