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Chapter 13
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43
Biology
Undergraduate 1
11/15/2010

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Term
transforming principle
Definition
cells from one strain produced a heritable change in another strain
Term
Hershey- Chase experiment DNA was labeled with
Definition
32P
Term
Hereshey-Chase experiment proteins labeled with
Definition
35S
Term
transfection
Definition
genetic transformation of eukaryotic cells
Term
genetic marker
Definition
a gene that confers an observable phenotype
Term
Rosalind Franklin
Definition
found the structure of DNA using x-ray crystallography
Term
Purines
Definition
adenine, guanine
Term
pyrimidines
Definition
cytosine, thymine
Term
DNA nucleotides
Definition
deoxyribose, phosphate group, nitrogen containing base
Term
Chargaff's rule
Definition
the number of purines = the number of pyrimidines
Term
Crick and Watson
Definition
built the DNA structure model
Term
antiparallel
Definition
polynucleotide chains ran in opposite directions
Term
Four key features of DNA
Definition
-double stranded helix w/ uniform diameter
-right handed
-antiparallel
-nitrogenous bases are exposed in major and minor grooves
Term
5 prime end
Definition
has a free phosphate group
Term
3 prime end
Definition
has a free hydroxyl group
Term
4 Functions of DNA
Definition
-store genetic info
-genetic material able to mutate
-genetic material is replicated in cell division
-genetic material expressed as phenotype
Term
T or F Dna serves as a template for synthesis of DNA
Definition
True
Term
Semiconservative
Definition
parent serves as a template and new molecules have one old and one new strand
Term
conservative
Definition
original helix only serves as a template
Term
dispersive
Definition
parent fragments serve as templates, assembling old and new parts into molecules
Term
phosphodiester linkages
Definition
join the nucleotides added to the new strand
Term
replication complex
Definition
helps increase DNA replication
Term
primer
Definition
starts DNA replication and is made up of RNA
Term
DNA polymerase
Definition
adds nucleotides to the 3 prime end
Term
replication fork
Definition
is the site where DNA unwinds to expose bases
Term
DNA helicase
Definition
uses energy from ATP hydrolysis to unwind the DNA
Term
single-stranded binding proteins
Definition
keep the strands from getting back together
Term
leading strand
Definition
is oriented to grow at its 3 prime end as the fork opens
Term
lagging strand
Definition
oriented so the 3 prime end gets farther from the fork
Term
Okazaki Fragments
Definition
small discontinuous stretches of the lagging strands
Term
DNA polymerase III
Definition
adds nucleotides to the 3 prime end
Term
DNA polymerase I
Definition
replaces the primer with DNA
Term
DNA Ligase
Definition
catalyzes the phosphodiester linkages
Term
processive
Definition
catalyze many polymerizations each time they bind to DNA
Term
DNA topoisomerase
Definition
separates the two chromosomes
Term
T or F linear DNA have one origin of replication
Definition
False
Term
Telomerase
Definition
repetitive sequences at the end of eukaryotic chromosomes
Term
Telomerase
Definition
catalyzes addition of lost telomeric DNA sequences
Term
Proofreading
Definition
if bases are paired incorrectly, the nucleotide is removed
Term
mismatch repair
Definition
detects mismatched bases on new strands that have not yet been modified
Term
excision repair
Definition
enzymes constantly scan DNA for damaged bases, damaged bases are excised and DNA polymerase I adds correct ones
Term
polymerase chain reaction (PCR)
Definition
make copies of DNA sequences in a cyclical process
Term
Denaturation
Definition
separates the DNA into 2 strands
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