Term
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Definition
| Biomanipulation of organisms to perform practical tasks for humans |
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Definition
| Technology: Creates Recombinant Plasmid w/ restriction enzymes |
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Term
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Definition
| Transfer of DNA (donar to recipient) through a SEX PILLUS |
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Term
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Definition
| Uptake of naked foreign DNA by host bacterium (no bridge or virus) |
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Term
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Definition
| Transfer of foreign DNA by a VIRAL vector |
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Definition
| Microbiologist; 1st to discover TRANSFORMATION (used mice) |
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Definition
| DNA from two different organisms spliced together |
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Term
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Definition
| Extrachromosomal, circular piece of DNA in bacteria (survival) |
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Definition
| Name for PLASMID with foreign DNA spliced into it |
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Term
| Restriction Enzyme (1971) |
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Definition
| Bacterial enzyme prevents phage DNA from replacing (key to Genetic Engineering) |
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Term
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Definition
| Restriction site; complimentary BP's run in reverse (5-6 BP in length) |
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Term
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Definition
| Restriction enzymes cut in ways that leave exposed single strands |
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Term
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Definition
| Enzyme that JOINS sticky ends by Complementary base pairing |
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Term
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Definition
| Complete collection of cloned DNA fragments from an organism |
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Term
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Definition
| Genetically Modified Organism w/ new artificial genes (pest/herb resist) |
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Definition
| Organism (cow/pig) carrying specific genes from another species |
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Term
| Agrobafctyerium tumefaciens |
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Definition
| Plant bacterium; causes "tumors"; R-plasmid used to inject desired gene |
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Term
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Definition
| Characteristic used to SELECT bacteria with recombinant plasmid |
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Term
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Definition
| Duplication of identical genes in bacteria (through PLASMID transfer) |
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Term
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Definition
| identical animal created from donar nuclei replacing host enucleated egg |
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Term
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Definition
| Viral genes CLONED in yeast cells -> mass produced viral antigens |
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Term
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Definition
| Sometimes called a VECTOR; carries foreign DNA into host |
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Term
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Definition
| Carries desired genes (called "passenger"); it replicates in HOST cell |
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Term
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Definition
| Polymerase Chain Reaction; mass produces small DNA pieces in test tube |
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Term
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Definition
| Technique: Separates DNA fragments (RFLPs) by length (# BPs) |
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Term
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Definition
| Technique: Replaces "BAD" genes with "GOOD" genes |
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Term
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Definition
| Technique: Develops bands from RFLPs; associated most with forensics |
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Term
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Definition
| Exposing Electrophoretic Markers to X-ray film -> banding pattern |
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Term
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Definition
| Complementary radioactively labeled DNA used to find desired gene |
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Term
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Definition
| Primary bacterium used in Genetic Engineering |
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Term
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Definition
| Characteristic commonly used to assess uptake of R-plasmid with desired gene |
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