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Chapter 11- Biotech and DNA
BIO 111 Exam 5
50
Biology
Undergraduate 1
08/13/2013

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Term
WHAT IS A GENE MUTATION?
Definition
CHANGE IN THE SEQUENCE OF BASES IN A GENE
Term
WHAT 3 THINGS CAUSE MUTATIONS?
Definition

1. REPLICATION ERRORS

2. TRANSPOSONS

3. MUTAGENS

Term

REPLICATION ERRORS, TRANSPOSONS, OR MUTAGENS?

"JUMPING GENES"

Definition
TRANSPOSONS
Term

REPLICATION ERRORS, TRANSPOSONS, OR MUTAGENS?

RARE DUE TO PROOFREADING OF DNA

Definition
REPLICATION ERRORS
Term

REPLICATION ERRORS, TRANSPOSONS, OR MUTAGENS?

CAN BE ENVIRONMENTAL OR CHEMICAL

Definition
MUTAGENS
Term

REPLICATION ERRORS, TRANSPOSONS, OR MUTAGENS?

RADIATION

Definition
MUTAGENS
Term

REPLICATION ERRORS, TRANSPOSONS, OR MUTAGENS?

PIECES OF DNA THAT MOVE WITHIN AND BETWEEN CHROMOSOMES

Definition
TRANSPOSONS
Term

TRUE OR FALSE:

MANY MUTATIONS GO UNDETECTED AND HAVE NO OBSERVABLE EFFECT.

Definition
TRUE
Term
2 TYPES OF MUTATIONS?
Definition

1. POINT

2. FRAMESHIFT

Term

POINT OR FRAMESHIFT MUTATION?

CHANGE IN SINGLE DNA NUCLEOTIDE.

Definition
POINT
Term

POINT OR FRAMESHIFT MUTATION?

EXTRA OR MISSING NUCLEOTIDES.

Definition
FRAMESHIFT
Term

POINT OR FRAMESHIFT MUTATION?

USUALLY MUCH MORE SEVERE.

Definition
FRAMESHIFT
Term

POINT OR FRAMESHIFT MUTATION?

ALL DOWNSTREAM CODONS AFFECTED.

Definition
FRAMESHIFT
Term

POINT OR FRAMESHIFT MUTATION?

RESULTS CAN BE MINOR OR SEVERE.

Definition
POINT
Term

POINT OR FRAMESHIFT MUTATION?

EXAMPLE IS SICKLE CELL DISEASE.

Definition
POINT
Term

POINT OR FRAMESHIFT MUTATION?

REMOVE CYTOSINE (C).

Definition
FRAMESHIFT
Term
WHAT IS GENETIC ENGINEERING?
Definition
INSERTING A CLONED GENE INTO AN ORGANISM.
Term
WHAT IS THE RESULT OF GENETIC ENGINEERING?
Definition
A TRANSGENIC ORGANISM
Term
WHAT IS IT CALLED WHEN AN IDENTICAL COPY IS PRODUCED FROM GENETIC ENGINEERING?
Definition
CLONE
Term

TRUE OR FALSE:

B/C THE GENETIC CODE IS NEARLY UNIVERSAL, IT'S POSSIBLE TO TRANSFER CLONED GENES BETWEEN VIRTUALLY ANY ORGANISM.

Definition
TRUE
Term
WHAT IS RECOMBINANT DNA?
Definition
COMBINES DNA FROM 2 OR MORE ORGANISMS = rDNA
Term
HOW IS rDNA MADE?
Definition

1. A VECTOR IS USED TO CARRY THE FOREIGN DNA; MAY BE IN THE FORM OF A PLASMID

2. RESTRICTION ENZYMES CUT DNA AT SPECIFIC SITES

3. DNA LIGASE IS USED TO FUSE THE CUT PIECES TOGETHER

Term

TRUE OR FALSE:

HUMAN INSULIN IS MADE BY USING BACTERIAL CELLS.

Definition
TRUE
Term
HOW IS HUMAN INSULIN MADE?
Definition
BY USING RECOMBINANT DNA TECHNOLOGY- HUMAN GENE REMOVED AND INSERTED INTO A PLASMID, THEN BACTERIA PRODUCES INSULIN AS IF IT WAS ONE OF THEIR OWN PRODUCTS
Term
EXAMPLES OF TRANSGENIC PLANTS?
Definition
COTTON, CORN, SOYBEANS, AND POTATOES
Term
EXAMPLES OF TRANSGENIC ANIMALS?
Definition
FISH, COWS, AND PIGS
Term
WHAT IS THE BENEFIT TO TRANSGENIC COTTON, CORN, AND POTATOES?
Definition
THEY HAVE BEEN GENETICALLY MODIFIED TO PRODUCE THEIR OWN INSECTICIDE
Term
WHAT IS THE BENEFIT OF TRANSGENIC SOYBEANS?
Definition
THEY HAVE BEEN GENETICALLY MODIFIED TO BE RESISTANT TO HERBICIDES
Term
WHAT DOES PCR STAND FOR?
Definition
POLYMERASE CHAIN REACTION
Term
WHAT IS PCR USED FOR?
Definition
IT AMPLIFIES SPECIFIC DNA SEQUENCES FOR STUDY
Term
WHAT IS DNA POLYMERASE?
Definition
IT MAKES DNA AND CAN TOLERATE HIGH TEMPERATURES
Term
WHAT ARE PRIMERS?
Definition
SPECIFIES WHICH SPECIFIC DNA SEGMENT IS TO BE AMPLIFIED; ONLY AMPLIFIES TARGET
Term
HOW DOES PCR WORK?
Definition
AMPLIFIES SEGMENTS OF DNA USING PRIMERS, MAKES COPIES OF THE AMPLIFIED SEGMENT USING DNA POLYMERASE, THEN INCREASING THE AMOUNT OF DNA TO BE STUDIED WITH EACH CYCLE
Term
WHAT IS DNA FINGERPRINTING?
Definition
MAKES USE OF REPEATING NONCODING DNA SEGMENTS
Term
HOW DO OUR DNA FINGERPRINTS DIFFER?
Definition
PEOPLE DIFFER IN HOW MANY REPEATS OF NONCODING DNA THERE ARE
Term
FOR A CLOSER LOOK AT DNA FINGERPRINTS, WHAT CAN BE USED?
Definition
PCR
Term
TO SEPARATE DNA SAMPLES BY SIZE, WHAT CAN BE USED?
Definition
ELETROPHORESIS- LONGER DNA STRANDS ARE LARGER AND MIGRATE LESS ON THE GEL
Term
WHAT IS THE STUDY OF GENOMES CALLED?
Definition
GENOMICS
Term
WHAT IS THE HUMAN GENOME PROJECT?
Definition
A 13 YEAR EFFORT THAT RESULTED IN FINDING MANY SMALL REGIONS OF DNA THAT VARY AMONG INDIVIDUALS
Term
WHAT ARE 2 THINGS THAT THE DISCOVERIES OF THE HUMAN GENOME PROJECT TELL US?
Definition

1. SOME INDIVIDUALS EVEN HAVE EXTRA COPIES OF GENES

2. DIFFERENCES MAY HAVE NO EFFECT OR MAY INCREASE OR DECREASE ONE'S SUSCEPTIBILITY TO DISEASE

Term

TRUE OR FALSE:

THE HUMAN GENOME PROJECT CAN GIVE US CLUES TO EVOLUTIONARY ORIGINS.

Definition
TRUE
Term

TRUE OR FALSE:

HUMANS AND CHIMPS ARE 85% ALIKE GENETICALLY.

Definition
FALSE- HUMANS AND CHIMPS ARE 98% ALIKE GENETICALLY
Term

TRUE OR FALSE:

HUMANS AND MICE ARE 50% ALIKE GENETICALLY.

Definition
FALSE- HUMANS AND MICE ARE 85% ALIKE GENETICALLY.
Term

TRUE OR FALSE:

HUMANS ALSO SHARE GENES WITH BACTERIA.

Definition
TRUE
Term
AMONG THE GENES THAT DIFFERED BETWEEN HUMANS AND CHIMPS, CHROMOSOME ______ IS ESPECIALLY IMPORTANT.
Definition
22
Term
COMPARING GENOMES MAY BE A WAY OF FINDING GENES ASSOCIATED WITH HUMAN D__________.
Definition
DISEASES
Term
PROTEOMICS
Definition
EXPLORES STRUCTURE AND FUNCTION OF CELLULAR PROTEINS AND HOW THEY INTERACT TO PRODUCE TRAITS
Term
WHAT AREA OF STUDY IS PROTEOMICS ESPECIALLY IMPORTANT TO?
Definition
DRUG DEVELOPMENT
Term
BIOINFORMATICS
Definition
APPLICATION OF COMPUTER TECHNOLOGIES TO STUDY GENOME AND PROTEOME
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