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Chapter 10
Molecular Biology of the Gene
36
Biology
11th Grade
01/28/2010

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Term
bacteriophages "phages"
Definition
a virus that infects bacteria
Term
nucleotides
Definition
an organic monomer consisting of a five carbon sugar covalently bonded to a nitrogenous base and a phosphate group. the building blocks of nucleic acids
Term
polynucleotide
Definition
a polymer made up of many nucleotides covalently bonded together
Term
sugar-phosphate backbone
Definition
the alternating chain of sugar and phosphate to which the DNA and RNA nitrogenous bases are attached
Term
pyrimidines
Definition
single ring structures--thymine (t) and cytosine (c)
Term
purines
Definition
double-ring structures--Adenine (A) and guanine (G)
Term
double helix
Definition
the form of native DNA, referring to its two adjacent polynucleotide strands wound into a spiral shape
Term
dna polymerases
Definition
an enzyme that assembles DNA nucleotides into polynucleotides using a preexisting strand of DNA as a template
Term
dna ligase
Definition
an enzyme, essential for DNA replication, that catalyzes the covalent bonding of adjacent DNA nucleotides; used in genetic engineering to paste a specific piece of DNA containing a gene of interest into a bacterial plasmid or other vector
Term
transcription
Definition
the synthesis of RNA on a DNA template.
Term
translation
Definition
the synthesis of a polypeptide using the genetic information encoded in an mRNA molecule. there is a change of language from nucleotides to amino acids.
Term
triplet code
Definition
a set of three nucleotide-long words that specify the amino acids for polypeptide chains
Term
codon
Definition
a three nucleotide sequence in mRNA that specifies a particular amino acid or polypeptide termination signal; the basic unit of the genetic code
Term
RNA polymerase
Definition
an enzyme that links together the growing chain of RNA nucleotides during transcription, using a DNA strand as a template
Term
promoter
Definition
a specific nucleotide sequence in DNA, located at the start of a gene, that is the binding site for RNA polymerase and the place where transcription begins
Term
terminator
Definition
a special sequence of nucleotides in DNA that marks the end of a gene; it signals RNA polymerase to release the newly made RNA molecule which then departs from the gene.
Term
messenger RNA (mRNA)
Definition
the type of ribonucleic acid that encodes genetic information from DNA and conveys it to ribosomes, where the information is translated into amino acid sequences
Term
RNA splicing
Definition
the removal of introns and joining of exons in eukaryotic RNA, forming an mRNA molecule with a continuous coding sequence; occurs before mRNA leaves the nucleus
Term
intron
Definition
in eukaryotes, a non-expressed (noncoding) portion of a gene that is excised from the RNA transcript
Term
exon
Definition
in eukaryotes, a coding portion of a gene
Term
transfer RNA (tRNA)
Definition
a type of ribonucleic acid that functions as an intrepreter in translation. each tRNA molecule has a specific anticodon, picks p a specific amino acid, and coneys the amino acid to the appropriate codon on mRNA
Term
anticodon
Definition
on a rRNA molecule, a specific sequence of three nucleotides that is complementary to a codon triplet on mRNA.
Term
ribosomal RNA (rRNA)
Definition
the type of ribonucleic acid that, together with proteins, makes up ribosomes, the most abundant type of RNA
Term
start codon
Definition
on mRNA, specific three nucleotide the sequence to which an initiator tRNA molecule binds, starting translation of genetic information
Term
stop codon
Definition
in mRNA, one of the three triplets (UAG, UAA, UGA) that signal gene translation to stop
Term
mutation
Definition
a change in the nucleotide sequence of DNAl the ultimate source of genetic diversity
Term
reading frame
Definition
the way in which a cel's mRNA translating machinery groups the mRNA nucleotides into codons
Term
mutagenisis
Definition
the creation of a mutation
Term
mutagen
Definition
a chemical or physical agent that interacts with DNA and causes a mutation
Term
lytic cycle
Definition
a type of viral replication cycle resulting n the release of new viruses by lysis (breaking open) of the host cell
Term
lysogenic cycle
Definition
a type of bacteriophage replication cycle in which the viral genome is incorporated into the bacterial host chromosome as a prophage; new phages are not produces, and the host cell is not killed or lysed unless the viral genome leaves the host chromosome
Term
prophage
Definition
phage DNA that has inserted by genetic recombination into the DNA of a prokaryotic chromosome
Term
retrovirus
Definition
an RNA virus that reproduces by means of a DNA MOLECULE IT REVERSE TRANSCRIBES ITS rna INTO dna INSERTS THE dna INTO A CELLULAR CHROMOSOME AND THEN TRANSCRIBES MORE COPIES OF THE rna FROM THE VIRAL dna. hiv and a number of cancer causing viruses are these.
Term
reverse transcriptase
Definition
an enzyme that catalyzed the synthesis of DNA on an RNA template.
Term
AIDS
Definition
acquired immune deficiency syndrome; the latest stages of HIV infection; characterized by a reduced number of T cells usualy results in death caused by other diseases
Term
HIV
Definition
human immunodeficiency virus, the retrovirus that attacks the human immune system and causes AIDS.
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