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science of the classification of organisms |
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kingdoms plantae and animalia |
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bacteria and fungi put in the kingdom plantae |
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prokaryote intruduced for cells 'without a nucleus' |
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bacteria were separated in to the kingdom prokaryote; 5 kingdom system based on structure/function relationships |
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proposed 3 kingdom system based on cell type, and rRNA sequence relatedness |
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makes grouping according to properties that implies evolutionary relatedness (genetic commonalities) |
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genetically different within a clone |
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a population of cells derived from a single cell |
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grwon in laboratory media |
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population of cells with similar characteristics |
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a group of closely related organisms that breed among themselves |
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multicellular, no cell walls, chemohetertrophs (Energy and C source from premade organic substances we consume and digest) |
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multicellular; cellulose cell walls; usually photoautotrophic (Energy from sun and get C from CO2) |
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chemoheterotrophic; uni/multicellular; cell walls of chitin; develops from spores or hyphal fragments |
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a catchall kingdom for eukaryotes, grouped into clades based on rRNA |
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population of viruses with similar characteristics that occupies a particular ecological niche |
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placing organisms in groups of related species; list of characteristics of known organisms |
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matchin characteristics of an unknown to a list of known organisms |
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Bergey's manual of determinative bacteriology |
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for laboratory identification of bacteria, based on rRNA sequences |
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Bergey's manual for systematic bacteriology |
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Definition
for bacterial classification; based on morphology, differential staining and biochemical tests |
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gram staining and acid-fast staining |
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tests for utilization of different substances and get positive or negative and ID from that |
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involve reactions of microorganisms with specific antibodies |
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enzyme-linked immunosorbent assays where they take a well that has antibodies all over and add an unknown bacteria then add antibody with an enzyme attached and add a subsrate |
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indirect= add microbe to naked well add antibody then add a secondary antibody that has specificity for other antibody and binds causing a color change |
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separate proteins based on size by electrophoresis; technique to identify proteins from a biological source |
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used to identify bacterial species and strains by determining their susceptibility to various phages |
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DNA bas compoition (G and C %), DNA fingerprinting (electrophoresis of restriction enzyme digests), rRNA sequencing, and Polymerase chain reaction (PCR)= used to identify a small amount of microbial DNA |
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nucleic acid hybridization |
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indicates relatedness between the two: complete, partial and none |
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separate DNA and mix with fluorescent probe |
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probe technology to identify bacterial cells directly |
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tool used to differentiate organisms |
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